Comparison of three microbial bioassay techniques for marine bacteria

Rapid assessment of toxicity of chlorinated aqueous solution by dissolved oxygen depletion and optical density bioassays

“…Da Costa et al ( 26) evaluated the toxicity of secondary effluent disinfected with chlorine on aquatic organisms and found that effluent disinfected with chlorine was toxic for Daphnia similis and Ceriodaphnia silvestrii (cladocerans) and reported that the calculated EC 50 values were 0.04 and 1.28 mg Cl/L, respectively (26). Based on the obtained results, the DOD toxicity assay was more sensitive than the OD toxicity assay so that it showed the toxicity effect at lower toxic concentrations, which is consistent with the results of other studies (17).…”

“…The findings demonstrated that with increasing the chloroform concentration, the time duration for DO concentration depletion in DOD method was increased (Figures 1 and 2). This condition revealed that the duration of responses depending on the toxicant concentration (17,23). The higher concentrations of chloroform caused lower depletion of DO (23).…”

“…In this study, two rapid techniques including DOD and OD bioassays were used to evaluate the chloroform toxicity of aqueous solution according to the methods described by Salama and Salem (17).…”

“…As bacteria are cheap to cultivate, grow rapidly, technically simple, require low space compared with fish bioassay, available and contain enzymatic and physiological processes, also found in larger organisms, they are good bioassay tools. Bacteria respond more than most other organisms to change their condition and sensitive to toxicity compound (17).…”

“…To evaluate the toxicity of linden, mercury, and wastewater, dissolved oxygen depletion (DOD) and optical density (OD) bioassays were used. It was revealed that DOD and OD bioassays can evaluate the toxicity of the above-mentioned contaminants (17). Conventional techniques were precise but expensive, and need special equipment and trained personnel (16).…”