Comparison of the value of measurement of serum galactomannan and Aspergillus-specific antibodies in the diagnosis of canine sino-nasal aspergillosis

Billen, Frédéric; Peeters, Dominique; Peters, Iain R.; Helps, Christopher R; Huynen, Pascale; Mol, Patrick De; Massart, L; Day, M. J.; Clercx, Cécile

doi:10.1016/j.vetmic.2008.07.018

Cited by 34 publications

(30 citation statements)

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“…An indirect ELISA for detection and quantification of Aspergillus-specific IgG antibodies in canine sera using the same aspergillin as for the AGID was modified for use in cats (Billen et al, 2009). Binding activity using polyvinylchloride or polystyrene 96-well plates was assessed as similar.…”

Section: Aspergillus-specific Igg Quantification By Indirect Elisamentioning

confidence: 99%

“…An ELISA to detect a fungal cell wall antigen, galactomannan (GM), in serum (Platelia Aspergillus EIA, Bio-Rad) has a Se of up to 90% in immunocompromised patients, including neutropenic human patients with pulmonary aspergillosis and dogs with disseminated invasive aspergillosis (DIA) (Maertens et al, 1999;Garcia et al, 2012). However, the Se of this test is <30% in nonneutropenic human patients with aspergillosis, in immunocompetent dogs with SNA and in cats with URTA (Billen et al, 2009;Kitasato et al, 2009;Whitney et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Conversely, detection of serum Aspergillus-specific antibodies by agar gel double immunodiffusion (AGID) or by immunoglobulin G (IgG) ELISA has a high test Se in immunocompetent patients, including dogs with SNA (67-88%) and humans with chronic pulmonary aspergillosis (74-94%) (Pomrantz et al, 2007;Billen et al, 2009;Guitard et al, 2012;Ohba et al, 2012). A detectable antibody response is mounted in <30% of neutropenic humans with aspergillosis and dogs with DIA (Day et al, 1985;Hope et al, 2005;Schultz et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Detection of Aspergillus-specific antibodies by agar gel double immunodiffusion and IgG ELISA in feline upper respiratory tract aspergillosis

Barrs

Újvári

Dhand

et al. 2015

The Veterinary Journal

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Aspergillus udagawae (n = 1). 41Brachycephalic purebred cats were significantly more likely to develop URTA than 42 other breeds (P <0.013). The sensitivity (Se) of the AGID was 43% and the specificity (Sp) 43 was 100%. At a cut-off value of 6 ELISA units/mL, the Se of the IgG ELISA was 95.2% and 44 the Sp was 92% and 92.9% for groups 2 and 3 cats, respectively. Aspergillus-specific 45 antibodies against all four cryptic species were detected in one or both assays. Assay Se was

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Section: Aspergillus-specific Igg Quantification By Indirect Elisamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection of Aspergillus-specific antibodies by agar gel double immunodiffusion and IgG ELISA in feline upper respiratory tract aspergillosis

Barrs

Újvári

Dhand

et al. 2015

The Veterinary Journal

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“…Although the AGID is a quite old technique, it has characteristics that justify its practical applicability to the present day, such as easy operation, especially in the handling of small number of samples, low cost and a minimum requirement for laboratory equipment and structure (Lane & Warnock 1977, Poli et al 1981, Billen et al 2009). In addition, being based on a precipitation reaction, the AGID does not require the use of species-specific secondary antibodies, which are required for indirect ELISA and are not commercially available when dealing with wild animals (Graczyk & Cockrem 1995, German et al 2002, Cray et al 2009a, 2009b, Cray 2011, which is the case in our study with penguins.…”

Section: Discussionmentioning

confidence: 99%

Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguins

et al. 2015

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RESUMO.-[Monitoramento sorológico com detecçãode anticorpos para diagnóstico precoce da aspergilose em pinguins em cativeiro.] Este estudo teve como objetivo avaliar a eficácia da detecção de anticorpos anti-Aspergillus fumigatus em pinguins em cativeiro por imunodifusão radial dupla em gel de ágar (IDGA) This study aimed to evaluate the efficacy of detection of anti-Aspergillus fumigatus antibodies in captive penguins by double radial agar gel immunodiffusion (AGID) for the aspergillosis diagnosis. We included 134 Magellanic penguins (Spheniscus magellanicus) in rehabilitation at the Center for Recovery of Marine Animals (CRAM / FURG). All of them were monitored by AGID weekly until its final destination (death or release), totalizing 660 serum samples studied. All animals were clinically accompanied and post-mortem examinations was performed in penguins that died during the studied period. A total of 28% (37/134) of the penguins died, 89.2% (33/37) due to aspergillosis, 11% (4/37) by other causes and 97 were released. From the 33 animals with proven aspergillosis, 21 presented anti-A. fumigatus antibodies by AGID, being the average interval between death and positive AGID 16.4 days. Twelve animals with negative serology died of aspergillosis. The sensitivity and specificity rates were 63.6% and 95% respectively, and the positive and negative predictive values were 80.7% and 88.9% respectively. These data demonstrate that the serological monitoring for detection of antibodies by AGID can be an important tool for the diagnosis of aspergillosis in penguins. o monitoramento sorológico para detecção de anticorpos por IDGA pode ser uma ferramenta importante no diagnós-tico de aspergilose em pinguins.

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“…Moreover, a breed predisposition for German Shepherd dog has been reported, and an inherited immune defect has been speculated in cases of canine aspergillosis 1,3,5,9,14) . In humans, pulmonary aspergillosis is clinically catego- reported that the sensitivity for detection of mycotic antigen is low in serum 4) . Since the lesion of this case was the focal and non-invasive lung disease, no mycotic antigens was considered to be released into the bloodstream.…”

Section: Discussionmentioning

confidence: 99%

A Case of Pulmonary Aspergilloma in a Border Collie

Ito¹,

Yamaya²,

Asano³

et al. 2011

Jpn. J. Vet. Anesth. Surg.

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SUMMARY:A 19-month-old male Border Collie was referred for chronic cough and intermittent hemoptysis. A computed tomography (CT) scan of the chest revealed an air crescent sign, which results in air surrounded by radiopaque material along both its inner and outer margins, in a cavitary lesion in the left caudal lung lobe. Furthermore, a histological section obtained using CT-guided lung biopsy revealed fungal hyphae. Therefore, the animal was diagnosed with canine pulmonary aspergilloma. Antifungal therapy and lobectomy improved the dog's clinical course. Aspergillus fumigatus was isolated from resected specimens and confirmed by performing a histological test.

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Comparison of the value of measurement of serum galactomannan and Aspergillus-specific antibodies in the diagnosis of canine sino-nasal aspergillosis

Cited by 34 publications

References 33 publications

Detection of Aspergillus-specific antibodies by agar gel double immunodiffusion and IgG ELISA in feline upper respiratory tract aspergillosis

Detection of Aspergillus-specific antibodies by agar gel double immunodiffusion and IgG ELISA in feline upper respiratory tract aspergillosis

Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguins

A Case of Pulmonary Aspergilloma in a Border Collie

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