“…The dietary concentrations of Sc are based on CFU values detected at the moment of the start of the trial. The base diet -better detailed in Trevisi et al (2015a) was based on wheat shorts, corn, barley, extruded barley, soybean meal 50, whey dehydrated and skimmed, potato, protein concentrate, vegetable oil, dehydrated beet pulp, calcium carbonate, monosodium phosphate hydrated, sodium chloride, vitamin and trace mineral mixture, and was integrated with 0.4% L-lysine HCl, 0.2% DL-methionine, 0.15% L-threonine, 0.07% L-tryptophan. The colistin (Sintofarm, Guastalla, Reggio Emilia, Italy) treatment was used as a positive control owing to its activity against the ETEC strain used for the challenge, following a procedure frequently used for this purpose in other trials involving an ETEC challenge (Trevisi et al, 2015b).…”