2012
DOI: 10.1111/jpn.12001
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Comparison of three markers for the determination of bacterial protein in terminal ileal digesta in the growing pig

Abstract: The aim of the study was to compare three methods commonly used to determine the concentrations of bacterial protein in digesta collected from the terminal ileum of growing pigs that had been fed a casein-based diet. The amounts of bacterial protein in terminal ileal digesta were determined using three different markers: 2.6-diaminopimelic acid (DAPA) and the d-amino acids, d-aspartic acid (d-Asp) and d-glutamic acid (d-Glu). The effectiveness of each marker was compared against a control based on physical fra… Show more

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Cited by 4 publications
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“…Gram-positive bacteria contain approximately 30-60% peptidoglycan, whereas gram-negative bacteria have only 10% peptidoglycan (Schoenhusen et al 2008). Amino acid composition and structure of peptidoglycans in gram-positive bacteria varies greatly, whereas that of gram-negative bacteria is more consistent (Miner-Williams et al 2013). Knowledge of the DAPA concentration in the walls of bacterial cells is the basis for verification of the hypothesis of the possibility of applying DAPA in the rumen content as a marker of the synthesis of bacterial biomass.…”
mentioning
confidence: 99%
“…Gram-positive bacteria contain approximately 30-60% peptidoglycan, whereas gram-negative bacteria have only 10% peptidoglycan (Schoenhusen et al 2008). Amino acid composition and structure of peptidoglycans in gram-positive bacteria varies greatly, whereas that of gram-negative bacteria is more consistent (Miner-Williams et al 2013). Knowledge of the DAPA concentration in the walls of bacterial cells is the basis for verification of the hypothesis of the possibility of applying DAPA in the rumen content as a marker of the synthesis of bacterial biomass.…”
mentioning
confidence: 99%