Comparison of two beta-lactamase-producing strains of Streptococcus faecalis

Murray, Barbara E.; Church, D.; Wanger, Audrey; Zscheck, Karen K.; Levison, Matthew E.; Ingerman, Mark J.; Abrutyn, Elías; Mederski-Samoraj, Barbara

doi:10.1128/aac.30.6.861

Cited by 100 publications

(80 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3A and Table 1). The earliest isolate of this clone, HH-22, the first known Bla ϩ isolate of E. faecalis, is a multidrug-resistant urine isolate recovered in Texas in 1981 (18); the blaZ gene of this strain is located on a plasmid that also harbors a gene for high-level gentamicin resistance (HL-Gm r ) (16). Other members of this clone (which represents the majority of known Bla ϩ isolates) were subsequently found in five states of North America (20), including a large, prolonged outbreak in a Virginia hospital (33,40) and a hospital outbreak that included five bloodstream infections in North Carolina (13).…”

Section: Vol 187 2005 Evolution Of An E Faecalis Clonal Complex Anmentioning

confidence: 99%

Molecular Characterization of a Widespread, Pathogenic, and Antibiotic Resistance-Receptive Enterococcus faecalis Lineage and Dissemination of Its Putative Pathogenicity Island

Nallapareddy¹,

Huang²,

Weinstock

et al. 2005

J Bacteriol

View full text Add to dashboard Cite

Enterococcus faecalis, a common cause of endocarditis and known for its capacity to transfer antibiotic resistance to other pathogens, has recently emerged as an important, multidrug-resistant nosocomial pathogen. However, knowledge of its lineages and the potential of particular clones of this species to disseminate and cause disease is limited. Using a nine-gene multilocus sequence typing (MLST) scheme, we identified an evolving and widespread clonal complex of E. faecalis that has caused outbreaks and life-threatening infections. Moreover, this unusual clonal complex was found to contain isolates of unexpected relatedness, including the first known U.S. vancomycin-resistant enterococcus (E. faecalis strain V583), the first known penicillinaseproducing (Bla ؉ ) E. faecalis isolate, and the previously described widespread clone of penicillinase producers, a trait found in <0.1% of E. faecalis isolates. All members of this clonal cluster (designated as BVE for Bla ؉ Van r endocarditis) were found to contain a previously described putative pathogenicity island (PAI). Further analysis of this PAI demonstrated its dissemination worldwide, albeit with considerable variability, confirmed its association with clinical isolates, and found a common insertion site in different clonal lineages. PAI deletions, MLST, and the uncommon resistances were used to predict the evolution of the BVE clonal cluster. The finding of a virulent and highly successful clonal complex of E. faecalis with different members resistant to the primary therapies of choice, ampicillin and vancomycin, has important implications for the evolution of virulence and successful lineages and for public health monitoring and control.

show abstract

Section: Vol 187 2005 Evolution Of An E Faecalis Clonal Complex Anmentioning

confidence: 99%

Molecular Characterization of a Widespread, Pathogenic, and Antibiotic Resistance-Receptive Enterococcus faecalis Lineage and Dissemination of Its Putative Pathogenicity Island

Nallapareddy¹,

Huang²,

Weinstock

et al. 2005

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…Although the gene responsible for enterococcal ,-lactamase production is homologous with that found in Staphylococcus aureus, its phenotypic expression differs in that enterococcal P-lactamase is produced constitutively, is not excreted into the medium, and has only a minimal effect on the penicillin MIC at standard inoculum sizes (16). Genetic studies on five strains previously described have revealed the presence of the 1-lactamase genes on conjugative plasmids in close association with genes encoding enzymes conferring highlevel resistance to gentamicin (16,19,20).…”

mentioning

confidence: 99%

Chromosomally mediated beta-lactamase production and gentamicin resistance in Enterococcus faecalis

Rice

Eliopoulos

Wennersten

et al. 1991

Antimicrob Agents Chemother

View full text Add to dashboard Cite

We have analyzed four distinct strains of multiply resistant, beta-lactamase-producing enterococci isolated during an outbreak of colonization with these strains on an infant-toddler surgical ward at The Children's Hospital in Boston, Mass. All four strains were resistant to erythromycin, penicillin, and tetracycline and to high levels of gentamicin and streptomycin. One strain was also resistant to chloramphenicol. Plasmid profiles revealed four different plasmid patterns, with the number of identified plasmids ranging from zero to three. The gene coding for beta-lactamase production could be transferred at low frequency (less than 10(-8)) to an enterococcal recipient from one strain in conjunction with all of the other resistance determinants. Probes derived from the staphylococcal beta-lactamase gene and gentamicin resistance gene failed to hybridize with any of the detectable plasmids, but both genes were present on restriction fragments of genomic DNA in all strains. Our results indicate that the beta-lactamase genes and gentamicin resistance genes in these strains are integrated into the bacterial chromosome. The cotransmissibility of the resistance determinants raises the possibility of their incorporation into a multiresistance transposable genetic element.

show abstract

“…This strain also carried high-level resistance to all aminoglycosides. It is not surprising to find both penicillinase production and gentamicin resistance in the same bacterium, since both can be encoded on transferrable plasmids (42,43). Although penicillinase-producing enterococci appear to be rare, a few other strains have been isolated (24,45,46).…”

Section: Beta-lactam Resistancementioning

confidence: 99%

“…In recent years, however, increasing numbers of enterococcal isolates have acquired high-level resistance to the aminoglycosides (2,4,19,20,23,65). Enterococci for which MICs are above clinically achievable levels of the penicillins and vancomycin in serum have also been reported (19,31,42,43,57,59). Serious infections with these multiple-drug-resistant strains may not be curable with the traditional therapy.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial resistance among enterococci

Herman

Gerding

1991

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Comparison of two beta-lactamase-producing strains of Streptococcus faecalis

Abstract: A second strain of enterococcus (PA) producing beta-lactamase (Bla+ phenotype) was

Cited by 100 publications

References 17 publications

Molecular Characterization of a Widespread, Pathogenic, and Antibiotic Resistance-Receptive Enterococcus faecalis Lineage and Dissemination of Its Putative Pathogenicity Island

Molecular Characterization of a Widespread, Pathogenic, and Antibiotic Resistance-Receptive Enterococcus faecalis Lineage and Dissemination of Its Putative Pathogenicity Island

Chromosomally mediated beta-lactamase production and gentamicin resistance in Enterococcus faecalis

Antimicrobial resistance among enterococci

Contact Info

Product

Resources

About