Comparison of the sperm quality necessary for successful intrauterine insemination with World Health Organization threshold values for normal sperm

Dickey, Richard P.; Pyrzak, Roman; Lu, Peter Y.; Taylor, Steven N.; Rye, Philip H

doi:10.1016/s0015-0282(98)00519-6

Cited by 144 publications

(92 citation statements)

References 41 publications

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“…Such limitation is shown by extensive observations. For example, it is shown that no difference could be concluded among parameters of spermatozoa concentration and motility in relation to the outcome of pregnancy, if the parameters of sperm were above certain minimum levels (31,32). In the present study, we observed a better result of routine semen analyses, as well as higher rates of fertilization and embryo cleavage in the 10 cases of the normozoospermic group, but the pregnancy rate was found lower than that of OAT patients (Table III).…”

Section: Discussionmentioning

confidence: 99%

DNA Fragmentation, Mitochondrial Dysfunction and Chromosomal Aneuploidy in the Spermatozoa of Oligoasthenoteratozoospermic Males

Liu

Tsao²,

Cheng

et al. 2004

J Assist Reprod Genet

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Purpose : This study determined the incidence of sperm nuclear DNA fragmentation, mitochondrial dysfunction, and chromosomal aneuploidy. The results were correlated with the semen analysis parameters and fertilization rates. Methods : Semen samples from 10 men showing oligoasthenoteratozoospermia (OAT) and undergoing ICSI treatment were analyzed. Another semen samples from 10 men showing normozoospermia and undergoing IVF treatment were analyzed for comparison. The samples were prepared using a two-step discontinuous Percoll gradient (80%-50%) and analyzed using a Hamilton-Thorne Integrated Visual Optical System (IVOS) Sperm Analyzer. DNA fragmentation was detected with a terminal deoxynucleotidyl transferase-mediated dUTP nick end label (TUNEL) assay. Functional integrity of mitochondria was detected using an Apoalert TM Mitochondrial Membrane Sensor Kit. Chromosomal aneuploidy was assayed by fluorescence in situ hybridization. Results : Higher sperm DNA fragmentation rate (18.8% vs. 2.8%), mitochondrial dysfunction rate (24.9% vs. 5.7%), and chromosomal aneuploidy rate (0.12% vs. 0.06%) were found in the oligoasthenoteratozoospermic patients in comparison with the normozoospermic patients. Conclusions : The result indicates that spermatozoa from oligoasthenoteratozoospermic patients contain greater DNA fragmentation, mitochondrial dysfunction, and chromosomal aneuploidy. Because extremely poor semen samples are the indication for ICSI treatment, the result indicates the importance of selecting good quality sperm for oocyte injection.

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Section: Discussionmentioning

confidence: 99%

DNA Fragmentation, Mitochondrial Dysfunction and Chromosomal Aneuploidy in the Spermatozoa of Oligoasthenoteratozoospermic Males

Liu

Tsao²,

Cheng

et al. 2004

J Assist Reprod Genet

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“…It has been reported that a PTMS of 20 × 10 6 achieves a higher pregnancy rate than 10-20 × 10 6 and that in vitro fertilization (IVF) should be adopted when PTMS is less than 10 × 10 6 [Miller et al 2002;Lu et al 2006]. Others have argued that a PTMS of 10 × 10 6 can achieve a better pregnancy rate [Dickey et al 1999;Horvath et al 1989;Van Voorhis et al 2001]. However, Huang and Lai [1996] have indicated that approximately 5 × 10 6 PTMS is even better.…”

Section: Introductionmentioning

confidence: 99%

Relationship between processed total motile sperm count of husband or donor semen and pregnancy outcome following intrauterine insemination

Dong

Sun

et al. 2011

Systems Biology in Reproductive Medicine

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“…It can be performed efficiently in the office and does not require sophisticated equipment. Studies have confirmed its efficacy for specific indications including unexplained infertility, endometriosis, male infertility, and anovulation (1)(2)(3)(4)(5). Predictive sperm parameters and threshold values with respect to semen characteristics for successful intrauterine insemination have been controversial.…”

Section: Introductionmentioning

confidence: 99%

“…Predictive sperm parameters and threshold values with respect to semen characteristics for successful intrauterine insemination have been controversial. Although the World Health Organization's (WHO) reference values for semen analysis are often used to assess sperm quality, more often than not successful pregnancies with IUI have been achieved in subfertile couples with sperm parameters below reference values listed in the WHO manual (3,4).…”

Section: Introductionmentioning

confidence: 99%

Impact of Semen Characteristics on the Success of Intrauterine Insemination

Zhao

Vlahos

Wyncott

et al. 2004

J Assist Reprod Genet

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Purpose : To evaluate the influence of sperm characteristics on the outcome of infertility treatment using intrauterine insemination (IUI). Methods : Retrospective study of 431 infertility couples who underwent 1007 IUI treatment cycles from June 1999 to October 2002. Sperm parameters before and after preparation for IUI were evaluated and correlated with pregnancy outcome. Results : Clinical pregnancy occurred in 12% of cycles and 28% of patients. Initial sperm motility and processed forward progression were independently associated with pregnancy after IUI. The mean number of cycles per patient was 4.3. Although pregnancy rate per cycle did not differ from cycle to cycle, the cumulative pregnancy rate approached plateau after five cycles. Conclusions : Sperm motility is an independent factor influencing IUI-related pregnancy. A forward progression score of 3 to 4 in a processed specimen is necessary for IUI success. The number of IUI attempts per patient should be individualized depending upon the needs of patients.

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Comparison of the sperm quality necessary for successful intrauterine insemination with World Health Organization threshold values for normal sperm

Cited by 144 publications

References 41 publications

DNA Fragmentation, Mitochondrial Dysfunction and Chromosomal Aneuploidy in the Spermatozoa of Oligoasthenoteratozoospermic Males

DNA Fragmentation, Mitochondrial Dysfunction and Chromosomal Aneuploidy in the Spermatozoa of Oligoasthenoteratozoospermic Males

Relationship between processed total motile sperm count of husband or donor semen and pregnancy outcome following intrauterine insemination

Impact of Semen Characteristics on the Success of Intrauterine Insemination

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