Comparison of tricaine methanesulphonate (MS222) and clove oil anaesthesia effects on the physiology of juvenile chinook salmonOncorhynchus tshawytscha(Walbaum)

Abstract

This study investigated the feasibility of using clove oil as an alternative to tricaine methanesulphonate as a ®sh anaesthetic, particularly in ®sh stress research. The physiological stress responses of juvenile chinook salmon Oncorhynchus tshawytscha (Walbaum) anaesthetized with either tricaine (50 mg L ±1 ) or clove oil (20 p.p.m.) were compared using unanaesthetized ®sh as controls. Haematocrit, serum cortisol and serum glucose concentrations, serum lysozyme activity and differential leucocyte counts were measured from blood samples collected before, during and upon recovery from anaesthesia and at speci®ed intervals up to 72 h after recovery. Differences between the two anaesthetic groups were not signi®cant for most of the physiological traits measured. Serum lysozyme activity of control ®sh, however, was signi®cantly suppressed relative to the treated ®sh for 72 h after stress. Clove oil may be a safe and cost-effective alternative to tricaine without signi®cantly affecting study results. Furthermore, clove oil may be more practical for ®eld-based research, because a withdrawal period is unnecessary, and clove oil does not pose an environmental hazard.

Effects of clove oil on the stress response of matrinxã (Brycon cephalus) subjected to transport

“…Clove oil is approved for use in aquaculture facilities in Australia, New Zealand, and other Oceania countries with no withdrawal period for human consumption and release of the fishes in the environment (Kildea et al, 2004). However, in North America the FDA does not approve the use of clove oil for aquaculture purposes (Sladky et al, 2001) Researchers have investigated the effects of clove oil in fishes (Soto & Burhanuddin, 1995;Keene et al, 1998;Cho & Heath, 2000;Sladky et al, 2001;Tort et al, 2002;Woody et al, 2002;Small, 2004). A study demonstrating the efficacy of clove oil in matrinxã (Inoue et al, 2003), but this work only determined the induction and recovery times to anesthesia.…”

The effects of four anaesthetics on haematological and blood biochemical profiles in vimba bream, Vimba vimba

“…A variety of anaesthetics with differing properties have been used in aquaculture (Cho and Heath 2000;Kazun and Siwicki 2001;Velisek and Svobodova 2004a,b;Velisek et al 2005aVelisek et al ,b, 2006Velisek et al , 2007Velisek et al , 2009Velisek et al , 2011. Chemicals used in aquaculture are subject to strict control, particularly with regard to safety and efficacy (Taylor and Roberts 1999).…”

Anestesia do pirarucu por aspersão direta nas brânquias do eugenol em solução aquosa

“…Esse é o principal componente do óleo-de-cravo e de vários outros óleos essenciais extraídos de plantas amazônicas, apresentando poucos riscos ao ambiente e aos animais (CHO & HEATH, 2000;INOUE et al, 2003;IVERSEN et al, 2003;TORT et al, 2002). A eliminação do eugenol do tecido comestível -NOTA - em peixes pré-expostos é em torno de 12h (KILDEA et al, 2004).…”