Comparison of three different C18 HPLC columns with different particle sizes for the optimization of aflatoxins analysis

Comparative study between extraction techniques and column separation for the quantification of sinigrin and total isothiocyanates in mustard seed

“…Limit of detection (LOD) was calculated as three times the standard deviation (SD) and limit of quantification (LOQ) as ten times the SD and relative standard deviation (RSD %) was calculated as the ((SD × 100)/mean) [12]. The height equivalent to a theoretical plate (HETP) was calculated according to Medina and Magan [13].…”

Evaluation of Liquid Chromatographic Behavior of Lumazinic Derivatives, from α-Dicarbonyl Compounds, in Different C18 Columns: Application to Wine Samples Using a Fused-Core Column and Fluorescence Detection

“…29 In the past decade, comparative studies about the separation of compounds in columns with different particle size, such as pharmaceutical compounds 30 or aflatoxins, 29 among others, have been realized by several authors, and in all cases, they concluded that columns with small particle size achieve major separation efficiency and improve the analytical method developed. On the other hand, core−shell columns have been compared with sub-2 μm porous particles columns in pharmaceutical compound analysis, 31 and a higher capacity of the core−shell columns to accomplish better separation efficiency under the same operation conditions was demonstrated.…”

Comparison of five different C18 HPLC analytical columns for the analysis of ochratoxin A in different matrices

“…Medina and Magan [19], found that the retention time of aflatoxins using a Phenomenex Gemini ® C 18 , 3 m were shorter than when using the Phenomenex Luna ® C 18 , 5 m at the same dimensions. However, in this case the chemistry of both columns was very different since the latter columns are used to analyze samples in a wider pH range.…”

“…Recently, it the use of new C 18 packing materials with solid core particles, coated with an outer layer of porous material was shown to improve the quantification of aflatoxins [19], reducing the analysis time without any impact on performance. This has major implications for the reduction in time of a single analysis and may also contribute to significant savings in the use of solvents.…”