Comparison of the uterine inflammatory response to frozen-thawed sperm from high and low fertility bulls

Donnellan, E.M.; O’Brien, Megan B.; Meade, Kieran G.; Fair, Seán

doi:10.1016/j.theriogenology.2021.09.012

Cited by 7 publications

(4 citation statements)

References 35 publications

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“…The most likely reason for this is that the recruitment of PMNs is a general response of the uterus to any invaders including sperm. Moreover, as PMNs can bind more than one sperm, we postulate that PMNs trapped more sperm from LF bulls as reported in our previous work [11], which could impede sperm transport to the site of fertilization. In a bovine in vivo study, a moderate influx of PMNs to the endometrium 4 h after insemination resulted in a significantly higher first service conception rate further emphasizing an immune focus on pregnancy rates [20].…”

Section: Discussionsupporting

confidence: 66%

“…In many species to-date, it has been shown that semen (sperm and seminal plasma) is capable of initiating an immune response in the uterus and has been best described in mice, pigs, horses, and more recently in cattle [8][9][10]. Bovine in vitro studies have shown sperm alone can induce a pro-inflammatory response in the uterus [11] mediated by the TLR2/4 pathways [12,13]. In vivo sperm that have come in contact with seminal plasma (SP), but not SP alone, induce transcriptional changes in the uterus after natural mating in cattle [10].…”

Section: Introductionmentioning

confidence: 99%

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The transcriptomic response of bovine uterine tissue is altered in response to sperm from high- and low-fertility bulls

Donnellan

Cormican

Reid³

et al. 2023

Biology of Reproduction

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Despite stringent quality control checks, some bulls with apparently normal semen quality yield lower than expected pregnancy rates. This study profiled the transcriptome and performed histological analysis of the bovine uterus in response to sperm from high fertility (HF) and low fertility (LF) bulls. Post-mortem uterine biopsies and uterine explants were collected from heifers 12 h after a fixed time artificial insemination (AI) to a synchronised oestrus with frozen–thawed semen from 5 HF (fertility rate 4.01% ± 0.25) and 5 LF (fertility rate − 11.29% ± 1.11; mean ± s.e.m.) bulls. Uterine biopsies were also collected from control (CTRL) heifers which were not inseminated. RNA-sequencing and histological analysis were performed for differential gene expression and neutrophil quantification. In the HF treatment relative to CTRL heifers there were 376 genes significantly differentially expressed in the endometrium with just one gene differentially expressed in the LF treatment relative to CTRL heifers. Comparing the HF and LF treatments directly there were 40 significantly differentially expressed genes (DEG; P < 0.05). Transcriptomic analysis shows a predominant role for the inflammatory marker Interleukin-1 alpha, which was further confirmed by immunohistochemistry. Quantification of neutrophils in the endometrium showed a significant effect of sperm, however, there was no difference in neutrophil numbers between HF and LF groups. In conclusion, this novel study clearly shows a distinct inflammatory response to sperm in the endometrium and a divergent transcriptomic response to semen from HF and LFbulls.

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Section: Discussionsupporting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

The transcriptomic response of bovine uterine tissue is altered in response to sperm from high- and low-fertility bulls

Donnellan

Cormican

Reid³

et al. 2023

Biology of Reproduction

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show abstract

“…In addition, Elweza et al [ 39 ] reported that washed frozen–warmed bull spermatozoa induced the expression of pro-inflammatory genes (IL1B, IL8, TNFA and NFkB2) and the same group confirmed that the response was mediated via the TLR2/4 signaling pathway [ 40 ]. Therefore, freezing stimulates the inflammatory-related pathway and apoptotic-related genes, which cause cryo-damage to spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

Cryoprotectants-Free Vitrification and Conventional Freezing of Human Spermatozoa: A Comparative Transcript Profiling

Wang

Todorov

Wang

et al. 2022

IJMS

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Introduction: Spermatozoa cryopreservation is an important technique to preserve fertility for males. This study aimed at exploring the stability of epigenetics information in human spermatozoa, manipulated by two different technologies, freezing and vitrification. Methods: Spermatozoa samples were distributed into three groups: 1. Fresh spermatozoa (control group), 2. Frozen spermatozoa, 3. Vitrified spermatozoa. Epigenetic differences of fresh and cryopreserved spermatozoa were evaluated using high-throughput RNA sequencing. Results: Differentially expressed genes (DEGs) in frozen (1103 genes) and vitrified (333 genes) spermatozoa were evaluated. The bioinformatical analysis identified 8 and 15 significant pathways in groups of frozen and vitrified spermatozoa, respectively. The majority of these pathways are most relevant to immune and infectious diseases. The DEGs of the fertilization process are not detected during vitrification. The freezing process induces more down-regulation of genes and is relevant to apoptosis changes and immune response. Conclusion: Cryopreservation of human spermatozoa is an epigenetically safe method for male fertility preservation. Cryoprotectant-free vitrification can induce more minor biological changes in human spermatozoa, in comparison with conventional freezing.

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Section: Introductionmentioning

confidence: 99%

The transcriptomic response of bovine uterine tissue is altered in response to sperm from high and low fertility bulls

Donnellan

Cormican

Kupisiewicz³

et al. 2020

Animal Reproduction Science

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Comparison of the uterine inflammatory response to frozen-thawed sperm from high and low fertility bulls

Cited by 7 publications

References 35 publications

The transcriptomic response of bovine uterine tissue is altered in response to sperm from high- and low-fertility bulls

The transcriptomic response of bovine uterine tissue is altered in response to sperm from high- and low-fertility bulls

Cryoprotectants-Free Vitrification and Conventional Freezing of Human Spermatozoa: A Comparative Transcript Profiling

The transcriptomic response of bovine uterine tissue is altered in response to sperm from high and low fertility bulls

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