Comparison of Transcriptome Profiles of the Fungus Botrytis cinerea and Insect Pest Bradysia odoriphaga in Response to Benzothiazole

Cui, Kaidi; Zhao, Yunhe; He, Lifei; Ding, Jinfeng; Li, Beixing; Mu, Wei; Feng, Liu

doi:10.3389/fmicb.2020.01043

Cited by 9 publications

(9 citation statements)

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“…In this regard, the strong upregulation of several melanin biosynthesis and laccase genes was observed in our RNA-seq analysis, a result confirmed by the browning of the treated mycelium observed two days after peptide treatment. Melanin in fungi is considered to play a protective role ( Cordero and Casadevall, 2017 ; Cui et al, 2020 ) and strengthen the cell wall by preventing the penetration of the peptide into the fungal cells. Fungal laccases are also reported to perform several functions such as pigmentation (dihydroxynaphthalene melanin, produced against environmental stress) and detoxification ( Lu et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and Ultrastructural Analyses of Pyricularia Oryzae Treated With Fungicidal Peptaibol Analogs of Trichoderma Trichogin

et al. 2021

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Eco-friendly analogs of Trichogin GA IV, a short peptaibol produced by Trichoderma longibrachiatum, were assayed against Pyricularia oryzae, the causal agent of rice blast disease. In vitro and in vivo screenings allowed us to identify six peptides able to reduce by about 70% rice blast symptoms. One of the most active peptides was selected for further studies. Microscopy analyses highlighted that the treated fungal spores could not germinate and the fluorescein-labeled peptide localized on the spore cell wall and in the agglutinated cytoplasm. Transcriptomic analysis was carried out on P. oryzae mycelium 3 h after the peptide treatment. We identified 1,410 differentially expressed genes, two-thirds of which upregulated. Among these, we found genes involved in oxidative stress response, detoxification, autophagic cell death, cell wall biogenesis, degradation and remodeling, melanin and fatty acid biosynthesis, and ion efflux transporters. Molecular data suggest that the trichogin analogs cause cell wall and membrane damages and induce autophagic cell death. Ultrastructure observations on treated conidia and hyphae confirmed the molecular data. In conclusion, these selected peptides seem to be promising alternative molecules for developing effective bio-pesticides able to control rice blast disease.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic and Ultrastructural Analyses of Pyricularia Oryzae Treated With Fungicidal Peptaibol Analogs of Trichoderma Trichogin

et al. 2021

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“…Among the ten obtained pathways, OF caused significant change in five metabolic pathways, including pyrimidine metabolism, galactose metabolism, pentose and glucuronate interconversions, streptomycin biosynthesis, and C 5 -branched dibasic acid metabolism (Figure 8). Interestingly, the pentose and glucuronate interconversions pathway was shared by both Botrytis cinerea and Bradysia odoriphaga [63]. Furthermore, Streptomycin is one kind of antibiotics, which could be acted as both an offensive and defensive weapon that facilitates invasion into occupied habitats and also protects against invasion by competitors [64].…”

Section: The Effect Of Compound/bio-organic Fertilizer On the Metabolic Pathwaysmentioning

confidence: 99%

Effect of Two Kinds of Fertilizers on Growth and Rhizosphere Soil Properties of Bayberry with Decline Disease

Ren

Wang

et al. 2021

Plants

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Decline disease causes severe damage to bayberry. However, the cause of this disease remains unclear. Interestingly, our previous studies found that the disease severity is related with the level of soil fertilizer. This study aims to explore the effect and mechanism of compound fertilizer (CF) and bio-organic fertilizer (OF) in this disease by investigating the vegetative growth, fruit characters, soil property, rhizosphere microflora and metabolites. Results indicated that compared with the disease control, CF and OF exhibited differential effect in plant healthy and soil quality, together with the increase in relative abundance of Burkholderia and Mortierella, and the reduction in that of Rhizomicrobium and Acidibacter, Trichoderma, and Cladophialophora reduced. The relative abundance of Geminibasidium were increased by CF (251.79%) but reduced by OF (13.99%). In general, the composition of bacterial and fungal communities in rhizosphere soil was affected significantly at genus level by exchangeable calcium, available phosphorus, and exchangeable magnesium, while the former two variables had a greater influence in bacterial communities than fungal communities. Analysis of GC-MS metabonomics indicated that compared to the disease control, CF and OF significantly changed the contents of 31 and 45 metabolites, respectively, while both fertilizers changed C5-branched dibasic acid, galactose, and pyrimidine metabolic pathway. Furthermore, a significant correlation was observed at the phylum, order and genus levels between microbial groups and secondary metabolites of bayberry rhizosphere soil. In summary, the results provide a new way for rejuvenation of this diseased bayberry trees.

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“…Transcriptome analysis of B. cinerea treated with benzothiazole was performed to clarify its antifungal mechanism. 59 iTRAQ-based quantitative proteomics identified 14 benzothiazole-responsive proteins in Bradysia odoriphaga. 60 Omics techniques have greatly promoted the understanding of VOCs molecular mechanism, but the resulting high-throughput data hinder subsequent functional analysis and phenotypic determination.…”

Section: ■ Discussionmentioning

confidence: 99%

“…Transcriptome analysis of B. cinerea treated with benzothiazole was performed to clarify its antifungal mechanism . iTRAQ-based quantitative proteomics identified 14 benzothiazole-responsive proteins in Bradysia odoriphaga .…”

Section: Discussionmentioning

confidence: 99%

Green Leaf Volatile Trans-2-Hexenal Inhibits the Growth of Fusarium graminearum by Inducing Membrane Damage, ROS Accumulation, and Cell Dysfunction

Wang

Zhu

et al. 2022

J. Agric. Food Chem.

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Fusarium graminearum, the main agent of Fusarium head blight (FHB), can cause serious yield loss and secrete mycotoxins to contaminate grain. Here, the biological activity of trans-2-hexenal (T2H) against F. graminearum was determined and its mode of action (MOA) was investigated. Furthermore, surface plasmon resonance with liquid chromatography–tandem mass spectrometry (SPR-LC–MS/MS), bioinformatic analysis, and gene knockout technique were combined to identify the binding proteins of T2H in F. graminearum cells. T2H exhibited satisfactory inhibitory activity against F. graminearum in vitro. Good lipophilicity greatly enhanced the affinity of T2H to F. graminearum mycelia and further caused membrane damage. The FgTRR (thioredoxin reductase) gene negatively regulates the sensitivity of F. graminearum to T2H by reducing the generation of reactive oxygen species (ROS) induced by T2H. Two mutant strains with FgSLX1 (structure-specific endonuclease subunit) and FgCOPB (coatomer subunit β) genes knockout showed decreased sensitivity to T2H, suggesting that these two genes may be involved in the antimicrobial activity of T2H. Taken together, T2H can inhibit F. graminearum growth by multiple MOAs and can be used as a biofumigant to control the occurrence of FHB in the field.

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Comparison of Transcriptome Profiles of the Fungus Botrytis cinerea and Insect Pest Bradysia odoriphaga in Response to Benzothiazole

Cited by 9 publications

References 83 publications

Transcriptomic and Ultrastructural Analyses of Pyricularia Oryzae Treated With Fungicidal Peptaibol Analogs of Trichoderma Trichogin

Transcriptomic and Ultrastructural Analyses of Pyricularia Oryzae Treated With Fungicidal Peptaibol Analogs of Trichoderma Trichogin

Effect of Two Kinds of Fertilizers on Growth and Rhizosphere Soil Properties of Bayberry with Decline Disease

Green Leaf Volatile Trans-2-Hexenal Inhibits the Growth of Fusarium graminearum by Inducing Membrane Damage, ROS Accumulation, and Cell Dysfunction

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