Comparison of the virucidal efficacy of peracetic acid, potassium monopersulphate and sodium hypochlorite on bacteriophages P001 and MS2

Morin, Thierry; Martin, Hélène; Soumet, Christophe; Fresnel, R.; Lamaudière, S.; Sauvage, A.L. Le; Deleurme, Karine; Maris, P.

doi:10.1111/jam.12870

Cited by 22 publications

(14 citation statements)

References 43 publications

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“…Morin et al . [29] reported >4 log reduction for MS2 when exposed to 0.1% NaOCl for 15 minutes on stainless steel carriers. In addition to the longer contact time, the fact that they used larger surface carriers (20x10x1mm) and spread the 10-μL “spill” to cover 70% of the surface before drying for 1 hour, enhancing the impact of drying, might explain their increased inactivation compared with our results.…”

Section: Discussionmentioning

confidence: 99%

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Selection of a Biosafety Level 1 (BSL-1) surrogate to evaluate surface disinfection efficacy in Ebola outbreaks: Comparison of four bacteriophages

Gallandat

Lantagne

2017

PLoS ONE

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The 2014 West African Ebola virus disease outbreak was the largest to date, and conflicting, chlorine-based surface disinfection protocols to interrupt disease transmission were recommended. We identified only one study documenting surface disinfection efficacy against the Ebola virus, showing a >6.6 log reduction after 5-minute exposure to 0.5% sodium hypochlorite (NaOCl) based on small-scale tests (Cook et al. (2015)). In preparation for future extensive, large-scale disinfection efficacy experiments, we replicated the Cook et al. experiment using four potential BSL-1 surrogates selected based on similarities to the Ebola virus: bacteriophages MS2, M13, Phi6, and PR772. Each bacteriophage was exposed to 0.1% and 0.5% NaOCl for 1, 5, and 10 minutes on stainless steel. MS2 and M13 were only reduced by 3.4 log and 3.5 log after a 10-minute exposure to 0.5% NaOCl, and would be overly conservative surrogates. Conversely, PR772 was too easily inactivated for surrogate use, as it was reduced by >4.8 log after only 1-minute exposure to 0.5% NaOCl. Phi6 was slightly more resistant than the Ebola virus, with 4.1 log reduction after a 5-minute exposure and not detected after a 10-minute exposure to 0.5% NaOCl. We therefore recommend Phi6 as a surrogate for evaluating the efficacy of chlorine-based surface disinfectants against the Ebola virus.

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Section: Discussionmentioning

confidence: 99%

“…MS2 is commonly used in environmental studies as a model for enteric viruses and is known to be resistant to several disinfection processes [20,29]. As such, MS2 was expected to be a conservative surrogate for the Ebola virus.…”

Section: Methodsmentioning

confidence: 99%

Selection of a Biosafety Level 1 (BSL-1) surrogate to evaluate surface disinfection efficacy in Ebola outbreaks: Comparison of four bacteriophages

Gallandat

Lantagne

2017

PLoS ONE

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“…Because it is similar to some enteric viruses, the MS2 bacteriophage (an RNA bacteriophage) is the surrogate most commonly proposed and used to assess the efficacy of virucidal decontaminants (D’Souza and Su ; Boudaud et al ). MS2 has been reported to have a sensitivity (D’Souza and Su ; Morin et al ) to selected disinfectants recommended for FMDV decontamination (Krug et al ; Krug et al ). Many methods have been described for detection of MS2 from various inanimate surfaces but few address detections of MS2 from complex environmental matrices (Krug et al ; Krug et al ; Shim et al ).…”

Section: Introductionmentioning

confidence: 99%

The use of bacteriophage MS2 for the development and application of a virucide decontamination test method for porous and heavily soiled surfaces

et al. 2019

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Aims: (i) To develop an analytical method for recovery and quantification of bacteriophage MS2-as a surrogate for foot-and-mouth disease virus-from complex porous surfaces, with and without the presence of laboratorydeveloped agricultural grime; (ii) to evaluate, with a 4-log dynamic range, the virucidal activity of common biocides for their ability to decontaminate surfaces and hence remediate facilities, following a foreign animal disease contamination incident. Methods and Results: An analytical method was developed and optimized for MS2 recovery from simulated agricultural surfaces. The addition of Dey-Engley neutralizing broth to an extraction buffer improved MS2 viability in liquid extracts, with optimal analytical holding times determined as <8 to ≤24 h, depending on matrix. The recovery of MS2 from surface materials decreased in the order: nonporous reference material >grimed porous materials >nongrimed porous materials. In disinfectant testing, two spray applications of pAB were effective against MS2 (≥4-log reduction) on all operational-scale materials. Two per cent citric acid had limited effectiveness, with a ≥4-log reduction observed on a selected subset of grimed concrete samples. Conclusions: Decontamination efficacy test results can be affected by surface characteristics, extraction buffer composition, analytical holding time and surface-specific organism survivability. Efficacy should be evaluated using a test method that reflects the environmental characteristics of the intended application. Significance and Impact of the Study: The results of this study demonstrate the importance of analytical method verification tests for disinfectant testing prior to application in complex environments.

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“…). Previous study with an Stx phage had shown more than 3 log 10 PFU inactivation after the first minute of chlorination at 10 ppm (Allué‐Guardia et al ., ) as well as after 3 min at 5 ppm (Duran et al ., ), and phages λ and MS2 were inactivated at 2500 ppm after 30 min (Halfhide et al ., ), 5000 ppm after 1 min (D'Souza & Su, ) and 4 ppm after 15 min (Morin et al ., ). In contrast, Muniesa and coworkers found that three coliphages, namely a somatic coliphages and two phage infecting O157:H7, were highly resistant to hypochlorite (10 and 20 ppm) because they observed at most ~1 log 10 PFU reductions after a 30‐min incubation (Muniesa et al ., ).…”

Section: Resultsmentioning

confidence: 97%

Resistance of phages lytic to pathogenic Escherichia coli to sanitisers used by the food industry and in home settings

Tomat

Balagué

Aquili

et al. 2017

Int J of Food Sci Tech

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Summary Phages are potentially useful as antimicrobial agents in food‐processing environments, provided they can remain active upon extended storage and in the presence of sanitisers. Survival of six phages lytic against enteropathogenic (EPEC) and shiga‐toxigenic (STEC) Escherichia coli strains was assessed upon storage at 4 °C, −20 °C and −70 °C in phosphate‐buffered‐saline (PBS) and Tris‐magnesium‐gelatine buffer (TMG) for up to 1 year. The phages were also exposed to ethanol, sodium hypochlorite, peracetic acid, quaternary ammonium chloride (biocide A), hydrogen peroxide/peracetic acid/peroctanoic acid (biocide B), p‐toluensulfonchloroamide (biocide D) and alkaline chloride foam (biocide C). Plaque‐forming units remained unchanged when the phages were stored at 4 °C in both buffers tested, but decreased by 3.5 and 5.7 log10 PFU when stored in PBS at −20 and −70 °C, respectively. Moreover, TMG seems to be the most protective suspension medium with decreasing temperature because a 1−log10 PFU reduction was observed at −20 and −70 °C. Incubation in 100% ethanol for 24 h reduced plaque counts only by 2.5 log10 PFU. In 10 ppm of sodium hypochlorite and in biocide B (0.13%), the counts decreased by ~5 and ~6 log10 PFU after 15 min. Finally, biocide A and D reduced counts by 4 and 2–4 log10 PFU after 30 min and 8 h of incubation, respectively. Phages were completely inactivated only by peracetic acid and biocides C and E. Therefore, the phages evaluated might be potentially applied together with classical sanitisers such as ethanol and industrial biocides A, B and D, in disinfection programs against pathogenic STEC and EPEC strains.

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Comparison of the virucidal efficacy of peracetic acid, potassium monopersulphate and sodium hypochlorite on bacteriophages P001 and MS2

Cited by 22 publications

References 43 publications

Selection of a Biosafety Level 1 (BSL-1) surrogate to evaluate surface disinfection efficacy in Ebola outbreaks: Comparison of four bacteriophages

Selection of a Biosafety Level 1 (BSL-1) surrogate to evaluate surface disinfection efficacy in Ebola outbreaks: Comparison of four bacteriophages

The use of bacteriophage MS2 for the development and application of a virucide decontamination test method for porous and heavily soiled surfaces

Resistance of phages lytic to pathogenic Escherichia coli to sanitisers used by the food industry and in home settings

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