Comparison of toxicities from three metal oxide nanoparticles at environmental relevant concentrations in nematode Caenorhabditis elegans

Wu, Qiuli; Nouara, Abdelli; Li, Yiping; Zhang, Min; Wang, Wei; Tang, Meng; Ye, Boping; Ding, Jiandong; Wang, Dayong

doi:10.1016/j.chemosphere.2012.09.019

Cited by 152 publications

(72 citation statements)

References 54 publications

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“…Because growth of C. elegans is determined by a conservative genetic regulatory pathway, this endpoint test is a good parameter to evaluate toxic effects in C. elegans. 55 In the present study, we observed a significant reduction in body area between the control group and group VI (118.50×10…”

Section: Caenorhabditis Elegans As An Alternative In Vivo Modelsupporting

confidence: 53%

“…Only exposure to 50 µg/L of TiO 2 NPs and to ZnO NPs significantly reduced the body lengths of nematodes. 55 Another study demonstrated that the growth of nematodes was not affected by exposure to CeO 2 and TiO 2 NPs. 56 However, Cha et al showed that body length of nematodes exposed to fullerol NPs (diameter size: 4-40 nm) was significantly shorter than that of the nematodes in the control group.…”

Section: Caenorhabditis Elegans As An Alternative In Vivo Modelmentioning

confidence: 99%

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Caenorhabditis elegans as an alternative in vivo model to determine oral uptake, nanotoxicity, and efficacy of melatonin-loaded lipid-core nanocapsules on paraquat damage

Charão

Souto

Brucker

et al. 2015

IJN

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Caenorhabditis elegans is an alternative in vivo model that is being successfully used to assess the pharmacological and toxic effects of drugs. The exponential growth of nanotechnology requires the use of alternative in vivo models to assess the toxic effects of theses nanomaterials. The use of polymeric nanocapsules has shown promising results for drug delivery. Moreover, these formulations have not been used in cases of intoxication, such as in treatment of paraquat (PQ) poisoning. Thus, the use of drugs with properties improved by nanotechnology is a promising approach to overcome the toxic effects of PQ. This research aimed to evaluate the absorption of rhodamine B-labeled melatonin (Mel)-loaded lipid-core nanocapsules (LNC) by C. elegans , the application of this model in nanotoxicology, and the protection of Mel-LNC against PQ damage. The formulations were prepared by self-assembly and characterized by particle sizing, zeta potential, drug content, and encapsulation efficiency. The results demonstrated that the formulations had narrow size distributions. Rhodamine B-labeled Mel-LNC were orally absorbed and distributed in the worms. The toxicity assessment of LNC showed a lethal dose 50% near the highest dose tested, indicating low toxicity of the nanocapsules. Moreover, pretreatment with Mel-LNC significantly increased the survival rate, reduced the reactive oxygen species, and maintained the development in C. elegans exposed to PQ compared to those worms that were either untreated or pretreated with free Mel. These results demonstrated for the first time the uptake and distribution of Mel-LNC by a nematode, and indicate that while LNC is not toxic, Mel-LNC prevents the effects of PQ poisoning. Thus, C. elegans may be an interesting alternative model to test the nanocapsules toxicity and efficacy.

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Section: Caenorhabditis Elegans As An Alternative In Vivo Modelsupporting

confidence: 53%

Section: Caenorhabditis Elegans As An Alternative In Vivo Modelmentioning

confidence: 99%

Caenorhabditis elegans as an alternative in vivo model to determine oral uptake, nanotoxicity, and efficacy of melatonin-loaded lipid-core nanocapsules on paraquat damage

Charão

Souto

Brucker

et al. 2015

IJN

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“…The harmful effects of ZnONPs are driven by their physicochemical properties (dissolution and formation rate, the morphology and chemical composition, surface reactivity, particle number) and the resulting physical damage caused by the aggregation and agglomeration of nanoparticles (Bai et al, 2010;Jiang et al, 2009;Zhang et al, 2010. The bio-kinetic behaviour and in vivo toxicity of ZnONP exposure has, to date, been investigated in several non-mammalian systems including in vitro cell-based assays (Sharma et al, 2012a,b;Ahamed et al, 2011;Wu et al, 2010), bacteria (Li et al, 2011;Reddy et al, 2007), algae (Franklin et al, 2007), plants (Lin and Xing, 2007), crustaceans (Poynton et al, 2011), fish (Bai et al, 2010), earthworms (Hooper et al, 2011) and nematodes (Khare et al, 2011;Ma et al, 2009;Ma et al, 2011;Roh et al, 2009;Wu et al, 2013). The nematode Caenorhabditis elegans, a powerful model organism due to the availability of a completely sequenced genome (Hillier et al, 2005) and many molecular genetics tools has been used in ecotoxicological research to study the molecular to organismal level responses to ROS and heavy metal challenges (Roh et al, 2006;Hughes and Sturzenbaum 2007;Swain et al, 2004Swain et al, , 2010Zeitoun-Ghandour et al, 2010; The roles of the metalloproteins metallothionein (MT) and phytochelatin (PC) are Furthermore, ZnONP mediated toxicity may result from the release of free ionic zinc (George et al 2010;Li et al, 2012;Poynton et al, 2011;Wang et al, 2009), which induces cellular damage via the generation of free reactive oxygen species (ROS), which in turn can promote pro-inflammatory effects Mocchegiani et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Metalloproteins and phytochelatin synthase may confer protection against zinc oxide nanoparticle induced toxicity in Caenorhabditis elegans

Polak

Read

Jurkschat

et al. 2014

Comparative Biochemistry and Physiology Part C: Toxicology &

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Spurgeon, David J.; Sturzenbaum, Stephen R.. 2014. Metalloproteins and phytochelatin synthase may confer protection against zinc oxide nanoparticle induced toxicity in Caenorhabditis elegansContact CEH NORA team at noraceh@ceh.ac.ukThe NERC and CEH trademarks and logos ('the Trademarks') are registered trademarks of NERC in the UK and other countries, and may not be used without the prior written consent of the Trademark owner. suggesting that the nominal exposure to pure ZnONPs represents in vivo, at best, a mixture exposure of ionic zinc and nanoparticles. Nevertheless, the analyzes provided evidence that the metallothioneins (mtl-1 and mtl-2), the pytochelatin synthase (pcs-1) and an apoptotic marker (cep-1) were transcriptionally activated. In addition, the DCFH-DA assay provided in vitro evidence of the oxidative potential of ZnONPs in the metal exposure sensitive triple mutant.Raman spectroscopy highlighted that the biomolecular phenotype changes significantly in the metal sensitive knockout worm upon ZnONP exposure, suggesting that these metalloproteins are instrumental in the protection against cytotoxic damage. Finally, ZnONP exposure was shown to decrease growth and development, reproductive capacity and lifespan, effects which were amplified in the triple knockout. By combining diverse toxicological strategies, we identified that individuals (genotypes) housing mutations in key metalloproteins are more susceptible to ZnONP exposure, which underlines the importance of fully functional metalloproteins to minimize ZnONP induced toxicity.

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“…Low environ mentally relevant concentration of ZnO simulated using 0.05µg/ L ZnO NPs (Wu et al, 2013). Also, concentration of 500µg/L ZnO NPs was chosen as high non-lethal concentration based on a series of preliminary experiments (data not shown).…”

Section: Methodsmentioning

confidence: 99%

Untitled

Mansouri¹,

Johari²,

Azadi³

et al. 2018

Turk. J. Fish. Aquat. Sci.

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The aim of this study was comparing the toxic effects of zinc oxide nanoparticles (ZnO NPs) versus zinc ions (Zn 2+ ) at a high non-lethal (500µg/L) and a low environmental relevant (0.05µg/L) concentrations on gills of rainbow trout (Oncorhynchus mykiss) following 14 days of waterborne exposure. Structural alterations, histopathological anomalies, and zinc bioaccumulation were investigated in the gills using field emission scanning electron microscopy (FESEM ), hematoxylin and eosin staining (H&E), and graphite furnace atomic absorption spectrophotometry (GFAAS) respectively. Some damages such as shortening and fusion of secondary lamellae, surface epithelium hypertrophy, and hyperplasia of the primary lamellae were observed in the gill tissue. Histopathological alterations of gills were minimum in bot h none exposed (control) fish and fish exposed to 0.05µg/L Zn 2+ . The severity of gill damages were higher in fish exposed to 500µg/L ZnO NPs compared to 500µg/L Zn 2+ and 0.05µg/L ZnO NPs. The Zn accumulation in the gills was concentration-dependent such that bioaccumulation order was as 500µg/L Zn 2+ > 500µg/L ZnO NPs ≈ 0.05µg/L Zn 2+ > 0.05µg/L ZnO NPs> control. In summary, the results of present study showed that although the accumulation capability of Zn 2+ was higher than ZnO NPs, but NPs cause more structural damages to gills compare to ions.

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Comparison of toxicities from three metal oxide nanoparticles at environmental relevant concentrations in nematode Caenorhabditis elegans

Cited by 152 publications

References 54 publications

Caenorhabditis elegans as an alternative in vivo model to determine oral uptake, nanotoxicity, and efficacy of melatonin-loaded lipid-core nanocapsules on paraquat damage

Caenorhabditis elegans as an alternative in vivo model to determine oral uptake, nanotoxicity, and efficacy of melatonin-loaded lipid-core nanocapsules on paraquat damage

Metalloproteins and phytochelatin synthase may confer protection against zinc oxide nanoparticle induced toxicity in Caenorhabditis elegans

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