Comparison of time-resolved fluoroimmunoassay for clenbuterol residues in pig liver with enzyme-linked immunosorbent assay

Abstract

A time-resolved fl uoroimmunoassay (TR-FIA) for determination of clenbuterol (CLB) in pig liver was developed. The limit of detection (LOD) was 0.02 ng g -1 and limit of quantifi cation (LOQ), 0.08 ng g -1 . Recoveries ranged from 89.3 to 117.9% for pig liver at spiked levels of 0.1-5 ng g -1 . The results obtained by the TR-FIA and enzyme-linked immunosorbent assay (ELISA) showed a good correlation. The established TR-FIA was applied for screening pig liver from the local market and confi rmed by gas chromatography-mass spectrometry (GC-MS). This proposed technique could be used for routine screening for drug residues.

Selenium speciation in different organs of African catfish (Clarias gariepinus) enriched through a selenium-enriched garlic based diet

“…5,6 The most important and successful nutritional supplement is still selenized yeast although vegetables, 7,8 bread 9 and biscuits 10 are promising alternatives. [11][12][13] The literature regarding selenium speciation in other foodstuffs, for instance those of animal origin, is less abundant, 14 but recently several articles have been published on this subject, 15 for example studies in lamb, chicken, 16 cows, calves 17 and several fish species, [18][19][20] usually enriched by a Se-fortified diet. However, current opinions of the EFSA (European Food Safety Authority) indicate that there is insufficient data about the safety of selenium compounds (as selenomethylselenocysteine) in food supplements.…”