Comparison of total antioxidant capacity of saliva in men based on daily cigarette consumption

Abstract

Objective: Antioxidants play an important role in inhibiting free oxygen and nitrogen radicals and preventing their formation. There are contradictory results regarding the relationship between cigarette smoking and total antioxidant capacity of saliva. In this study, the total antioxidant capacity of saliva has been compared in normal smokers, heavy smokers and non-smokers. Material and methods: In this cross-sectional study, 28 heavy male smokers (more than one pack of cigarettes per day), 28 normal male smokers (less than one pack of cigarettes per day), and 28 male non-smokers aged 25 to 40 years old entered the study. Unstimulated saliva was collected by Spitting method. The total antioxidant capacity of saliva was measured using Ferric Reducing Antioxidant Power Assay. Descriptive statistics, ANOVA, and Tukey tests were used to analyze the data. Results: The mean total antioxidant capacity of saliva in male non-smokers was 0.0598 ± 0.08 ?mol / L, in normal male smokers was 0.049 ± 0.04 ?mol / L, and in heavy male smokers was 0.0388 ± 0.035 ?mol / L, which did not show any significant difference between the groups (P > 0.05). Conclusion: The results of this study indicate that smoking does not have a significant effect on total antioxidant capacity of saliva in smokers. KEYWORDS Smokers; Saliva; Total antioxidant capacity.

A study on the correlation between smoking and non-enzymatic antioxidant factors of the saliva of healthy smokers and non-smokers

“…The subjects did not suffer from diseases such as diabetes, hypertension, thyroid disease or Sjögren's syndrome and without moderate to severe periodontal disease based on the Ramford index. It should be noted that subjects with poor oral health (With plaque index of 25% or higher) and history of medication and dietary supplement intake including hypertension medication, antiallergy medication, multivitamin supplements, and passive smokers were dropped out [15].…”

“…The participants of this study include 26 heavy smokers, 27 normal smokers, and 29 nonsmokers.Heavy smokers are defined as subjects who smoked more than 1 packet of cigarettes per day in the past 5 years.While normal smokers are comprised of subjects who smoked less than a packet per day during the aforementioned period [15]. Saliva samples were collected from 11:00 to 15:00 during the day.The subjects did not smoke nor ate or drank 90 minutes prior to saliva sampling, and they were seated during the sampling.…”