Comparison of the stable carbon and nitrogen isotopic values of gill and white muscle tissue of fish

Svensson, Elisabeth; Freitas, Vânia; Schouten, Stefan; Middelburg, Jack J.; Veer, Henk W. van der; Damsté, Jaap S. Sinninghe

doi:10.1016/j.jembe.2014.04.014

Cited by 23 publications

(15 citation statements)

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“…Enrichment of δ 15 N in muscle tissue has been reported by others and is often attributed to recent dietary switches or differences in turnover, assimilation rates, or amino acids incorporated (Sholto-Douglas et al, 1991, Bearhop et al, 2002, Stenroth et al, 2005, Mateo et al, 2008, Marques et al, 2011, Malpica-Cruz et al, 2013, Perkins et al 2013, and Svensson et al, 2014). Additionally, the enrichment of δ 13 C in muscle tissue observed in this study can be attributed to differences in tissue lipid content, since lipids are depleted in δ 13 C compared to carbohydrates and protein (DeNiro and Epstein, 1978, Stenroth et al, 2006 and Mateo et al, 2008).…”

Section: Discussionmentioning

confidence: 75%

Influence of sample preparation on estuarine macrofauna stable isotope signatures in the context of contaminant bioaccumulation studies

Curtis

Buggé

Buckman

et al. 2017

Journal of Experimental Marine Biology and Ecology

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The ratios of stable isotopes of carbon and nitrogen provide important information on food sources of aquatic organisms and trophic structure of aquatic food webs. For many studies, trophic position and food source are linked to bioaccumulation and trophic transfer of contaminants from prey to predators. In these cases, it is useful to use measurements on whole organisms to make direct comparisons of contaminant bioaccumulation and food web attributes. There is a great deal of variation in methods used for stable isotope analysis, particularly in the selection of tissue type and sample preparation prior to stable isotope analysis. While there have been aquatic studies that examined methodological differences, few have focused on estuarine organisms. In this study, the effects of depuration and tissue dissection on the stable isotope enrichment of common estuarine invertebrates and fish were examined. Homogenized tissues of non-depurated whole organisms were compared to dissected muscle tissue or depurated whole organisms. A 24 h depuration did not change the mean δ15N and δ13C values for most species examined. Additionally, as expected, significant differences in carbon and nitrogen signatures were found when muscle tissues were compared to whole organisms. However, differences were small enough that food source as inferred by δ13C or trophic level as inferred from δ15N would not be inaccurately represented (differences of <1.9‰ for δ13C and <1.2‰ for δ15N). The results of this study suggest that for these common estuarine fish and macroinvertebrates, stable isotopes ratios of samples can be analyzed without depuration in the same way as samples for contaminant analysis, but differences in tissue types must be taken into account when combining data from different sources.

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Section: Discussionmentioning

confidence: 75%

Influence of sample preparation on estuarine macrofauna stable isotope signatures in the context of contaminant bioaccumulation studies

Curtis

Buggé

Buckman

et al. 2017

Journal of Experimental Marine Biology and Ecology

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“…The mixture was extracted and centrifuged (3000 rpm, 10 min), and the upper organic solvent layer was transferred to a flask using a pipette. The solvent was evaporated to dryness under a stream of N 2 at room temperature and weighed, and the lipid content was calculated as percentage of the mass of unextracted freeze-dried tissue (Svensson et al, 2014). The extracted tissue has been dried under a stream of N 2 at room temperature and was used to measure 13 C. The FAs were transesterified to FA methyl esters (FAMEs) in a mixture of methanol (containing 5% HCl) and n-hexane at 50 • C for approximately 12 h (Eder, 1995).…”

Section: Fatty Acid Analysismentioning

confidence: 99%

“…The results were normalized to the Vienna Pee Dee Belemnite standard (PDB) for δ 13 C and to atmospheric N (N 2 ) for δ 15 N (Cherel et al, 2010). The stable isotope ratios are expressed in δ notation of units per mill as follows (Svensson et al, 2014):…”

Section: Stable Isotope Analysismentioning

confidence: 99%

“…Thus, they can become essential dietary components for higher trophic level species and are used as dietary tracers in food web studies (Connan et al, 2010). Consumers preferentially retain varying levels of 13 C and 15 N in their tissues during metabolic processing, and therefore δ 13 C bulk and δ 15 N values can reveal quantitative estimates of the trophic positions of food web components (DeNiro and Epstein, 1977;Petursdottir et al, 2008;Svensson et al, 2014;Choy et al, 2015;Gloeckler, 2016). δ 13 C bulk is mainly used to indicate the foraging habitats of predators, while δ 15 N indicates the trophic position of a consumer (Fry, 1988;Cherel et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Trophic Interactions of Mesopelagic Fishes in the South China Sea Illustrated by Stable Isotopes and Fatty Acids

Wang

Chen

et al. 2019

Front. Mar. Sci.

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As the most abundant fishes and the least investigated components of the open ocean ecosystem, mesopelagic fishes play an important role in biogeochemical cycles and hold potentially huge fish resources. There are major gaps in our knowledge of their biology, adaptations and trophic dynamics and even diel vertical migration (DVM). Here we present evidence of the variability of ecological behaviors (migration and predation) and trophic interactions among various species of mesopelagic fishes collected from the South China Sea indicated by isotopes (δ 13 C, δ 15 N), biomarker tools [fatty acids (FAs), and compound-specific stable isotope analysis of FAs (CSIA)]. Higher lipid contents of migrant planktivorous fishes were observed with average values of 35%, while others ranged from 22 to 29.5%. These high lipids contents limit the application of δ 13 C bulk (bulk-tissue δ 13 C) as diet indicator; instead δ 13 C extraction (the lipid extracted δ 13 C) values were applied successfully to reflect dietary sources. The δ 15 N values of non-migrant planktivorous and piscivorous fishes varied in a narrow range (10.0-10.8). This small difference may be caused by the low temperature in deep ocean, which results in low metabolic rates of nitrogen. The piscivorous fishes had higher FA ratios of DHA (22:6n−3)/EPA (20:5n−3) than planktivorous fishes, which mirrored their potential carnivory. CSIA can reveal small changes in biochemical composition and distinguishes between possible dietary sources such as between the non-migrant piscivorous and the semi-migrant piscivorous fishes with significantly different δ 13 C values of the 20:4n−6 and 20:5n−3 FAs (p < 0.01). Mesopelagic fishes exhibit complex trophic interactions revealed by molecular and CSIA tools and play a vital role in the marine "biological pump." This knowledge is important to comprehensively evaluate the role of mesopelagic fishes in global carbon budgets. Keywords: mesopelagic fishes, δ 13 C extraction , δ 15 N, fatty acids, δ 13 C of individual fatty acids, South China Sea Abbreviations: δ 13 C bulk, bulk-tissue δ 13 C; δ 13 C extraction, lipid extracted δ 13 C; CSIA, compound-specific stable isotope analysis; DHA (22:6n−3), docosahexaenoic acid; DVM, diel vertical migration; EPA (20:5n−3), eicosapentaenoic acid; FA, fatty acids.

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“…A alta biodiversidade encontrada em sistemas estuarinos, provavelmente promove grande variedade de presas para cada predador Kehrig et al, 2013). Além disso, os isótopos foram quantificados no tecido muscular dorsal dos peixes e dos predadores de topo, portanto, os resultados integram a dieta de longo prazo (meses a um ano), e refletem seus hábitos alimentares (Das et al, 2003;Kehrig et al, 2013;Svensson et al, 2014), sugerindo que as espécies estão assimilando diversos itens alimentares de táxons diferentes. A amplitude dos valores detectados de carbono orgânico total (COT) foi de 0,20 a 1,13 para sedimentos do CEP, 0,22 a 2,57 do SELCI e de 0,34 a 3,02 do CESSV.…”

Section: Resultados Semelhantes Aos Do Presente Estudo Foram Verificaunclassified

Bioacumulação e biomagnificação de metais pesados em teias tróficas de estuários do sul-sudeste do Brasil

Trevizani¹

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Comparison of the stable carbon and nitrogen isotopic values of gill and white muscle tissue of fish

Cited by 23 publications

References 48 publications

Influence of sample preparation on estuarine macrofauna stable isotope signatures in the context of contaminant bioaccumulation studies

Influence of sample preparation on estuarine macrofauna stable isotope signatures in the context of contaminant bioaccumulation studies

Trophic Interactions of Mesopelagic Fishes in the South China Sea Illustrated by Stable Isotopes and Fatty Acids

Bioacumulação e biomagnificação de metais pesados em teias tróficas de estuários do sul-sudeste do Brasil

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