Comparison of traditional statistical quality control using commercially available control materials and two patient‐based quality control procedures for the <scp>ADVIA</scp> 120 Hematology System

Vanyo, Lourdes C.; Freeman, Kathleen; Meléndez-Lazo, Antonio; Cuenca, Rafaela; Pastor, Josep

doi:10.1111/vcp.12645

Cited by 7 publications

(12 citation statements)

References 16 publications

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“…19,20 These findings could explain the initially sta- In one report of duplicate analysis of canine platelet counts using the first-generation ADVIA 120 over 25 days, the overall CV was 12.23%. 35 The CVs calculated in our study were similar, except for Citrate ADP and AGGFix ADP. The higher CV for Citrate ADP platelet counts likely reflects both less precision of platelet counting in citrate compared with EDTA, 28 and possibly rapid changes in platelet aggregation between duplicate runs, as during optimizing studies, very rapid platelet disaggregation was noted (data not shown).…”

Section: Discussionsupporting

confidence: 74%

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Extended sample storage for platelet function testing in healthy dogs

Dickinson

Abrams‐Ogg

Blois

et al. 2023

Veterinary Clinical Pathol

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Background: Platelet function testing is important for monitoring the effects of antiplatelet therapy but is not readily used due to time constraints for testing and the need for specialized equipment.Objectives: This study evaluated the effects of various storage methods on selected platelet function tests to determine if delayed platelet function testing is feasible in canine blood samples. Our hypotheses were that platelet function would not decline during storage and, thus, no differences in test results would be found over time.Methods: Thirteen healthy dogs were studied. Citrated blood samples were tested with a Platelet Function Analyzer-200 (PFA), which mimics high-shear conditions, using P2Y and CADP cartridges, after being held at room temperature for 2 h and refrigerated for 24 and 48 h. Plateletworks (PW), which measures aggregation based on platelet counting, was performed on an optical hematology analyzer using 10-min-old native samples, citrated samples held at room temperature for 3-4 h and refrigerated for 24 and 48 h, and samples stored in the preservative solution, AGGFix, up to 7 days.Results: PFA closure times increased with storage, especially with the P2Y cartridge.Median aggregation with fresh PW was 94%, and this was maintained at all time points (range of median values 88%-94%). Most samples showed decreased, yet still robust (>70%), aggregation with longer storage. Spontaneous aggregation in citrate was noted in most dogs. AGGFix stabilized platelet aggregates to allow for delayed testing.Conclusions: Delayed platelet function testing is feasible, but ranges of expected values may differ from tests using fresh samples.

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Section: Discussionsupporting

confidence: 74%

“…Equivalent data for dogs are not available. In one report of duplicate analysis of canine platelet counts using the first‐generation ADVIA 120 over 25 days, the overall CV was 12.23% 35 . The CVs calculated in our study were similar, except for Citrate ADP and AGGFix ADP.…”

Section: Discussionsupporting

confidence: 72%

Extended sample storage for platelet function testing in healthy dogs

Dickinson

Abrams‐Ogg

Blois

et al. 2023

Veterinary Clinical Pathol

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“…This differed from prior recommendations to collect 20 data points 9 and prior studies that were able to validate limits determined using 20 data points. [10][11][12] Following the RPT-QC limit generation, the validation process required calculation of limits using 30-40 data points for more than half of measurands/time interval evaluations. We found that by only using 20 results, the data were either not included within the control limits or did not adequately fill the range, but when an additional 10-20 data points were used, then we saw a better distribution across the QC data range.…”

Section: Discussionmentioning

confidence: 99%

“…9 The difference between original and repeated analyses can then be calculated and plotted similarly to other QC procedures. 7 There have been some positive reports in the literature [10][11][12] that indicate a good potential for application in veterinary laboratories.…”

Section: Introductionmentioning

confidence: 99%

Repeat patient testing‐quality control with canine samples shows promise as an alternative to commercial quality control material for a network of four Sysmex XT‐2000iV hematology analyzers

Daly

Graham

Freeman³

2023

Veterinary Clinical Pathol

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BackgroundRepeat patient testing‐quality control (RPT‐QC) uses retained patient samples as an alternative to commercial quality control material (QCM). We elected to calculate and validate RPT‐QC limits for red blood cell count (RBC), hemoglobin (HBG), hematocrit (HCT), and white blood cell count (WBC).Objectives(1) To validate RPT‐QC across a network of four harmonized Sysmex XT‐2000iV hematology analyzers and determine the total error that can be controlled with RPT‐QC. (2) To generate quality control (QC) limits using the standard deviation (SD) of the duplicate measurement differences and determine a suitable simple QC rule with a probability of error detection >0.85 and probability of false rejection <0.05. (3) Monitor RPT‐QC using sigma metrics as a performance indicator and (4) to challenge RPT‐QC to ensure acceptable sensitivity.MethodsFresh adult canine EDTA samples with results within reference intervals were selected and run again on days 2, 3, and 4. QC limits were generated from the SD of the duplicate measurement differences. The QC limits were challenged using interventions designed to promote unstable system performance. The total error detectable by RPT‐QC was determined using EZRULES 3 software.ResultsIn all, 20‐40 data points were needed for RPT‐QC calculations and validated using 20 additional data points. The calculated limits differed among the network of analyzers. The total error that could be controlled was the same or better than that of the manufacturer's commercially available quality control material using the same analyzer for all measurands except hematocrit, which required a higher total error goal than that proposed by ASVCP guidelines to achieve an acceptable probability of error detection. The challenges designed to mimic unstable system performance were successfully detected as out‐of‐control QC.ConclusionsThe challenges for RPT‐QC resulted in acceptable detection of potential unstable system performance. This initial study demonstrates that RPT‐QC limits differ among the network of Sysmex XT‐2000iV analyzers, indicating a requirement to customize for the individual analyzer and laboratory conditions. RPT‐QC could achieve ASVCP total allowable error goals for RBC, HGB, and WBC, but not for HCT. Sigma metrics were consistently >5.5 for RBC, HGB, and WBC, but not for HCT.

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“…Several methods using patient samples have been described and found to be both practical and successful at evaluating the function of veterinary hematology analyzers. [16][17][18] These strategies each have their own benefits and drawbacks.…”

Section: Opp Ortunitie S For Improving the Future Of Qcmentioning

confidence: 99%

Improving quality control for in‐clinic hematology analyzers: Common myths and opportunities

Michael

Nabity

Couto³

et al. 2022

Veterinary Clinical Pathol

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Comparison of traditional statistical quality control using commercially available control materials and two patient‐based quality control procedures for the ADVIA 120 Hematology System

Abstract: Patient-based procedures are suitable for veterinary laboratories. The RPT approach may benefit laboratories with limited budgets and low hematology caseloads. The AoN procedure may benefit laboratories with higher hematology caseloads.

Cited by 7 publications

References 16 publications

Extended sample storage for platelet function testing in healthy dogs

Extended sample storage for platelet function testing in healthy dogs

Repeat patient testing‐quality control with canine samples shows promise as an alternative to commercial quality control material for a network of four Sysmex XT‐2000iV hematology analyzers

Improving quality control for in‐clinic hematology analyzers: Common myths and opportunities

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