Comparison of ‘time to detection’ values between BacT/ALERT VIRTUO and BacT/ALERT 3D instruments for clinical blood culture samples

Congestrì, Francesco; Pedna, Maria Federica; Fantini, Manuela; Samuelli, Michela; Schiavone, Pasqua; Torri, Arianna; Bertini, S.; Sambri, Vittorio

doi:10.1016/j.ijid.2017.06.012

Cited by 16 publications

(10 citation statements)

References 15 publications

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“…Unfortunately, we did not include less frequent but clinically relevant species (i.e., Streptococcus bovis group species or other alpha-hemolytic streptococcal species) in our study, as we restricted the list of evaluable microorganisms to the groups of species commonly causing BSIs. Accordingly, coagulase-negative staphylococci, E. coli , Enterobacteriaceae (other than E. coli ), S. aureus, Enterococcus species, viridans group streptococci, P. aeruginosa , and Candida species were the focus of clinical evaluations of the VIRTUO system (Congestrì et al, 2017; Jacobs et al, 2017). In this sense, the simulated BCs proposed by us continue to be a good surrogate of clinical BCs, particularly when an accurate assessment of new advanced diagnostics is required (Beyda et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

In vitro Evaluation of BACT/ALERT® VIRTUO®, BACT/ALERT 3D®, and BACTEC™ FX Automated Blood Culture Systems for Detection of Microbial Pathogens Using Simulated Human Blood Samples

et al. 2019

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Blood culture (BC) is still the standard for diagnosing bloodstream infections (BSIs), especially those caused by bacteria and fungi. Infection-complicating sepsis or septic shock often occurs at BSI onset, making necessary to improve the diagnostic yield of positive BCs. Among the BC systems currently available, the BACT/ALERT® VIRTUO® (VIRTUO) system has been developed to shorten time to detection (TTD) of positive BCs. In this study, we assessed TTD for 330 clinically relevant species including 14 Gram-positive, 14 Gram-negative, and 5 yeast isolates in spiked human blood samples that were tested in parallel with VIRTUO BACT/ALERT® 3D (BTA3D) and BACTEC™ FX (BACTEC) systems. We inoculated 30 colony-forming unit (CFU) from each microbial suspension into BACT/ALERT® Plus or BACTEC™ Plus (aerobic/anaerobic or pediatric) BC bottles, and we used two different blood volumes to simulate, respectively, the BCs collected from adult and pediatric patients. Of 2,610 bottles tested, 2,600 (99.6%) signaled positive in the three systems. Only the BACTEC system did not detect Staphylococcus lugdunensis isolates in anaerobic bottles. Among adult simulated cultures, the median TTD was significantly shorter for aerobic/anaerobic bottles incubated in VIRTUO (11.6 h and 10.1 h) compared to bottles incubated in either BTA3D (13.3 and 12.3 h) or BACTEC (13.5 and 12.2 h) system. Among pediatric simulated cultures, the median TTD was significantly shorter for bottles incubated in VIRTUO (11.2 h) compared to bottles incubated in either the BTA3D (13.0 h) or BACTEC (12.5 h) system. Compared to BTA3D and/or BACTEC systems, VIRTUO allowed faster growth detection for most of the 33 microbial species tested. Notable examples were Salmonella spp. (7.4 h by VIRTUO vs. 10.1 h and 9.2 h by either BTA3D or BACTEC) and Streptococcus agalactiae (8.1 h by VIRTUO vs. 10.3 and 9.4 h by either BTA3D or BACTEC). The few notable exceptions included Stenotrophomonas maltophilia and some Candida species. Together, these findings confirm that VIRTUO has greater potential of improving the laboratory detection of bacteremia and fungemia than the progenitor BTA3D or the competitor BACTEC system.

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Section: Discussionmentioning

confidence: 99%

In vitro Evaluation of BACT/ALERT® VIRTUO®, BACT/ALERT 3D®, and BACTEC™ FX Automated Blood Culture Systems for Detection of Microbial Pathogens Using Simulated Human Blood Samples

et al. 2019

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“…Further, a retrospective study comparing the TTD between two different periods showed that the cumulative percentage was shorter for common pathogens using Virtuo than using 3D [14]; however, the method of measuring the TTD was not clearly described. In addition, this was not a head-to-head comparison.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Clinical Performance Between BacT/Alert Virtuo and BacT/Alert 3D Blood Culture Systems

et al. 2019

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Background BacT/Alert Virtuo (BioMérieux, Durham, NC, USA) is a recently developed blood culture system that includes functions of automatic registration, loading, and unloading of the blood culture bottles, as well as measurement of blood volume. We compared the performances between the BacT/Alert Virtuo and 3D (BioMérieux) blood culture systems. Methods A total of 952 patients (1,904 sets) visiting an university-affiliated hospital in Korea for blood cultures were enrolled. Five milliliters of blood was added into each of the two aerobic (FA Plus) and two anaerobic (FN Plus) bottles of the Virtuo and 3D systems for a single set. Positive rate and time to detection (TTD) were compared between the two systems. Results The positive rates were 8.3% and 8.4% in FA Plus bottles and 7.8% and 8.3% in FN Plus bottles, in the Virtuo and 3D systems, respectively ( P >0.05). Median TTDs were shorter in the Virtuo than in the 3D system for all isolates (11.5 hours [N=305] vs 11.8 hours [N=318], P <0.001), Staphylococcus aureus (N=38; 14.3 hours vs 16.0 hours, P =0.021), and Escherichia coli (N=117; 10.4 hours vs 11.0 hours, P <0.001). Conclusions The Virtuo has the potential to detect pathogens early in all bottle types. This might improve the prognosis of sepsis by allowing for implementation of expeditious management.

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“…Each set consists of an aerobic bottle (BacT/ALERT ® FA Plus, bioMérieux, Marcy l’Etoile, France) and an anaerobic lytic bottle (BacT/ALERT ® FN Plus, bioMérieux), containing adsorbent polymeric beads for the neutralization of antimicrobial agents, which are inoculated with 10 mL of blood each. When samples arrived at the Laboratory, the two sets of blood cultures were incubated in a BacT/ALERT (bioMérieux) automated instrument for up to 5 days [ 13 ]; positive samples were identified through time-of-flight mass spectrometry coupled with matrix-assisted laser desorption/ionization (MALDI-TOF) performed on Vitek MS (bioMérieux) instrument. The susceptibility testing was achieved using the automated Vitek 2 (bioMérieux) system from positive blood culture bottles after performing a Gram stain and a concentration procedure [ 11 – 13 ].…”

Section: Methodsmentioning

confidence: 99%

“…Although many infections can be detected after 24 to 48 hours, it may take up to 5-days incubations to capture the majority of slow-growing bacteria and fungi associated with BSI, and the antibiotic susceptibility determination require an additional 6 to 24 h [ 10 – 13 ].…”

Section: Introductionmentioning

confidence: 99%

Rapid diagnosis of bloodstream infections in the critically ill: Evaluation of the broad-range PCR/ESI-MS technology

et al. 2018

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Bloodstream infection (BSI) and associated sepsis represent a major source of mortality in industrialized countries. Prompt treatment with targeted antibiotics affects both the financial impact and the clinical outcome of BSI: every hour gained in initiating the correct antimicrobial therapy significantly increases the probability of patient survival. However, the current standard-of-care, which depends on blood culture-based diagnosis, are often unable to provide such a fast response. Fast and sensitive molecular techniques for the detection of sepsis-related pathogens from primary blood samples are strongly needed. The aim of this study was to assess the usefulness of the IRIDICA BAC BSI Assay, a PCR/ESI-MS-based technology for the early diagnosis of bloodstream infections from primary blood samples in critical patients. This evaluation has been performed by comparison with the traditional culture-based methods. The study was performed on a total of 300 prospective whole blood specimens obtained from patients suspected of sepsis, admitted to enrolling ER units from The Greater Romagna Area. The overall concordance between the two techniques was of 86%, with a calculated sensitivity of 76% and an assay specificity of 90%. The clinical significance of discrepant results was evaluated reviewing the patients’ clinical records and the results of additional relevant microbiological tests. The data here obtained support the ability of the IRIDICA BAC BSI Assay to identify a broad range of bacteria directly from primary whole blood samples, within eight hours. This might allow a timely administration of a suitable treatment.

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Comparison of ‘time to detection’ values between BacT/ALERT VIRTUO and BacT/ALERT 3D instruments for clinical blood culture samples

Abstract: The new VIRTUO blood culture system can reduce the TTD for more than 75% of isolated microorganisms.

Cited by 16 publications

References 15 publications

In vitro Evaluation of BACT/ALERT® VIRTUO®, BACT/ALERT 3D®, and BACTEC™ FX Automated Blood Culture Systems for Detection of Microbial Pathogens Using Simulated Human Blood Samples

In vitro Evaluation of BACT/ALERT® VIRTUO®, BACT/ALERT 3D®, and BACTEC™ FX Automated Blood Culture Systems for Detection of Microbial Pathogens Using Simulated Human Blood Samples

Comparison of Clinical Performance Between BacT/Alert Virtuo and BacT/Alert 3D Blood Culture Systems

Rapid diagnosis of bloodstream infections in the critically ill: Evaluation of the broad-range PCR/ESI-MS technology

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