Comparison of Three Nucleic Acid Amplification Tests and Culture for Detection of Group B
            <i>Streptococcus</i>
            from Enrichment Broth

Shin, Ji Hyun; Pride, David T.

doi:10.1128/jcm.01958-18

Cited by 27 publications

(22 citation statements)

References 34 publications

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“…Rapid nucleic acid amplification tests (PCR) performed intrapartum are increasingly finding their way into clinical settings, and several studies have concluded that these screening assays perform well [8,9].…”

Section: Introductionmentioning

confidence: 99%

A comparison of GenomEra® GBS PCR and GeneXpert ® GBS PCR assays with culture of GBS performed with and without broth pre-enrichment

Nielsen

Frimodt‐Møller

Khalil

2020

Eur J Clin Microbiol Infect Dis

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This study was designed to compare the performance of GeneXpert® and GenomEra® group B streptococcus (GBS) PCR assays, held up against standard culture of GBS performed with and without broth pre-enrichment. In Denmark, the strategy for preventing early onset GBS infection (EOGBS) is risk factor based. Three hundred and sixty six women fulfilling one or more of the criteria for presence of risk factors for EOGBS were prospectively included. Rectovaginal swab samples were taken intrapartum and tested bed-site by the GenomEra® and the GeneXpert® GBS PCR assays and cultured at the microbiology laboratory using Granada agar plates with and without prior growth of sampling material in selective enrichment broth. Among 366 participants tested intrapartum, 99 were GBS-positive by culture, 95 by GenomEra, and 95 by GeneXpert. Compared with culture, the GenomEra and the GeneXpert performed with a sensitivity of 91.8% and 91.7% and a specificity of 98.1% and 97.3%, respectively. A combined reference standard was established by defining true positives as either culture-positive samples or culture-negative samples where both the GeneXpert and the GenomEra GBS PCR assays were positive. Using this, the sensitivity increased to 92.2% and the specificity to 99.6% for GenomEra and to 92.0% and 96.8% for GeneXpert. The use of selective broth enrichment found only three additional GBS culture-positive samples. The performance of the two PCR methods examined was very similar and close to the findings by culture, and both PCR assays are thus applicable as rapid intrapartum bed-site tests.

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Section: Introductionmentioning

confidence: 99%

A comparison of GenomEra® GBS PCR and GeneXpert ® GBS PCR assays with culture of GBS performed with and without broth pre-enrichment

Nielsen

Frimodt‐Møller

Khalil

2020

Eur J Clin Microbiol Infect Dis

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“…Use of selective or chromogenic medium has increased the sensitivity of culture for GBS, but these methods may still be less sensitive than nucleic acid amplification tests (NAATs) for the detection of GBS ( 5 – 8 ). There are several commercially available NAATs for the detection of GBS that increase sensitivity, require less hands-on time, and provide a faster result, but they are often more expensive and may have slightly lower specificity than culture ( 9 , 10 ).…”

Section: Introductionmentioning

confidence: 99%

Digital Image Analysis for the Detection of Group B Streptococcus from ChromID Strepto B Medium Using PhenoMatrix Algorithms

et al. 2020

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Group B Streptococcus (GBS) can be found to colonize about 25% of all healthy, adult women and is the leading infectious cause of early neonatal morbidity and mortality in the United States. This study evaluates the clinical performance of PhenoMatrix (PM) Chromogenic Detection Module (CDM) digital imaging software in detection of GBS from LIM broth plated on CHROMID® Strepto B Chromogenic media (ChromID) using the WASP automated processor. The performance of the PM CDM was compared to manual culture review of the digital images and molecular detection of GBS. ChromID alone had a sensitivity and specificity of 84.5% and 94.7%, respectively, after 48 hours as compared to nucleic acid amplification testing (NAAT). As compared to the composite reference for positivity, when PM CDM was used to detect GBS from ChromID the sensitivity was 100% with no true positive GBS isolates missed by 48 hours of incubation. Overall, evaluating all three methods for the detection of GBS, the sensitivities of NAAT, ChromID plus PM CDM at 48 hours, and ChromID alone at 48 hours were 96.8%, 95.5% and 90.3%, respectively. The specificities of NAAT, ChromID plus PM CDM and ChromID alone were 97.7%, 63.0%, and 95.4%, respectively. The use of ChromGBS in combination with the PM CDM was similar to the sensitivity of molecular detection. Further, the algorithm never called a culture negative that was determined to be positive by manual reading and identified an additional eight true positive specimens that were missed by manual digital image culture reading.

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“…also found that the sensitivity and specificity of GeneXpert ® and GenomEra ® PCR assays were 92.2% and 99.6%, and 92% and 96.8%, respectively ( Nielsen et al., 2020 ). NAATs were proved to be more sensitive than the post-enrichment subculture as demonstrated by the identification of GBS that were positive by NAATs assay but negative by the post-enrichment subculture method ( Buchan et al., 2015 ; Shin and Pride, 2019 ). In conclusion, NAATs offer faster time to result, greater throughput, and potentially greater sensitivity, and they are suggested as an alternative for GBS screening in pregnant women ( Rosa-Fraile and Spellerberg, 2017 ; Guo et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Diagnostic Performance of Various Methodologies for Group B Streptococcus Screening in Pregnant Woman in China

Gao

Deng

Huang

et al. 2021

Front. Cell. Infect. Microbiol.

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Maternal vaginal/rectal colonization of group B streptococcus (GBS) is a main risk for neonatal invasive infection. Efficient determination of GBS colonization in pregnant women is crucial. This study aimed to investigate the prevalence of GBS carriage and evaluate the diagnostic performance of six methodologies for GBS screening conducted in China, including blood agar plate, liquid chromogenic medium, and loop-mediated isothermal amplification (LAMP) without pre-enrichment, chromogenic agar plate with pre-enrichment, and GBS antigen detection without and with pre-enrichment in comparison with the standard reference method (Lim broth-enriched subculture with plating on 5% sheep blood agar). Vaginal/rectal swabs were collected from 1,281 pregnant women at 35–37 weeks of gestation. Of them, 309 were taken in triplicate, one for Lim broth-enriched subculture, one for blood agar plate, and the third for GBS antigen detection (Reagent W); 177 were acquired in duplicate, one for Lim broth-enriched subculture and the other for GBS antigen detection (Reagent H); 502 were obtained in duplicate, one for Lim broth-enriched subculture and the other for liquid chromogenic medium; 158 were collected in duplicate, one for Lim broth-enriched subculture and the other for LAMP; and 135 were inoculated in Lim broth-enriched for GBS antigen detection (Reagent W) and subculture with chromogenic agar plate and 5% blood agar plate. The overall prevalence of GBS carriage was 10.1% (130/1,281, 95% CI: 8.5–12.1%) according to the standard reference method. Compared with the standard reference method, the LAMP had excellent performance of sensitivity (100%, 95%CI: 83.4–100%), specificity (94%, 95%CI: 88.1–97.1%), and Yoden index (0.940); as well as the blood agar plate with sensitivity (81.5%, 95%CI: 61.3–93.0%), specificity (100%, 95%CI: 98.3–100.0%), and Yoden index (0.815). The other four methods were not sufficient to reach the threshold in terms of sensitivity or specificity compared to the standard reference method. Furthermore, for LAMP, results can be obtained within 0.5–1 h, while for blood agar plate, which needed 24–48 h, and further identification was required. Our data suggested that the performance of LAMP was highly comparable to the standard Lim broth-enriched subculture and LAMP is considered as an alternative for fast and accurate GBS screening.

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Comparison of Three Nucleic Acid Amplification Tests and Culture for Detection of Group B Streptococcus from Enrichment Broth

Cited by 27 publications

References 34 publications

A comparison of GenomEra® GBS PCR and GeneXpert ® GBS PCR assays with culture of GBS performed with and without broth pre-enrichment

A comparison of GenomEra® GBS PCR and GeneXpert ® GBS PCR assays with culture of GBS performed with and without broth pre-enrichment

Digital Image Analysis for the Detection of Group B Streptococcus from ChromID Strepto B Medium Using PhenoMatrix Algorithms

Diagnostic Performance of Various Methodologies for Group B Streptococcus Screening in Pregnant Woman in China

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