Comparison of three strip-type tests and two laboratory methods for salivary buffering analysis

Abstract

This study evaluated the correlation between three strip-type, colorimetric tests and two laboratory methods with respect to the analysis of salivary buffering. The strip-type tests were saliva-check buffer, Dentobuff strip and CRT Ò Buffer test. The laboratory methods included Ericsson's laboratory method and a monotone acid/base titration to create a reference scale for the salivary titratable acidity. Additionally, defined buffer solutions were prepared and tested to simulate the carbonate, phosphate and protein buffer systems of saliva. The correlation between the methods was analysed by the Spearman's rank test. Disagreement was detected between buffering capacity values obtained with three strip-type tests that was more pronounced in case of saliva samples with medium and low buffering capacities. All striptype tests were able to assign the hydrogencarbonate, di-hydrogenphosphate and 0.1% protein buffer solutions to the correct buffer categories. However, at 0.6% total protein concentrations, none of the test systems worked accurately. Improvements are necessary for strip-type tests because of certain disagreement with the Ericsson's laboratory method and dependence on the protein content of saliva.

Efficacy of pastes containing CPP-ACP and CPP-ACFP in patients with Sjögren’s syndrome

“…d shows remineralized enamel surface with amorphous deposits of mineralized material-calcium fluoride less saliva for the volume of acid in the strip that may influence the final colour [46,48]. When evaluating the accuracy of the strip-type tests for salivary buffering analysis, Cheaib et al [46] reported a 0.95 correlation of the strip test used in the present study and the Ericson's laboratorial method [49] which is considered a golden standard to assess the buffering capacity of the saliva. On the other hand, Kitasako et al [48] reported that colorimetric tests tend to underestimate the buffering capacity.…”

“…Therefore, in the present study, we have determined the salivary pH and buffering capacity immediately after the proposed contact time of the colour-changing strip and the saliva to avoid potential misreading of the results. Although it seems easy, colour matching of the strip with the colour supplied scheme can be influenced by the subjectivity of an examiner [46]. Individual colour perception can be affected by colour vision deficiencies, ambient lighting and the operator's experience [47].…”

Changes in Salivary Flow Rate and Buffering Capacity in Children with Newly Diagnosed Type 1 Diabetes Mellitus

“…Strip üzerin-deki skalanın renk ayrımına göre kolorimetrik yön-temle, tükürük tamponlama kapasitesi hakkında bilgi sahibi olunmuştur. 18 …”

Assessment of dental caries risk in children by color Doppler ultrasonography by the evaluation of changes in blood perfusion in the salivary glands during salivary stimulation

“…In this regard, the knowledge of normal salivary composition, flow, and functional properties may permit a better assessment of dental caries susceptibility. Some studies have seemed to support the validity of a combined evaluation of the decayed, missing, and filled teeth (DMFT) index, pH, salivary flow, and buffer power of saliva in the prediction of caries (4,7,8). However, salivary flow rate (SFR) is undoubtedly the most important single parameter because the cariostatic activity or efficacy of practically all other salivary parameters depends on the flow rate (9).…”

“…It is generally accepted that whole saliva, secreted by the major and minor salivary glands, and its components are critical for the maintenance of the integrity of the teeth and oral soft tissues (4). The main salivary parameters associated with an increased risk for caries are buffer capacity, cariogenic bacteria counts, pH, flow rate, and total protein content (5,6).…”