Comparison of the use of mussels and semipermeable membrane devices for monitoring and assessment of accumulation of mutagenic pollutants in marine environment in combination with a novel microbiological mutagenicity assay

Abstract

A novel microbiological mutagenicity assay, based on bioluminescence of a marine bacterium Vibrio harveyi mutant strain, potentially suitable for monitoring and assessment of mutagenic pollution of marine environment, has been described recently. Here, we tested the use of this assay, in combination with either mussels (Mytilus sp.) or semipermeable membrane devices (SPMDs), in assessment of accumulation of mutagens in marine water (samples of Baltic Sea water were tested). Either similar results were obtained in both systems or higher signals in the SPMD-based system were detected, depending on the tested water samples. We conclude that the use of both mussels and SPMDs in combination with the V. harveyi bioluminescence mutagenicity assay is a method suitable for monitoring and assessment of accumulation of mutagenic pollutants in marine environment, but in some cases the SPMD-based system may provide a more sensitive test.

Applications for Passive Sampling of Hydrophobic Organic Contaminants in Water—A Review

“…[173] Coastal waters PAHs SPMDs deployed alone and alongside mussels and analyzed chemically and with several bioassays to monitor the concentrations of a range of environmental toxicants [259,260] Calibration and passive sampler performance In situ calibration of PRC spiked SPMDs in a range of waters and for various exposures to determine the uptake rates of a range of HOCs, including through deployment alongside biota (crayfish), with addition of antifouling agents, under diffing water velocities and the development of predictive models.…”

Chemicals used for maintenance of wood rafts in mussel farms: evaluation of their potential toxic risk to mussel culture

“…However, the survival of S. typhimurium is dramatically reduced in marine water (Czyz et al 2002, Ohe et al 2004, so other mutagenicity assays using marine bacteria have been developed based on detection of reversion of dark mutants of Vibrio fischeri and V. harveyi or on detection of neomycin-resistant mutants of V. harveyi strains (Kwan et al 1990, Czyz et al 2000, 2002, Podgórska & Wegrzyn 2006, Słoczynska et al 2010. In the present study, the latter assay was Tables 1 & 2 selected for mutagenicity analysis of the cleaning mixtures used in mussel farms because of its greater sensitivity, simplicity, and low cost (Johnson 1992, Podgórska & Wegrzyn 2006, Chec et al 2008, Ruiz et al 2013. Taking this into account, the mutagenic capacity of the water-accommodated fractions from the tars and the waterproof paint, before and after being metabolised by mouse liver microsomes, were tested in comparison to the mutagenic activity of BaP.…”

“…However, this bacterium has low survival in the presence of salts, so marine bacteria adapted to high osmotic pressures seem more suitable to evaluate chemical toxicity in marine environments (Czyz et al 2002, Ohe et al 2004, Podgórska & Wegrzyn 2007, Chec et al 2008. For this reason, other mutagenicity assays using marine bacteria have been developed, such as the one based upon detection of neomycin-resistant mutants of Vibrio harveyi.…”

Bioacumulación de hidrocarburos aromáticos policíclicos en Anadara tuberculosa (Sowerby, 1833) (Arcoida: Arcidae)

“…Para realizar estudios de seguimiento a este tipo de contaminantes se ha recurrido al uso de bivalvos como organismos biomonitores (Page et al 2005;Chęć et al 2008), pues a través del proceso de fi ltración se favorece la bioacumulación de los diferentes xenobióticos presentes en el ambiente, siendo importantes en evaluaciones de las condiciones del medio que habitan.…”