Walkway tribometers are used to measure available friction for evaluating walkway safety and pedestrian slip risk. Numerous variables can affect tribometer measurements, including the type and distribution of contaminants on the surface. Here, we quantified the effect of application method on contaminant film thickness, and the effect of film thickness on tribometer measurements on the four reference walkway surfaces used in ASTM F2508-16e. Distilled water, 0.05% sodium lauryl sulfate (SLS) solution, and 0.04% Triton X-100 solution were poured, squirted, and sprayed onto the surfaces to quantify their naturally occurring film thicknesses. These application methods had a significant effect on the resulting film thickness (p < 0.038), with the pour method consistently generating the thickest films and the spray method generating the thinnest films. We then quantified the effect of film thickness for the three contaminants (thickness range 0.3–3.3 mm) on the friction measurements of three common tribometers (Mark IIIB, English XL, and BOT 3000E) on each reference surface. A separate ANOVA was used for each of the 3 × 4 × 3 = 36 combinations of tribometer, surface, and contaminant. Friction measured with the Mark IIIB decreased with increasing film thickness on one surface across all three contaminants and on a second surface with the SLS contaminant. Friction measured with the BOT 3000E was sensitive to film thickness on two surfaces with water and one surface with Triton. The XL was unaffected by contaminant film thickness. Overall, despite significant differences in film thickness with contaminant application method, friction measurements were either insensitive to film thickness or varied only a small amount in all cases except for the Mark IIIB on the roughest surface. Film thickness did not alter the relative slip resistance of the four ASTM F2508 reference surfaces.