Comparison of Three Media for Enumeration of Sclerotia of<i>Macrophomina phaseolina</i>

Cloud, G. L.; Rupe, J. C.

doi:10.1094/pd-75-0771

Cited by 31 publications

(14 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…All plant materials for isolations were surface-disinfested in 1% sodium hypochlorite for 10 min and placed on two different media; modified Peptone PCNB Agar (PPA) medium selective for Fusarium species (25,29) and RB selective medium for M. phaseoUna (10). Isolations were attempted from different areas of the sweetpotato vines including the pencil roots, storage roots, the portion of the vine located below the soil line, and the portion of the vine from the soil line up to 5 cm above the soil line.…”

mentioning

confidence: 99%

Infection of Sweetpotato by Fusarium solani and Macrophomina phaseolina Prior to Harvest

Silva

Clark

2013

Plant Disease

View full text Add to dashboard Cite

da Silva, W. L., and Clark, C. A. 2013. Infection of sweetpotato by Eusarium solani and Macrophomina phaseoiina prior to harvest. Plant Dis. 97:1636-1644.The end rot disease complex, caused mainly by Eusarium soiani and Macrophomina phaseoiina, can be an important postharvest problem in sweetpotato. The disease develops a few weeks after storage roots are harvested and stored. Isolations attempted after harvest showed that the pathogens can be present inside the storage roots before symptoms appear. To determine how and when end rot pathogens enter sweetpotato storage roots, two greenhouse experiments were designed using tissue culture-derived plants free of E soiani and M. phaseoiina. In one experiment, plants were grown in autoclaved soil, and 1 month after transplanting, plants were inoculated at the soil line with either noninfested toothpicks or toothpicks infested with each fungus alone or combined. In the other experiment, plants were grown in noninfested soil or in soil infested with each fungus alone or combined. E solani and M. phaseoiina were isolated from roots, storage roots, and plant stems below the soil line, at the soil line, and 5 cm above the soil line in both experiments. This suggests these fungi are capable of invading sweetpotato plants and storage roots from infested soil, and can systemically colonize the plant from infected plant propagation material.

show abstract

mentioning

confidence: 99%

Infection of Sweetpotato by Fusarium solani and Macrophomina phaseolina Prior to Harvest

Silva

Clark

2013

Plant Disease

View full text Add to dashboard Cite

show abstract

“…Fungal inoculum was produced on corn cob grits, as described by Russin et al (1995). Inoculum density was determined by dilution plating on modiÞed potato dextrose agar (Cloud and Rupe 1991). Fungal inoculum was incorporated into soil by tumbling in a mortar mixer for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Feeding and Maturation by Soybean Looper (Lepidoptera: Noctuidae) Larvae on Soybean Affected by Weed, Fungus, and Nematode Pests

Carter-Wientjes

Russin

Boethel

et al. 2004

View full text Add to dashboard Cite

Feeding and maturation by the soybean looper, Pseudoplusia includens (Walker) (Lepidoptera: Noctuidae), were investigated in a 2-yr study on 'Davis' soybean, Glycine max (L.) Merr., grown alone and combined with the weed hemp sesbania, Sesbania exaltata (Raf.) Rybd. ex. A. W. Hill, the root-knot nematode, Meloidogyne incognita (Kofoid & White) Chitwood, and the charcoal rot fungus, Macrophomina phaseolina (Tassi) Goid. Of the three pests, hemp sesbania had the greatest effects on plant growth and insect feeding and maturation. When fed foliage from soybean stressed by hemp sesbania, soybean looper larvae remained longer in feeding stages, consumed more foliage, and showed altered weight gain compared with larvae fed control foliage. Results suggest that nutrient (s) critical for proper development of larvae may have been limited in weed-stressed soybean foliage. Less dramatic results were observed when larvae fed on foliage from soybean with roots colonized by the charcoal rot fungus. Such larvae consumed more foliage, weighed more, and showed a slight increase in larval feeding period, but only in 1 yr of the study. Colonization of soybean roots by the root-knot nematode had no consistent effects on either the soybean host or insect.

show abstract

“…The morphological differences in Macrophomina are minor with virtually no differences in sclerotial morphology but isolates can be distinguished by differences in pathogenicity. Traditional detection from soil, seeds, diseased roots or the collar region is based on baiting techniques and culture on semiselective media (Cloud & Rupe, 1991). Root colonization can be monitored by estimating colony forming units (CFU) per unit area of root, but this method is labour intensive, difficult to interpret, needs taxonomic expertise and requires at least 5-10 days before results can be analysed.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a polyclonal antibody immunoassay for detection and quantification of Macrophomina phaseolina

Srivastava

Arora

1997

Plant Pathology

View full text Add to dashboard Cite

Detection and quantification of Macrophomina phaseolina, causal agent of charcoal/dry root rot disease in many crop plants, was carried out using the ELISA serological technique. Polyclonal antisera raised to water soluble extracts of mycelium, the residual water insoluble mycelial materials or ribosomal proteins were evaluated for specificity and cross-reactivity with 16 common soil fungi by ODD and DAS-ELISA. Soluble and cell wall antisera exhibited strong cross-reactivity with most of the fungal isolates. Ribosomal antibodies were less reactive to common soil fungi except Fusarium oxysporum f.sp. ciceri. Mycelial antigens of M. phaseolina on chickpea roots were detectable with DAS-ELISA at a minimum concentration of 10 ng g ¹1 at 1 : 100 root : buffer dilution. Quantitative estimation of M. phaseolina on roots was evaluated by ELISA under different temperatures and moisture conditions, and in soil amended with a potential antagonist (Trichoderma harzianum 25-92). A significant reduction in ELISA values was observed in T. harzianumamended treatments. This method may be useful for detection and rapid screening of M. phaseolina under different environmental conditions.

show abstract

Comparison of Three Media for Enumeration of Sclerotia ofMacrophomina phaseolina

Cited by 31 publications

References 0 publications

Infection of Sweetpotato by Fusarium solani and Macrophomina phaseolina Prior to Harvest

Infection of Sweetpotato by Fusarium solani and Macrophomina phaseolina Prior to Harvest

Feeding and Maturation by Soybean Looper (Lepidoptera: Noctuidae) Larvae on Soybean Affected by Weed, Fungus, and Nematode Pests

Evaluation of a polyclonal antibody immunoassay for detection and quantification of Macrophomina phaseolina

Contact Info

Product

Resources

About