Comparison of the Structures of Tooth Germs Transplanted into the Subcutaneous Tissue or the Kidney Capsule of Mice

Higuchi, Yoshinori; Yamamoto, Hitoshi; Cai, Jinglei; Suzuki, Kunihiro; Jung, Han‐Sung; Kozawa, Yukishige

doi:10.5466/ijoms.7.27

Cited by 2 publications

(1 citation statement)

References 20 publications

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“…According to these reports, a new epithelial-mesenchymal interaction may be acquired by the reaggregation system. Moreover, recently it is reported that transplanted tooth using normal tooth germ of mandibular first molar in mice has only one tooth root, although cementum distribution pattern is similar to normal molar in vivo 13) These results suggest that the microenvironment surrounding tooth germ may be an important factor for multi-root formation.…”

Section: Discussionmentioning

confidence: 65%

Periodontal Tissue Formation by Reaggregation System in Mice

Yamamoto

Cai

Cho

et al. 2009

J. Hard Tissue Biology.

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Abstract:The reaggregation system is a special technique developed to clarify the essential characteristics of the mesenchyme. In this study, the reaggregation system was performed using mouse lower first molar at E13.5 and the reaggregated tooth germs were transplanted into the kidney capsule for 6 weeks to investigate tooth root formation. The reaggregated tooth was surrounded by bone. The reaggregated tooth germ produced a single thick root like a taurodontium (cuneiform tooth), although mouse lower first molar has two roots in vivo. Histologically, the reaggregated mesenchyme could form not only tooth elements including enamel, dentin, dental pulp and cementum, but also periodontal tissue including periodontal ligament-like structure and alveolar bone-like structure. The periodontal ligament-like structure has a regular width between the cementum and alveolar bone-like structure. Moreover, Sharpy's fiber-like structure was inserted into the cementum and alveolar bone-like structure. In conclusion, the reaggregated mesenchyme has the ability to form both tooth and periodontal tissue without information from the epithelium. In addition, this reaggregation system may be a useful method for regenerative dentistry, since tooth and periodontal tissue can be produced en bloc.

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Section: Discussionmentioning

confidence: 65%

Periodontal Tissue Formation by Reaggregation System in Mice

Yamamoto

Cai

Cho

et al. 2009

J. Hard Tissue Biology.

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Wnt/β-catenin Promotes Cementum Apposition in Periodontal Regeneration

Ono,

Kaku,

Thant

et al. 2024

J Dent Res

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Regeneration of periodontal tissue, particularly the cementum–periodontal ligament (PDL)–bone complex, has long been challenging because the differentiation kinetics of cells and the molecular pathways contributing to the regeneration process are largely unknown. We aimed to evaluate the cell behavior and molecular pathways that contribute to periodontal tissue regeneration in vivo. We analyzed the process of periodontal tissue regeneration through subrenal capsule transplantation of immediately extracted molars in mice. We showed that the regenerated periodontal tissue in the subrenal capsule was morphologically comparable to the intact periodontal tissue, with increased cellular cementum thickness in the apical region. Cell tracing analysis revealed that the cells comprising the regenerated periodontal tissue were derived from transplanted teeth and were indispensable for periodontal tissue regeneration, whereas recipient mouse-derived cells partly contributed to angiogenesis. Bioinformatics analysis based on the gene expression profile in the transplanted teeth indicated that Wnt/β-catenin signaling is involved in periodontal tissue regeneration, which was further confirmed through β-catenin immunohistochemistry. Moreover, the constitutive activation of β-catenin in the cells of transplanted teeth was found to promote accelerated cellular cementum apposition, while the conditional knockout of β-catenin in the cells of transplanted teeth suppressed cellular cementum apposition. Notably, the manipulation of Wnt/β-catenin signaling did not interfere with the bone–PDL–cementum complex, while endogenous osteoclast activity was affected in bone. Our results demonstrated the essential roles of endogenous PDL cells in periodontal tissue regeneration and that Wnt/β-catenin signaling is involved in this process, particularly cellular cementum apposition. Hence, controlling this pathway could promote cementum regeneration, which is a critical process for the regeneration of the cementum–PDL–bone complex. This study provides novel insights into cell behavior and signaling pathways that will advance practical periodontal tissue regeneration.

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Comparison of the Structures of Tooth Germs Transplanted into the Subcutaneous Tissue or the Kidney Capsule of Mice

Cited by 2 publications

References 20 publications

Periodontal Tissue Formation by Reaggregation System in Mice

Periodontal Tissue Formation by Reaggregation System in Mice

Wnt/β-catenin Promotes Cementum Apposition in Periodontal Regeneration

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