Comparison of two commercial enzyme-linked immunosorbent assays for detection of <i>Influenza A virus</i> antibodies

Lebarbenchon, Camille; Brown, Justin D.; Luttrell, M. Page; Stallknecht, David E.

doi:10.1177/1040638711416626

Cited by 27 publications

(20 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our results are consistent with previous studies and suggest that surveillance for virus shedding alone may provide incomplete information on the transmission potential relative to surveys which also include detection of antibodies [25], [63]. Indirect techniques such as ELISA have potentially valuable applications for AIVs monitoring in bird species and should be considered when deciphering patterns of exposure, differential infection, and rates of AIVs transmission [25], [64].…”

Section: Discussionsupporting

confidence: 85%

Surveillance of Influenza Viruses in Waterfowl Used As Decoys in Andalusia, Spain

et al. 2014

View full text Add to dashboard Cite

A longitudinal study was carried out to determine the seroprevalence of avian influenza viruses (AIVs) in waterfowl used as decoys in Andalusia, southern Spain. A total of 2319 aquatic birds from 193 flocks were analyzed before and after the hunting season 2011–2012. In the first sampling, 403 out of 2319 (18.0%, CI95%: 15.8–19.0) decoys showed antibodies against AIVs by ELISA. The AI seroprevalence was significantly higher in geese (21.0%) than in ducks (11.7%) (P<0.001). Besides, the spatial distribution of AIVs was not homogeneous as significant differences among regions were observed. The prevalence of antibodies against AIVs subtypes H5 and H7 were 1.1% and 0.3%, respectively, using hemagglutination inhibition test (HI). The overall and H5 seroprevalences slightly increased after the hunting period (to 19.2% and 1.4%, respectively), while the H7 seroprevalence remained at the same level (0.3%). The proportion of flocks infected by AIVs was 65.3%, while 11.2% and 4.9% of flocks were positive for H5 and H7, respectively. Viral shedding was not detected in any of the 47 samples positive by both ELISA and HI, tested by RRT-PCR. The individual incidence after the hunting season was 3.4%. The fact that 57 animals seroconverted, 15 of which were confirmed by HI (12 H5 and 3 H7), was indication of contact with AIVs during the hunting period. The results indicate that waterfowl used as decoys are frequently exposed to AIVs and may be potentially useful as sentinels for AIVs monitoring. The seroprevalence detected and the seropositivity against AIVs H5 and H7, suggest that decoys can act as reservoirs of AIVs, which may be of animal and public health concern.

show abstract

Section: Discussionsupporting

confidence: 85%

Surveillance of Influenza Viruses in Waterfowl Used As Decoys in Andalusia, Spain

et al. 2014

View full text Add to dashboard Cite

show abstract

“…For LP IA viruses, serum samples were obtained from 43 one‐month‐old Mallards experimentally infected with eight different virus subtypes (Table ) as well as from eight sham‐inoculated birds . Blood samples were collected at the end of the experiments (14 or 21 days post‐infection) and sera stored at −20°C until tested.…”

Section: Effect Of the Incubation Period And Sample Dilution Factor Omentioning

confidence: 99%

Evaluation of a commercial enzyme‐linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl

Lebarbenchon

Pantin‐Jackwood

Kistler

et al. 2012

Influenza Resp Viruses

Self Cite

View full text Add to dashboard Cite

The ID Screen Influenza H5 Antibody Competition enzyme‐linked immunosorbent assay was tested for the detection of antibodies to the H5 subtype of influenza A (IA) virus in waterfowl. Assays were conducted with sera obtained from Mallards (Anas platyrhynchos) and Pekin Ducks (Anas platyrhynchos domestica), experimentally infected with eight low pathogenic (LP) and nine highly pathogenic (HP) H5N1 IA viral strains. Three incubation periods (1, 4 and 18 hours) and two dilutions (1:2 and 1:5) were tested. All serum samples from LP H5‐infected birds tested positive; however, improved detection rates were observed for viruses belonging to the HP H5N1 clade 2.2.1 as compared with those belonging to clade 2.1.3.

show abstract

“…However, serological assays have been developed recently that have a high sensitivity at detecting antibodies to IAVs, thus these assays can be used to improve surveillance approaches (Brown et al, 2009; Lebarbenchon et al, 2012). The duration of detectable antibodies can be >1 year in naturally infected ducks (Tolf et al, 2013), and with repeated infections, they may persist for the life of the bird.…”

Section: Introductionmentioning

confidence: 99%

Subtype-specific influenza A virus antibodies in Canada geese (Branta canadensis)

Kistler

Stallknecht

DeLiberto

et al. 2015

Veterinary Microbiology

Self Cite

View full text Add to dashboard Cite

Historically, surveillance for influenza A viruses (IAVs) in wild birds has relied on viral detection assays. This was largely due to poor performance of serological assays in wild birds; however, recently developed commercial serological assays have improved the ability to detect IAV antibodies in wild birds. Serological surveillance for IAV antibodies in Canada geese (Branta canadensis) has shown that, despite a low prevalence of virus isolations, Canada geese are frequently exposed to IAVs and that exposure increases with latitude, which follows virus isolation prevalence patterns observed in dabbling ducks. The objectives of this study were to further evaluate IAV antibodies in Canada geese using a subtype-specific serological assay to determine if Canada geese are exposed to subtypes that commonly circulate in dabbling ducks. We collected serum samples from Canada geese in Minnesota, New Jersey, Pennsylvania, and Wisconsin and tested for antibodies to IAVs using a blocking ELISA. Positive samples were further tested by hemagglutination inhibition for 10 hemagglutinin IAV subtypes (H1–H10). Overall, we detected antibodies to NP in 24% (714/2,919) of geese. Antibodies to H3, H4, H5, and H6 subtypes predominated, with H5 being detected most frequently. A decrease in H5 HI antibody prevalence and titers was observed from 2009 to 2012. We also detected similar exposure pattern in Canada geese from New Jersey, Minnesota, Washington and Wisconsin. Based on the published literature, H3, H4, and H6 viruses are the most commonly reported IAVs from dabbling ducks. These results indicate that Canada geese also are frequently exposed to viruses of the same HA subtypes; however, the high prevalence of antibodies to H5 viruses was not expected as H5 IAVs are generally not well represented in reported isolates from ducks.

show abstract

Comparison of two commercial enzyme-linked immunosorbent assays for detection of Influenza A virus antibodies

Cited by 27 publications

References 14 publications

Surveillance of Influenza Viruses in Waterfowl Used As Decoys in Andalusia, Spain

Surveillance of Influenza Viruses in Waterfowl Used As Decoys in Andalusia, Spain

Evaluation of a commercial enzyme‐linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl

Subtype-specific influenza A virus antibodies in Canada geese (Branta canadensis)

Contact Info

Product

Resources

About