Comparison of the two blood culture systems, Bactec 9240 and BacT/Alert 3D, in the detection of Candida spp. and bacteria with polymicrobial sepsis

Klingspor, Lena; Muhammed, S. A.; Özenci, Volkan

doi:10.1007/s10096-012-1650-6

Cited by 15 publications

(14 citation statements)

References 14 publications

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“…The rationale for choosing Bactec MICF bottles for these experiments was that in experiments evaluating the general applicability of different commercial blood culture systems for detecting Candida spp., Bactec MICF bottles, which include supplements specifically adapted for the growth of fungi, were shown to be more sensitive and to detect Candida spp. faster than most other conventional blood culture media (4)(5)(6)(7)(8). This finding was confirmed by the results of our study, since for controls inoculated without antifungal agents, we found reduced median TTPs in MICF bottles compared to those in PAF bottles (21.1 h versus 24.4 h for C. albicans and 22.0 h versus 43.2 h for C. glabrata, respectively).…”

Section: Discussionsupporting

confidence: 90%

“…Therefore, some studies have evaluated the commercial Bactec system for its ability to detect Candida bloodstream infections. With this system, Bactec Mycosis IC/F (MICF) blood culture bottles, which are specifically adapted to the growth of Candida spp., were demonstrated to have a significantly shorter time to positivity (TTP) and a higher Candida detection rate than conventional (an)aerobic media (such as the Bactec Plus Aerobic F [PAF] and Bactec anaerobic bottles) (4)(5)(6)(7)(8).…”

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confidence: 99%

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Evaluation of Bactec Mycosis IC/F and Plus Aerobic/F Blood Culture Bottles for Detection of Candida in the Presence of Antifungal Agents

et al. 2013

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Clinical practice guidelines recommend performing follow-up cultures for patients with candidemia in order to determine the time when Candida is cleared from the bloodstream. Since this requires culturing blood samples from patients undergoing antifungal treatment, we evaluated two blood culture bottles (the Bactec Mycosis IC/F [MICF], specifically adapted to the growth of fungi, and the Bactec Plus Aerobic/F [PAF], containing resins to inactivate anti-infective agents) for their effectiveness in detecting Candida albicans and Candida glabrata when seeded in concentrations of 1 CFU/ml and 10 CFU/ml, respectively, together with human whole blood and various antifungal agents in therapeutic peak serum concentrations (C max ). Significant differences between the MICF and PAF vials for the detection of Candida spp. were found when inoculated with caspofungin (0/12 versus 8/12) (P < 0.001) or amphotericin B (3/12 versus 12/12) (P < 0.001). Inoculation of fluconazole or voriconazole did not influence the effectiveness of detection in the MICF and PAF bottles (P ‫؍‬ 1.0). Neither the MICF nor the PAF bottles detected Candida spp. reliably when seeded together with anidulafungin (1/12 versus 1/12) (P ‫؍‬ 1.0) or micafungin (0/12 versus 1/12) (P ‫؍‬ 1.0). The times to positivity of both bottles were significantly prolonged when antifungal agents were added compared to those of controls without antimycotic drugs (P < 0.001). Overall, the results of this in vitro study indicate that the PAF bottles detected Candida spp. more reliably than the MICF bottles when supplemented with certain antifungal agents. Consequently, clinical studies should evaluate whether this holds true when blood cultures from patients undergoing antifungal treatment are performed.

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Section: Discussionsupporting

confidence: 90%

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confidence: 99%

Evaluation of Bactec Mycosis IC/F and Plus Aerobic/F Blood Culture Bottles for Detection of Candida in the Presence of Antifungal Agents

et al. 2013

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“…Some authors [ 8 , 9 ], who reported a positivity of Aerobic as high as 90–98 %, and Anaerobic ranging between 10 and 15 %, found that almost all vials which were signaled by the BACTEC system as negative proved to be positive after terminal subculture. Conversely, according to the data collected by Klingspor et al [ 6 ], there was no growth observed after the subculture of negative vials; thus, there was no false negativity. In the current study, all isolates used to prepare simulated cultures were grown on Aerobic and Mycosis, but the positivity ratio of Anaerobic media was low (9 %).…”

Section: Discussionmentioning

confidence: 92%

“…In the case of clinical blood samples, the false-negative results are regarded rather seldom, but were reported in some media of the BACTEC as well as BacT/ALERT systems [ 13 , 14 ]. Interestingly, in the polymicrobial model of infection, detection of yeasts by Anaerobic media was enhanced up to 79 % [ 6 ]. This rule does not apply to Aerobic media.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of blood culture media for the detection of fungi

Nawrot

Kowalska−Krochmal

Sulik-Tyszka

et al. 2014

Eur J Clin Microbiol Infect Dis

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The aim of this study was to compare the utility of BACTEC™ Mycosis-IC/F (Mycosis), BACTEC™ Plus Aerobic/F (Aerobic), and BACTEC™ Plus Anaerobic/F (Anaerobic) media in the detection of fungi from simulated (obtained by the inoculation of tested media first with sterile sheep’s blood and subsequently with one of 60 clinical yeast isolates) and clinical blood samples, taken during routine diagnostic examination in two hospitals. All tested strains grew on Mycosis as well as Aerobic bottles, and the time to detection obtained for Mycosis was significantly shorter (p < 0.05). The largest differences in the time to positivity was found for Candida glabrata and Cryptococcus neoformans, when Mycosis preceded Aerobic in 20–48 h (mean 35.5 h) and 0.7–64 h (mean 24 h), respectively. On the contrary, C. krusei were detected earlier in Aerobic media. In clinical samples, the detection of C. glabrata was also significantly faster in Mycosis than in Aerobic (29.22 ± 11.48 h compared to 86 ± 40 h). The media complement each other and, in 45 % of clinical examination sets, a single positive medium was noted (25 % in Mycosis and 19 % in Aerobic). The study proved that both Aerobic and Mycosis media serve as the correct condition for the culture of fungi and that they varied significantly in the detection time of clinically important species. This result could suggest that the simultaneous use of Aerobic as well as Mycosis media may improve the time of diagnosis in many patients, especially those infected with C. glabrata or C. neoformans.

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“…Previously, the effects of the simultaneous presence of bacteria ( Staphylococcus aureus and Escherichia coli ) and Candida spp. ( C. albicans and C. glabrata ) on blood culture results were evaluated [6, 7]. They showed that bacterial growth could hide fungal growth in non-selective medium.…”

Section: Introductionmentioning

confidence: 99%

Does concomitant bacteraemia hide the fungi in blood cultures? An in vitro study

Önder

Alpaslan

et al. 2020

Journal of Medical Microbiology

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Introduction. Polymicrobial infections including yeasts and bacteria are not rare and patients with polymicrobial bloodstream infection have higher early and overall case fatality rates. The diagnosis of invasive fungal and bacterial infections is mainly based on blood culture. Aim. The aim was to reveal the effect of concomitant bacteraemia on the detection of fungi from blood cultures in the presence of polymicrobial bloodstream infections involving Candida and non-Candida fungi and to show the superiority of blood culture bottles including selective fungal media in such situations. Methodology. Twenty-four polymicrobial bloodstream infection models – involving one fungus and one bacterium – were constituted by using clinical blood culture isolates ( Escherichia coli , Staphylococcus aureus , Pseudomonas aeruginosa , Candida albicans, Candida glabrata, Fusarium solani and Trichosporon asahii). The Plus Aerobic/F (PAF) and Mycosis IC/F (MICF) culture bottles were used with the BACTEC 9240 device. After a bottle signalled positive, direct microscopic examination and subcultures on agar plates were performed. Results. All of fungi that were inoculated alone and in combination were detected by both direct microscopic examination and subcultures on agar plates from MICF bottles, whereas direct microscopic examination only revealed the bacterial agents from PAF bottles including combinations. Furthermore, fungal growth was hidden by bacterial growth on blood agar subcultures from PAF bottles including combinations of F. solani, C. glabrata or T. asahii with bacteria. Conclusion. Blood culture bottles including selective fungal media that can allow selective growth of fungi and earlier detection of some species should be preferred in addition to non-selective blood culture bottles, especially in specific patient populations. Further, the use of selective agar plates such as inhibitory mould agar may contribute to the solution of this problem in clinical laboratories.

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Comparison of the two blood culture systems, Bactec 9240 and BacT/Alert 3D, in the detection of Candida spp. and bacteria with polymicrobial sepsis

Cited by 15 publications

References 14 publications

Evaluation of Bactec Mycosis IC/F and Plus Aerobic/F Blood Culture Bottles for Detection of Candida in the Presence of Antifungal Agents

Evaluation of Bactec Mycosis IC/F and Plus Aerobic/F Blood Culture Bottles for Detection of Candida in the Presence of Antifungal Agents

Evaluation of blood culture media for the detection of fungi

Does concomitant bacteraemia hide the fungi in blood cultures? An in vitro study

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