Comparison of three methods for 17α-Hydroxyprogesterone

Abstract

We compared the performance of three 17α‐hydroxyprogesterone kits: the double antibody method, the coated tube method (ACTIVE™), both from Diagnostic Systems Laboratories, Inc. (DSL) and the coated tube method (COAT‐A‐COUNT®) from Diagnostic Products Corporation (DPC). The assay performance of the two DSL kits was very similar in terms of sensitivity, intra‐ and inter‐assay precision, linearity of dilution, recovery, and specificity. We also analyzed 190 samples for 17α‐hydroxyprogesterone values using the above three kits. Twenty‐three subjects were from prepubertal population (ages 1 month–13 years), thirty subjects were normal adult males (ages 20–53 years) and the remaining subjects were females in different phase of menstrual cycle (n = 40), on oral contraceptives (n = 20), post‐menopausal (n = 17), or pregnant women in their first, second, or third trimester (n = 60). In addition to these 60 pregnancy samples, we analyzed serial samples from 3 pregnancies. 17α‐OHP levels paralleled the progesterone levels in all three kits. Although there was reasonable correlation between the DPC and the two DSL kits, the 17α‐OHP values were found to be significantly higher with DPC kit during the 2nd and 3rd trimester of pregnancy indicating probable interference in the DPC assay by some structurally related steroids present during pregnancy. The DSL assays may be particularly well suited for measuring 17α‐OHP levels during pregnancy. J. Clin. Lab. Anal. 11:179–185, 1997. © 1997 Wiley‐Liss, Inc.

Rapid screening assay of congenital adrenal hyperplasia by measuring 17α‐hydroxyprogesterone with high‐performance liquid chromatography/electrospray ionization tandem mass spectrometry from dried blood spots

“…Screening for CAH in newborns has become a routine part of many programs by measuring levels of 17αhydroxyprogesterone (17OH-P) (1,2). Currently, neonatal screening procedures for CAH are based on immunoassays (3,4). This approach, while practical, lacks some degree of specificity because of cross-reacting congeners that cannot be separated from 17OH-P in the direct assay (4)(5)(6).…”

Rapid monitoring assay of congenital adrenal hyperplasia with microbore high‐performance liquid chromatography/electrospray ionization tandem mass spectrometry from dried blood spots

“…Monitoring and screening for CAH patients by measuring levels of 17α‐hydroxyprogesterone (17OHP) or other steroids has become a routine part of many programmes 1,. 2 Numerous methods have been described to determine these steroid hormones, such as fluorimetry,3,, 4 radioimmunoassay (RIA),5–8 and high‐performance liquid chromatography (HPLC) 4,. 9,, 10 Most of these, however, are affected by a degree of interference or cross‐reactivity with other steroids.…”

Diagnosis of congenital adrenal hyperplasia by rapid determination of 17α‐hydroxyprogesterone in dried blood spots by gas chromatography/mass spectrometry following microwave‐assisted silylation

“…1)1, 2 has become a routine part of many neonatal screening programs. Currently, neonatal screening procedures for CAH are based on immunoassays 3, 4. This approach, while practical, lacks some degree of specificity because of cross‐reacting congeners that cannot be separated from 17OHP in the direct assay 4–6.…”