Comparison of transgenic<i>Bt</i>rice and its non‐<i>Bt</i>counterpart in adaptability to nitrogen deficiency

Jiang, Yang; Ling, Lin; Zhan, Mei; Li, Chengfang; Cao, Cougui

doi:10.1002/jpln.201700470

Cited by 5 publications

(2 citation statements)

References 52 publications

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“…It also was found that the high Cry1Ca accumulation in plants had strong unintended effects 13 . Compared with the receptor variety, the Bt rice Huahui‐1 showed reduced adaptability to nitrogen deficiency, 45 drought stress 46 and saline‐alkaline stress 47 . Further studies showed that the unintended effects of Huahui‐1 may result from cellular localization 17 and protein interactions of exogenous Bt protein 16 .…”

Section: Discussionmentioning

confidence: 99%

Cell wall‐localized Bt protein endows rice high resistance to Lepidoptera pests

Li,

Deng,

Weng

et al. 2023

Pest Management Science

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BACKGROUNDThe commercialized Bt (Bacillus thuringiensis) crops accumulate Bt protein within cells, but the intracellular interactions of foreign protein with endogenous protein inevitably result in large or small unintended effects. In this study, the Bt gene Cry1Ca was linked with the sequences of extracellular secretion signal peptide and carbohydrate binding module 11 to constitute a fusion gene SP‐Cry1Ca‐CBM11, and the fusion gene driven by constitutive promoters was used for secreting and anchoring onto the cell wall to minimize unintended effects.RESULTSThe transient expression in tobacco leaves demonstrated that the fusion protein was anchored on cell walls. The Cry1Ca contents of five homozygous rice transformants of single‐copy insertion were different and descended in the order leaf > root > stem. The maximum content of Cry1Ca was 17.55 μg g−1 in leaves of transformant 21H037. The bioassay results revealed that the transformants exhibited high resistance to lepidopteran pests. The corrected mortality of pink stem borer (Sesamia inferens) and striped stem borer (Chilo suppressalis) ranged from 96.33% to 100%, and from 83.32% to 100%, respectively, and the corrected mortality of rice leaf roller (Cnaphalocrocis medinalis) was 92.53%. Besides, the agronomic traits of the five transformants were normal and similar to that of the recipient, and the transformants were highly resistant to glyphosate at the germination and seedling stages.CONCLUSIONThe fusion Bt protein was accumulated on cell walls and endowed the rice with high resistance to lepidopteran pests without unintended effects in agronomic traits. © 2023 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 99%

Cell wall‐localized Bt protein endows rice high resistance to Lepidoptera pests

Li,

Deng,

Weng

et al. 2023

Pest Management Science

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“…We previously reported that an array of agronomic traits, including tiller number, biomass, and grain weight per plant, were significantly lower in insect-resistant transgenic cry1Ab/c rice Huahui-1 (HH1) than those in the parental line Minghui-63 (MH63) under saline-alkaline stress ( Fu et al, 2018 ). Moreover, HH1 showed reduced adaptability to nitrogen deficiency and drought than that its parental line MH63, which may be related to high Bt protein expression in the transgenic line ( Jiang et al, 2018a , b ). However, the underlying molecular mechanisms remain unknown.…”

Section: Introductionmentioning

confidence: 97%

Cellular Localization of Exogenous Cry1Ab/c and its Interaction with Plasma Membrane Ca2+-ATPase in Transgenic Rice

Shi

Liu

et al. 2021

Front. Bioeng. Biotechnol.

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The cellular localization of exogenous proteins expressed in transgenic crops not only determines their stability, but also their effects on crop growth and development, including under stressful conditions; however, the underlying molecular mechanisms remain unknown. Here, we determined the cellular distribution of exogenously expressed Cry1Ab/c protein in insect-resistant transgenic rice Huahui-1 (HH1) cells through subcellular localization, immunohistochemistry, immunofluorescence, and western blot analyses. Interaction between the Cry1Ab/c protein and the preliminarily screened endogenous plasma membrane protein Ca2+-ATPase was investigated through yeast two-hybrid, bimolecular fluorescence complementation (BIFC), and co-immunoprecipitation analyses. The potential interaction mechanism was analyzed by comparing the cellular localization and interaction sites between Cry1Ab/c and Ca2+-ATPase. Phenotypic indices and Ca2+-ATPase activity, which may be regulated by the Cry1Ab/c–Ca2+-ATPase interaction, were determined in transgenic HH1 and the parental line Minghui-63 under stress-free and salt-stress conditions. The results showed that Cry1Ab/c was not only distributed in the cytoplasm and nucleus but was also distributed on the plasma membrane, where it interacted with plasma membrane Ca2+-ATPase. This interaction partially retain plasma membrane protein Ca2+-ATPase in the nucleus by a BIFC experiment and thus may affect Ca2+-ATPase activity on the membrane by altering the cellular location of the protein. Consistently, our results confirmed that the presence of Cry1Ab/c in the transgenic HH1 resulted in a reduction in Ca2+-ATPase activity as well as causing detrimental effects on plant phenotype, including significantly reduced plant height and biomass, compared to parental MH63; and that these detrimental effects were more pronounced under salt stress conditions, impacting the salt resistance of the transgenic plants. We suggest that the Cry1Ab/c–Ca2+-ATPase interaction may explain the plasma membrane localization of Cry1Ab/c, which lacks a signal peptide and a transmembrane domain, and the adverse effects of Cry1Ab/c expression on the growth and development of transgenic HH1 plants under salt stress. This information may clarify the molecular mechanisms of these unintended effects and demonstrate the feasibility of evaluating the success and performance of genetic modification of commercially vital crops.

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Effects of Nitrogen Fertilizer on the Bt Protein Content in Transgenic Rice and Nitrogen Metabolism Mechanism

Chen

Jiang

2021

J Plant Growth Regul

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Comparison of transgenicBtrice and its non‐Btcounterpart in adaptability to nitrogen deficiency

Cited by 5 publications

References 52 publications

Cell wall‐localized Bt protein endows rice high resistance to Lepidoptera pests

Cell wall‐localized Bt protein endows rice high resistance to Lepidoptera pests

Cellular Localization of Exogenous Cry1Ab/c and its Interaction with Plasma Membrane Ca2+-ATPase in Transgenic Rice

Effects of Nitrogen Fertilizer on the Bt Protein Content in Transgenic Rice and Nitrogen Metabolism Mechanism

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