Comparison of three screening methods to select mixed-microbial inoculum for mixed-acid fermentations

Golub, Kristina W.; Forrest, Andrea K.; Wales, Melinda E.; Hammett, Amy Jo M.; Cope, Julia; Wilkinson, Heather H.; Holtzapple, Mark T.

doi:10.1016/j.biortech.2012.10.010

Bioresource Technology

2013

DOI: 10.1016/j.biortech.2012.10.010

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Comparison of three screening methods to select mixed-microbial inoculum for mixed-acid fermentations

Kristina W. Golub

Andrea K. Forrest

Melinda E. Wales

et al.

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Cited by 3 publications

References 25 publications

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Assessment of Shock Pretreatment of Corn Stover Using the Carboxylate Platform

Darvekar

Holtzapple

2015

Appl Biochem Biotechnol

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Corn stover was pretreated with lime and shock, a mechanical process that uses a shockwave to alter the biomass structure. Two pretreatments (lime-only and lime + shock) were evaluated using enzymatic hydrolysis, batch mixed-culture fermentations, and continuous countercurrent mixed-culture fermentation. In a 120-h enzymatic hydrolysis, shock pretreatment increased the glucan digestibility of submerged lime pretreatment (SLP) corn stover by 3.5 % and oxidative lime pretreatment (OLP) corn stover by 2.5 %. The continuum particle distribution model (CPDM) was used to simulate a four-stage continuous countercurrent mixed-culture fermentation using empirical rate models obtained from simple batch experiments. The CPDM model determined that lime + shock pretreatment increased the total carboxylic acids yield by 28.5 % over lime-only pretreatment in a countercurrent fermentation with a volatile solids loading rate (VSLR) of 12 g/(L/day) and liquid retention time (LRT) of 30 days. In a semi-continuous countercurrent fermentation performed in the laboratory for 112 days with a VSLR of 1.875 g/(L day) and LRT of 16 days, lime + shock pretreatment increased the total carboxylic acid yield by 14.8 %. The experimental results matched closely with CPDM model predictions (4.05 % error).

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Assessment of Shock Pretreatment of Corn Stover Using the Carboxylate Platform

Darvekar

Holtzapple

2015

Appl Biochem Biotechnol

Self Cite

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Converting wastewater sludge and lime-treated sugarcane bagasse to mixed carboxylic acids – a potential pathway to ethanol biofuel production

Rughoonundun

Holtzapple

2017

Biomass and Bioenergy

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Microbial communities for valorizing biomass using the carboxylate platform to produce volatile fatty acids: A review

Holtzapple

Weimer

et al. 2022

Bioresource Technology

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Comparison of three screening methods to select mixed-microbial inoculum for mixed-acid fermentations

Cited by 3 publications

References 25 publications

Assessment of Shock Pretreatment of Corn Stover Using the Carboxylate Platform

Assessment of Shock Pretreatment of Corn Stover Using the Carboxylate Platform

Converting wastewater sludge and lime-treated sugarcane bagasse to mixed carboxylic acids – a potential pathway to ethanol biofuel production

Microbial communities for valorizing biomass using the carboxylate platform to produce volatile fatty acids: A review

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