Comparison of three potato leafroll virus antisera and a single Beet western yellows virus antiserum for luteovirus detection in potato

Gallenberg, Dale J.; Zitter, Thomas A.; Jones, Edward

doi:10.1007/bf02854206

Cited by 2 publications

(1 citation statement)

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“…Samples that tested negative for PLRV by ELISA might be infected with other viruses or pathogens. There is evidence to indicate that other viruses can infect and produce leafroll‐type symptoms in potato; for example, isolates of Beet western yellow virus (BWYV) alone or in combination with PLRV isolates, can induce typical severe leafroll symptoms in potato plants and are responsible for the potato leafroll disease complex (Duffus, 1981a,b; Gallenberg et al., 1987). BWYV was detected in potato leafroll samples from nine states and provinces in North America and the BWYV‐positive samples represented 40% in 1983 and 62.5% in 1984 of the total number of samples tested (Gallenberg et al., 1987).…”

Section: Resultsmentioning

confidence: 99%

Incidence and Molecular Analysis ofPotato leafroll virusin Iran

Pooramini

Heydarnejad

Massumi

2010

Journal of Phytopathology

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Potato leafroll virus (PLRV) is one of the most prevalent potato viruses in Iran. This report describes the distribution of PLRV in four Provinces from southeastern, southern, north-eastern and north-western Iran and phylogenic relationships of Iranian PLRV to other previously reported PLRV isolates. PLRV was detected in c.15% (126 of 836) of symptomatic potato samples (showing yellowing and leaf roll) by double antibody sandwich enzyme-linked immunosorbent assay. The coat protein (CP) gene of four isolates was amplified by reverse transcription-polymerase chain reaction using specific primers. The nucleotide sequence showed a high degree of sequence identities between all PLRV isolates. Three of the four Iranian isolates were 100% identical at the amino acid level (for the domain sequenced), but the fourth isolate differed by an amino acid. For isolate PLRV-Ke, we amplified the open reading frame (ORF0) (which overlaps the 5¢ end of the ORF1) and the sequence analysis indicated that the amino acid sequences of the ORF0 and the 5¢ end of the ORF1 showed identity of 92.7-100% and 90.2-99% with that of the GenBank PLRV isolates, respectively. In contrast to the amino acid sequence of the CP, a constructed phylogenetic tree using the amino acid sequence of the ORF0 differentiated the Iranian isolate (PLRV-Ke) from some European and African isolates.

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Section: Resultsmentioning

confidence: 99%