Comparison of the Volatile Organic Compounds from Different Biological Specimens for Profiling Potential*

Kusano, Maiko; Mendez, Belacryst; Furton, Kenneth G.

doi:10.1111/j.1556-4029.2012.02215.x

Cited by 69 publications

(53 citation statements)

References 51 publications

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“…CAP of the GC-MS-SPME metabolic profiles allowed the identification of VOCs, which changed in the saliva of T-CD children. Many of the VOCs described in this study corresponded to those previously reported for the saliva of healthy individuals (37,74). These compounds may have various origins, as there are many possible routes of entry into the salivary flow, including environmental exposure through inhalation of air and/or water vapor through the lungs, ingestion through the mouth, food intake, and transdermal absorption through the skin.…”

Section: Discussionsupporting

confidence: 78%

“…These compounds may have various origins, as there are many possible routes of entry into the salivary flow, including environmental exposure through inhalation of air and/or water vapor through the lungs, ingestion through the mouth, food intake, and transdermal absorption through the skin. Moreover, microbial metabolic activities at the level of the oral cavity may also affect the synthesis of VOCs (37,74). First, this study showed some correlations between the microbiome and metabolome of children with CD.…”

Section: Discussionmentioning

confidence: 61%

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Salivary Microbiota and Metabolome Associated with Celiac Disease

Francavilla

Ercolini

Piccolo

et al. 2014

Appl Environ Microbiol

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e This study aimed to investigate the salivary microbiota and metabolome of 13 children with celiac disease (CD) under a glutenfree diet (treated celiac disease [T-CD]). The same number of healthy children (HC) was used as controls. The salivary microbiota was analyzed by an integrated approach using culture-dependent and -independent methods. Metabolome analysis was carried out by gas chromatography-mass spectrometry-solid-phase microextraction. Compared to HC, the number of some cultivable bacterial groups (e.g., total anaerobes) significantly (P < 0.05) differed in the saliva samples of the T-CD children. As shown by community-level catabolic profiles, the highest Shannon's diversity and substrate richness were found in HC. Pyrosequencing data showed the highest richness estimator and diversity index values for HC. Levels of Lachnospiraceae, Gemellaceae, and Streptococcus sanguinis were highest for the T-CD children. Streptococcus thermophilus levels were markedly decreased in T-CD children. The saliva of T-CD children showed the largest amount of Bacteroidetes (e.g., Porphyromonas sp., Porphyromonas endodontalis, and Prevotella nanceiensis), together with the smallest amount of Actinobacteria. T-CD children were also characterized by decreased levels of some Actinomyces species, Atopobium species, and Corynebacterium durum. Rothia mucilaginosa was the only Actinobacteria species found at the highest level in T-CD children. As shown by multivariate statistical analyses, the levels of organic volatile compounds markedly differentiated T-CD children. Some compounds (e.g., ethyl-acetate, nonanal, and 2-hexanone) were found to be associated with T-CD children. Correlations (false discovery rate [FDR], <0.05) were found between the relative abundances of bacteria and some volatile organic compounds (VOCs). The findings of this study indicated that CD is associated with oral dysbiosis that could affect the oral metabolome.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 61%

Salivary Microbiota and Metabolome Associated with Celiac Disease

Francavilla

Ercolini

Piccolo

et al. 2014

Appl Environ Microbiol

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“…RT, retention time; RI, retention index; N, peak number. who processed headspace collection of skin volatiles, found no or very few carboxylic acids in the skin volatiles of axillae (Mebazaa et al, 2011), hands (Zhang et al, 2005;Kusano et al, 2013) or whole body (Logan et al, 2008). Two studies (Ara et al, 2006;Caroprese et al, 2009) reported nine short-chain fatty acids in solvent extracts of foot sweat and in the interdigital area of the foot, respectively.…”

Section: Results and Discussion Chemical Composition Of Foot Volatilesmentioning

confidence: 99%

New methods for field collection of human skin volatiles and perspectives for their application in the chemical ecology of human/pathogen/vector interactions

Dormont

Bessière

McKey

et al. 2013

Journal of Experimental Biology

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SUMMARYOdours emitted by human skin are of great interest to biologists in many fields, with practical applications in forensics, health diagnostic tools and the ecology of blood-sucking insect vectors of human disease. Convenient methods are required for sampling human skin volatiles under field conditions. We experimentally compared four modern methods for sampling skin odours: solvent extraction, headspace solid-phase micro-extraction (SPME), and two new techniques not previously used for the study of mammal volatiles, contact SPME and dynamic headspace with a chromatoprobe design. These methods were tested and compared both on European subjects under laboratory conditions and on young African subjects under field conditions. All four methods permitted effective trapping of skin odours, including the major known human skin volatile compounds. In both laboratory and field experiments, contact SPME, in which the time of collection was restricted to 3min, provided results very similar to those obtained with classical headspace SPME, a method that requires 45min of collection. Chromatoprobe sampling also proved to be very sensitive, rapid and convenient for the collection of human-produced volatiles in natural settings. Both contact SPME and chromatoprobe design may considerably facilitate the study of human skin volatiles under field conditions, opening new possibilities for examining the olfactory cues mediating the host-seeking behaviour of mosquito vectors implicated in the transmission of major diseases.

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“…Helium was used as the carrier gas at 1 mL/min. Using the Varian MS Workstation Software, Version 6.6 (Service Pack 1), all compounds detected were verified and quantitated using an external calibration curve with standards that were purchased from Sigma Aldrich (St. Louis, MO) and recognized as human scent compounds [3,[13][14][15][16][17][18][19]37,40,43,44]. They included: octane; furfural; 2-furanmethanol; nonane; heptanal; 6-methyl-5-hepten-2-one; octanal; benzyl alcohol; (E)-2-octenal; 1-octanol; undecane; octanoic acid methyl ester; 1-nonanol; 2-decanone; dodecane; hexanedioic acid dimethyl ester; tridecane; undecanal; tetradecane; (E)-6,10-dimethyl-5,9-undecadien-2-one (geranyl acetone); pentadecane; and heptadecane.…”

Section: Human Scent Analysis Using Hs-spme/gc-msmentioning

confidence: 99%

“…These skin cells contain volatile organic compounds (VOCs) associated with human scent that consists of various chemical functional groups, such as carboxylic acids, alcohols, aldehydes, aliphatics/aromatics, esters, halides, heterocyclics, ketones, and nitrogen-and sulfur-containing compounds [2,3,[13][14][15][16][17][18][19]. Epithelial skin cells also contain nuclear and mitochondrial deoxyribonucleic acid (nDNA; mtDNA) that can be used to obtain an individual's DNA profile or provide maternal relatedness information.…”

mentioning

confidence: 99%