Comparison of the toxic effects of different mycotoxins on porcine and mouse oocyte meiosis

Lu, Yujie; Zhang, Yue; Liu, Jia‐Qian; Zou, Peng; Jia, Lianshun; Su, Yongteng; Sun, Yurong; Sun, Shao‐Chen

doi:10.7717/peerj.5111

Cited by 26 publications

(16 citation statements)

References 26 publications

(32 reference statements)

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“…OTA affected oocyte maturation only at micromolar concentrations. These findings are in agreement with previously reported studies in mice (Huang and Chan 2014 ; Jia et al 2019 ) and pigs (Lu et al 2018 ). However, data on meiosis progression with effects on earlier meiotic stages were not reported in those studies.…”

Section: Discussionsupporting

confidence: 94%

“…This result was unexpected since mouse oocytes exposed to OTA during IVM exhibited impaired embryonic development (Huang and Chan 2014 ; Jia et al 2019 ). A possible explanation for these discrepancies may lie in species-specific effects (Lu et al 2018 ). At any tested concentration, OTA did not affect blastocyst morphology and growth and, increased the apoptotic index only at nanomolar concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…OTA significantly impaired oocyte maturation, and decreased IVF rates and embryonic development in vitro. A subsequent comparative study between mouse and porcine oocytes confirmed a significant reduction of the maturation rate in vitro in both species, but porcine oocytes were more sensitive to OTA than mouse oocytes (Lu et al 2018 ). A recent study (Lan et al 2019 ), using high-throughput technologies, showed that OTA exposure altered the expression of multiple genes in oocytes, indicating its molecular effects on oocyte maturation.…”

Section: Introductionmentioning

confidence: 97%

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Ochratoxin A affects oocyte maturation and subsequent embryo developmental dynamics in the juvenile sheep model

et al. 2020

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The genotoxic and nephrotoxic mycotoxin Ochratoxin A (OTA) has also been reported to have adverse effects on oocyte maturation and embryo development. Previous studies on the effects of OTA on female fertility have used micromolar concentrations, but no information is available to date on effects in a more relevant nanomolar range. This study used a juvenile sheep model to evaluate the effects of oocyte exposure to low levels of OTA on maturation, fertilization, and embryo development. Further, it was investigated whether different mechanisms of action of OTA could be responsible for varying toxic effects at different levels of exposure. Cumulus-oocyte-complexes (COCs) were exposed to 10 μmol/L–0.1 nmol/L OTA during in vitro maturation and evaluated for cumulus viability, oocyte maturation, and bioenergetic/oxidative status. COCs were subjected to in vitro fertilization, embryo culture, and embryo quality assessment via morphology, viability, bioenergetic/oxidative status, and time-lapse monitoring. At micromolar concentrations, OTA induced cytotoxic effects, by reducing cumulus expansion and oocyte maturation. OTA altered temporospatial dynamics of zygote pronuclear formation and embryo morphokinetics. Blastocysts, even morphologically normal, were found to undergo collapse events, which were probably related to boosted blastocyst mitochondrial activity. At nanomolar concentrations, OTA did not affect COC morpho-functional parameters, but impaired oocyte ability to prevent polyspermy and increased blastocyst apoptosis. In conclusion, in the female germ cell, cytotoxic nonspecific effects characterize OTA-induced toxicity at high exposure levels, whereas fine tuning-mode effects, not associated with altered cell viability and integrity, characterize OTA toxic action at low levels.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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Ochratoxin A affects oocyte maturation and subsequent embryo developmental dynamics in the juvenile sheep model

et al. 2020

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“…Previous studies on porcine oocytes have shown that 10 μ M ZEA significantly affects oocyte maturation (Lu et al, 2018). Therefore after parthenogenetic activation, we first examined the effects of 5 or 10 μM ZEA treatment on embryonic development.…”

Section: Resultsmentioning

confidence: 99%

Protective Effects of Melatonin Against Zearalenone Toxicity on Porcine Embryos in vitro

Zhang

Sun

et al. 2019

Front. Pharmacol.

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Zearalenone (ZEA) is an estrogenic mycotoxin produced by Fusarium fungi commonly found in corn, wheat, and other cereals which can infect food and feed commodities, and ZEA mainly has reproductive toxicity which causes widely reproductive disorders in pigs and other animals. However, the toxicity and the functional ways of ZEA on early embryo development is still unclear. In present study we showed that exposure to ZEA (10 μM) significantly decreased the 2-cell and blastocyst developmental rate in porcine early embryos in vitro . ZEA treatment resulted in the occurrence of oxidative stress, showing with increased reactive oxygen species (ROS) level, following with aberrant mitochondrial distribution. Moreover, we found positive signals of γH2A.X in the ZEA-treated embryos, indicating that ZEA induced DNA damage, and the increased autophagy confirmed this. These results suggested that ZEA induced oxidative stress, which further caused mitochondria dysfunction and DNA damage on early embryonic development. We next investigated the effects of melatonin on the ZEA-treated embryo development, and we found that melatonin supplementation could significantly ameliorate ZEA-induced oxidative stress, aberrant mitochondria distribution and DNA damage. In all, our results showed that ZEA was toxic for porcine embryos cultured in vitro and melatonin supplementation could protect their development from the effects of ZEA.

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“…PPT has been shown to cause oxidative stress and apoptosis in porcine oocytes (Jiang et al, 2020). Mycotoxins, such as AFB1, deoxynivalenol, and HT-2 toxins, could also cause oxidative stress in oocytes and other cell types (Yang et al, 2014;Zhang et al, 2016;Lu et al, 2018). In addition, the excessive accumulation of histone H2A variant H2AX in cells undergoing DNA damage can increase the activity of NADP (H) oxidase (NOx), thereby increasing the level of ROS.…”

Section: Discussionmentioning

confidence: 99%

Podophyllotoxin Exposure Causes Spindle Defects and DNA Damage-Induced Apoptosis in Mouse Fertilized Oocytes and Early Embryos

Liao

Wei

et al. 2020

Front. Cell Dev. Biol.

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Podophyllotoxin (PPT) is a kind of lignans extracted from the roots and stems of the genus Podophyllum from the tiller family, and it has been widely used in the treatment of condyloma acuminatum, multiple superficial epithelioma in the clinics. However, PPT has been reported to be toxic and can cause liver defects and other organ poisoning. In addition, emerging evidences also indicate that PPT has reproductive toxicity and causes female reproduction disorders. In this study, we used fertilized oocytes and tried to explore the effects of PPT on the early embryonic development with the mouse model. The results showed that exposure to PPT had negative effects on the cleavage of zygotes. Further analysis indicated that PPT could disrupt the organization of spindle and chromosome arrangement at the metaphase of first cleavage. We also found that PPT exposure to the zygotes induced excessive reactive oxygen species (ROS), suggesting the occurrence of oxidative stress. Moreover, in the PPT-exposed embryos, there was positive γH2A.X and Annexin-V signals, indicating that PPT induced embryonic DNA damage and early apoptosis. In conclusion, our results suggested that PPT could affect spindle formation and chromosome alignment during the first cleavage of mouse embryos, and its exposure induced DNA damage-mediated oxidative stress which eventually led to embryonic apoptosis, indicating the toxic effects of PPT on the early embryo development.

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Comparison of the toxic effects of different mycotoxins on porcine and mouse oocyte meiosis

Cited by 26 publications

References 26 publications

Ochratoxin A affects oocyte maturation and subsequent embryo developmental dynamics in the juvenile sheep model

Ochratoxin A affects oocyte maturation and subsequent embryo developmental dynamics in the juvenile sheep model

Protective Effects of Melatonin Against Zearalenone Toxicity on Porcine Embryos in vitro

Podophyllotoxin Exposure Causes Spindle Defects and DNA Damage-Induced Apoptosis in Mouse Fertilized Oocytes and Early Embryos

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