Comparison of the Urinary Bladder Base and Detrusor to Cholinergic and Histaminergic Receptor Activation in the Rabbit

Buren, George A. Van; Anderson, Gail S.

doi:10.1159/000137242

Cited by 10 publications

(2 citation statements)

References 7 publications

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“…It was determined that in the presence of the H1 antagonist pyrilamine, increases in baseline tension in response to histamine were significantly inhibited in both U&LP and detrusor preparations. This is consistent with past studies showing pyrilamine’s effectiveness in inhibiting contractions to histamine in rabbit detrusor tissue 30 and cultured human detrusor cells 20 . It is known that U&LP is capable of developing spontaneous phasic contractions in absence of any stimulation 1 .…”

Section: Discussionsupporting

confidence: 93%

Histamine modulation of urinary bladder urothelium, lamina propria and detrusor contractile activity via H1 and H2 receptors

Štromberga

Chess-Williams

Moro

2019

Sci Rep

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The mechanisms underlying bladder contractile disorders such as overactive bladder are not fully understood, and there is limited understanding of the receptor systems modulating spontaneous bladder contractions. We investigated the potential for histamine to have a role in mediating contractility of the urothelium with lamina propria (U&LP) or detrusor via the H1-H4 histamine receptor subtypes. Isolated strips of porcine U&LP or detrusor smooth muscle were mounted in gassed Krebs-bicarbonate solution and responses to histamine obtained in the absence and presence of selective receptor antagonists. The presence of histamine increases the frequency of U&LP spontaneous phasic contractions and baseline tensions. In response to histamine, H1-antagonists pyrilamine, fexofenadine and cyproheptadine were effective at inhibiting contractile responses. Cimetidine (H2-antagonist) enhanced increases in baseline tension in response histamine, whereas amthamine (H2-agonist) induced relaxation. Although thioperamide (H3/H4-antagonist) increased baseline tension responses to histamine, selective H1/H2-receptor antagonism revealed no influence of these receptors. In detrusor preparations, pyrilamine, fexofenadine and cyproheptadine were effective at inhibiting baseline tension increases in response to histamine. Our findings provide evidence that histamine produces contractile responses both in the U&LP and detrusor via the H1-receptor, and this response is significantly inhibited by activation of the H2-receptor in the U&LP but not the detrusor.

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Section: Discussionsupporting

confidence: 93%

Histamine modulation of urinary bladder urothelium, lamina propria and detrusor contractile activity via H1 and H2 receptors

Štromberga

Chess-Williams

Moro

2019

Sci Rep

View full text Add to dashboard Cite

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“…Elm6r, 1978). The nature of this non-cholinergic part of the contraction has not been established, but several possible transmitter substances have been proposed, e.g., ATP, prostaglandins, and histamine (Burnstock et al, 1978;Andersson and Forman, 1978;van Buren and Andersson, 1979). The present results suggest contractile SP actions on the urinary bladder involving cholinergic as well as non-cholinergic transmission.…”

Section: Discussionmentioning

confidence: 57%

In vivo motor effects of substance P on the rat urinary bladder

Berggren

Ahlman

Dahlström³

et al. 1988

J. Neural Transmission

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Intravesical pressure recordings of the urinary bladder in anesthetized rats were performed and the role of substance P (SP) in the motor control of this organ was evaluated. Regional injection of SP (0.4 nmoles i.a.) into the superior vesical artery elicited a prompt bladder contraction; this motor response was dosedependent. The detrusor contraction could be completely inhibited by a SP-analogue, (D-Pro2, D-Trp7,9)-SP (45-90 nmoles i.a.). Furthermore, the detrusor contraction evoked by preganglionic stimulation of the pelvic nerves was partially inhibited by the same antagonist in a higher dose (65% reduction at a total dose of 150-300 nmoles). The contractile response to SP (0.5 nmoles i.a.) was also significantly reduced after blockade of muscarinic receptors with atropine (50% reduction at 1 mg/kg i.a.) or after ganglionic blockade with hexamethonium (75% reduction at 25 mg/kg i.v. + 50 mg/kg hr i.a.). Immunocytochemical studies demonstrated the occurrence of SP-immunopositive nerve terminals in the detrusor part of the rat urinary bladder. Based on these findings it is suggested that SP may act as a neurotransmitter/modulator in this organ. The mechanism of action for SP on the detrusor seems to be complex and may involve ganglionic transmission via both types of cholinoceptors as well as direct activation of smooth muscle.

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Central cholinergic mechanisms in L-DOPA induced hyperactive urinary bladder of the rat

1982

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The involvement of central and peripheral cholinergic structures in the mediation of a centrally induced hyperactive urinary bladder response to L-2,3-dihydroxyphenylalanine (L-DOPA), after peripheral decarboxylase inhibition, registered by a cystometric procedure, has been analysed pharmacologically in anaesthetised rats. The urinary bladder response to L-DOPA was unchanged after blockade of cholinergic receptors with methylscopolamine, diminished after atropine and totally inhibited after hexamethonium. In addition, activation of muscarinic receptors in the pontine-mesencephalic brain region with oxotremorine after methylscopolamine pretreatment generates a hyperactive urinary bladder response, mediation of which seems to be independent of endogenous catecholamine stores. It is suggested that cholinergic receptors in the pontine-mesencephalic brain region are of importance for regulation of urinary bladder function in the rat. Furthermore, the bladder hyperactivity induced by L-DOPA might be propagated via muscarinic receptors in this brain area, and mediated peripherally via cholinergic receptors in the autonomic ganglia, but in the bladder detrusor via non-cholinergic receptors.

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Comparison of the Urinary Bladder Base and Detrusor to Cholinergic and Histaminergic Receptor Activation in the Rabbit

Cited by 10 publications

References 7 publications

Histamine modulation of urinary bladder urothelium, lamina propria and detrusor contractile activity via H1 and H2 receptors

Histamine modulation of urinary bladder urothelium, lamina propria and detrusor contractile activity via H1 and H2 receptors

In vivo motor effects of substance P on the rat urinary bladder

Central cholinergic mechanisms in L-DOPA induced hyperactive urinary bladder of the rat

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