26This study aimed to study the co-activation of hamstring-quadriceps muscles 27 during submaximal strength exercises without the use of maximum voluntary isometric 28 contraction testing and compare (i) the inter-limb differences in muscle activation, (iithe 29 intra-muscular group activation pattern, and (iii) the activation during different phases of 30 the exercise. Muscle activation was recorded by surface electromyography of 19 elite 31 male youth players. Participants performed five repetitions of the Bulgarian squat, lunge 32 and the squat with an external load of 10 kg. Electrical activity was recorded for the rectus 33 femoris, vastus medialis, vastus lateralis, biceps femoris and semitendinosus. No 34 significant inter-limb differences were found (F 1, 13 =619; p=0.82; partial η 2 =0.045). 35 Significant differences were found in the muscle activation between different muscles 36 within the muscle group (quadriceps and hamstrings) for each of the exercises : Bulgarian 37 squat (F 1,18 =331: p<0.001; partial η 2 =0.80), lunge (F 4,72 =114.5; p<0.001; partial η 2 =0.86) 38 and squat (F 1,16 =247.31; p<0.001; partial η 2 =0.93).Differences were found between the 39 concentric, isometric and eccentric phases of each of the exercises (F 2, 26 =52.27; p=0.02;40 partial η 2 =0.80). The existence of an activation pattern of each of the muscles in the three 41 proposed exercises could be used for muscle assessment and as a tool for injury recovery.
Introduction
43Performance in soccer depends on psychological, physiological and 44 biomechanical factors (1, 2). The study of these factors not only helps improve 45 performance, but also works towards injury prevention. Performance and injury 46 prevention are not isolated fields, and the presence of an injury can affect on-field 47 performance. Measurement of the muscular capacity of players is an important factor in 48 the evaluation and prediction of the functional capacity of the player (3).
49Muscular capacity has been traditionally measured using isokinetic machines (4, 50 5). However, recently many studies have used electromyography (EMG) (6-8). One of 51 the major aims of such studies has been to to determine asymmetry in soccer players, 52 which can affect both performance (9, 10) and injury (11). Such comparative studies have 53 been carried out in youth soccer players as well (12-15) finding that there may be a 54 neuromuscular pattern and that force work could be related to a reduction in the injury 55 rate in football players. 56 Asymmetry in soccer players could be offset with proper exercise prescription, 57 which facilitates improvements in musculoskeletal function by addressing the specific 58 needs of the subject as an integral part of any rehabilitative, preventive, or maintenance 59 program (16). Functional weight-bearing exercises have received a significant amount of 60 attention as the preferred mode of exercise for lower extremity strengthening (17). In 61 studies with EMG, basic strength exercises (forward lunge, bugarian squat, lateral ste...