Comparison of Three Fecal Steroid Metabolites for Pregnancy Detection Used With Single Sampling in Bighorn Sheep (Ovis Canadensis)

Schoenecker, Kathryn A.; Lyda, Robin O.; Kirkpatrick, Jay F.

doi:10.7589/0090-3558-40.2.273

Cited by 21 publications

(15 citation statements)

References 20 publications

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“…It has also been possible to produce hybrids between Dall sheep (O. dalli) and bighorn sheep (O. canadensis), 20 because they share oestrus cycles of approximately 28 days and a gestation period between 175 and 179 days. [21][22] A recent work on gestations between bighorn and domestic sheep has uncovered that, in these interspecies gestations, there was placental insufficiency, characterized by a significant decrease in the plasma concentration of progesterone and endothelial dysfunction characterized by an increase in plasma levels of vascular endothelial growth factor and placental insulin-like growth factor-1, as well as an overexpression of hypoxia-inducible factor-1α. 23 This work sought to confirm if the production of hybrids is viable, despite the phylogenetic distance between bighorn and domestic sheep, and if the administering of progesterone throughout these gestations could favour placental development and promote foetal growth.…”

Section: Introductionmentioning

confidence: 99%

Progesterone promotes fetal growth in a restricted interspecies gestation (Ovis canadensis x Ovis aries)

Villanueva¹,

Cazadero²,

Mejía³

et al. 2018

Vet Méx OA

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Gestations between bighorn (Ovis canadensis) and domestic sheep (O. aries) can be considered for ex situ conservation of bighorn. In the first experiment, domestic sheep were inseminated with bighorn or domestic semen. Bighorn inseminated sheep showed lower fertility than domestic inseminated sheep (40% vs. 65%, p = 0.11). Bighorn inseminated sheep had longer gestation periods (152.13 days vs. 146.54 days, p < 0.001) and lower progesterone levels during the last third. Hybrid lambs weighed less than domestic lambs (2.46 kg vs. 5.10 kg, p < 0.001). Their placentas were not as long (48.67 cm vs. 72.17 cm, p < 0.001), were less wide (17.83 cm vs. 23.83 cm, p < 0.001), and the weight of cotyledons was lower (1.50 g vs. 3.20 g, p < 0.001). In the second experiment, hybrid embryos (O. canadensis × O. aries) were transferred into domestic recipients, and pregnant ewes were divided into the treated group, which had a progesterone daily dose of 25 mg from weeks 7 to 20, and the non treated group. Gestation in domestic sheep that received one hybrid embryo and progesterone reached 152.60 days, which was similar to the 153.33 days (p = 0.51) in the non treated sheep. Hybrid offspring of the group treated with progesterone were heavier, 3.41 kg, than the control, 2.21 kg (p < 0.001), and their placentas were longer (71.20 vs. 50.83 cm, p = 0.002). Although progesterone levels were lower in domestic females inseminated with bighorn and in the recipients of hybrid embryos, it is possible to establish pregnancies between both species and the birth of viable offspring. The administration of progesterone during gestation increases the length of the placenta and promotes higher birth weights of hybrids. promotes foetal growth in a restricted interspecies gestation (Ovis canadensis × Ovis aries). Veterinaria México OA. 2018;5(3).

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Section: Introductionmentioning

confidence: 99%

Progesterone promotes fetal growth in a restricted interspecies gestation (Ovis canadensis x Ovis aries)

Villanueva¹,

Cazadero²,

Mejía³

et al. 2018

Vet Méx OA

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“…To avoid bias derived from changing water content, most studies analyzing fecal steroids use feces that have been lyophilized before analysis and report the steroid concentrations as nanograms per gram dry feces. However, fresh, wet fecal samples are used increasingly (see Pickard et al 2001;Schoenecker et al 2004;Monclús et al 2009;Pelican et al 2009). Although the dilutive effect of water content on fecal steroid metabolites has been proven (Monclús et al 2009), it has not been demonstrated that bias in results from wet fecal samples not collected daily is due exclusively to water content, or to the microbiological and biochemical processes during the time elapsed until sampling, or a combination of both.…”

Section: Introductionmentioning

confidence: 99%

Effects of fecal age and seasonality on steroid hormone concentration as a reproductive parameter in field studies

2010

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We studied how fecal age (6, 12, 24, 48, 72, and 168 h) and seasonality affect variation in the fecal steroid hormone metabolite concentration in three endangered mammalian species, Mhorr gazelle, Saharan Barbary sheep, and the Iberian lynx. Except for estrogens, concentrations remained stable for at least 48 h in the Mhorr gazelle and the Saharan Barbary sheep. Steroid hormone metabolite concentration remained stable in the Iberian lynx throughout the experiment (1 week). Seasonality was the main factor affecting variation in hormone metabolite concentrations, and although the response was species-and hormone-specific, the dry season resulted in increased hormone metabolite concentrations, while the wet season reduced the concentration.

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“…Sheep are also widely used in research as an experimental model for reproductive studies of wild ruminant artiodactyls and for human neonatology studies (3)(4)(5)(6)(7)(8)(9) . Thus, hormonal monitoring becomes essential for understanding reproductive physiology and monitoring gestational development.…”

Section: Introductionmentioning

confidence: 99%

“…Fecal metabolites of progesterone were evaluated during the estrous cycle and pregnancy in domestic (Ovis aries) (13,14) and wild (Ovis canadensis) (5) ewes, observing a high correlation between fecal metabolites and serum progesterone levels. Additionally, Schoenecker et al (6) also evaluated progesterone and estrone fecal metabolite levels in pregnant ewes, but the hormone levels were not correlated with the serum levels.…”

Section: Introductionmentioning

confidence: 99%

Monitoring of Progesterone and Estrone Fecal Metabolites Throughout Gestation in Ewes

et al. 2019

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The aim of this study was to monitor the progesterone and fecal estrone metabolites throughout gestation in ewes correlating with the serum levels of these steroid hormones. Therefore, fecal and serum samples were collected from 5 weeks before mating and gestation until two weeks postparturition. Serum levels of progesterone and estrone and their fecal metabolites were measured by enzyme immunoassay. Serum and fecal hormonal patterns showed a significant correlation for both hormones (R = 0.8572, P < 0.001 for progesterone and R = 0.5893, P < 0.001 for estrone). The fecal progesterone metabolite levels showed significant increasing values among the three thirds of pregnancies, consistent with the serum levels and with the literature. Additionally, the prepartum peak of estrone in the fecal matrix was identified but without observation in the serum matrix due to the blood collection interval used. Therefore, this study demonstrated the viability of progesterone and estrone monitoring throughout gestation using fecal samples, making noninvasive longitudinal endocrine monitoring throughout gestation possible in this species. ResumoO objetivo deste estudo foi monitorar os níveis de metabólitos fecais de progesterona e estrona ao longo da gestação em ovelhas, correlacionando-os com os níveis séricos desses hormônios esteroides. Assim, amostras de fezes e sangue foram colhidas de cinco ovelhas no período pré-cobertura e durante a gestação, até duas semanas após o parto. Os níveis séricos de progesterona e estrona e de seus metabólitos fecais foram mensurados por enzimaimunoensaio. Os perfis hormonais séricos e fecais apresentaram correlação positiva significativa para os dois hormônios (R = 0,8572, P < 0,001 para progesterona e R = 0,5893, P < 0,001 para estrona). Os níveis de metabólitos fecais de progesterona apresentaram valores significativamente crescente entre os terços da gestação, corroborando com os níveis séricos e com os relatos da literatura. Adicionalmente, foi possível evidenciar o pico pré-parto de estrona na matriz fecal, porém sem registro na matriz sérica, provavelmente devido ao intervalo de coletas aplicado. Deste modo, este estudo demonstrou a AMARAL, R.S. et al.Cienc. anim. bras., Goiânia, v.20, 1-10, e-54208, 2019 viabilidade do monitoramento dos níveis de progesterona e estrona durante a gestação em ovinos utilizando amostras fecais, possibilitando monitoramento endócrino longitudinal não invasivo durante a gestação nessa espécie.

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Comparison of Three Fecal Steroid Metabolites for Pregnancy Detection Used With Single Sampling in Bighorn Sheep (Ovis Canadensis)

Cited by 21 publications

References 20 publications

Progesterone promotes fetal growth in a restricted interspecies gestation (Ovis canadensis x Ovis aries)

Progesterone promotes fetal growth in a restricted interspecies gestation (Ovis canadensis x Ovis aries)

Effects of fecal age and seasonality on steroid hormone concentration as a reproductive parameter in field studies

Monitoring of Progesterone and Estrone Fecal Metabolites Throughout Gestation in Ewes

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