“…It is also noted that the microbicidal activity of high pressure is enhanced by low pH or temperatures above and below ambient. High hydrostatic pressure acts primarily on non-covalent linkages, such as ionic bonds, hydrogen bonds and hydrophobic interactions, and it promotes reactions in which there is an overall decrease in volume (Adams and Moss, 1995;Indrawati et al, 2008;Sampedro et al, 2008;Chao, et al 2011). It can have profound effects on proteins, where such interactions are critical to structure and function, although the effect is variable and depends on individual protein structure.…”