Comparison of two different biomaterials in the bone regeneration (15, 30 and 60 days) of critical defects in rats

Brassolatti, Patrícia; Bossini, Paulo Sérgio; Andrade, Ana Luiza Dias Leite de; Luna, Genoveva Lourdes Flores; Silva, Juliana Virginio da; Lopes, Luciana Almeida; Napolitano, Marcos Aurélio; Avó, Lucimar Retto da Silva de; Leal, Ângela M. O.; Aníbal, Fernanda de Freitas

doi:10.1590/acb360605

Cited by 5 publications

(5 citation statements)

References 50 publications

(62 reference statements)

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“…Furthermore, the biomaterial was more effective for bone regeneration than HA/PGLA without the addition of BLEED or in the absence of a scaffold. These findings highlight the need to understand the initial process of systemic inflammation in bone repair with the HA/PLGA/BLEED scaffold, since it showed good performance in healing at 15, 30, and 60 days 23 .…”

Section: Introductionmentioning

confidence: 81%

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Systemic and local inflammatory response after implantation of biomaterial in critical bone injuries

Brassolatti,

Castro,

Santos

et al. 2023

Acta Cir. Bras.

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Purpose: To evaluate inflammatory response in critical bone injuries after implantation of the biomaterial composed of hydroxyapatite (HA)/poly (lactic-coglycolic acid) (PLGA)/BLEED. Methods: Forty-eight male Wistar rats (280 ± 20 grams) were divided into two groups: control group (CG), in which the animals do not receive any type of treatment; and biomaterial group (BG), in which the animals received the HA/PLGA/BLEED scaffold. Critical bone injury was induced in the medial region of the skull calotte with the aid of a trephine drill 8 mm in diameter. The biomaterial was implanted in the form of 1.5-mm thick scaffolds. Serum and calotte were collected at one, three and seven days. Results: Biomaterial had a significant effect on the morphological structure of the bone, accelerating osteoblast activation within three days, without causing exacerbated systemic inflammation. In addition, quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that BG induced upregulation of osteogenic genes such as runt-related transcription factor 2, and stimulated genes of inflammatory pathways such as tumor necrosis factor-α, on the first day without overexpressing genes related to bone matrix degradation, such as tissue inhibitor of metalloproteinases-1 and matrix metalloproteinase-9. Conclusions: The HA/PLGA/BLEED ® association can be used as a bone graft to aid bone repair, as it is capable of modulating expression of important genes at this stage of the repair process.

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Section: Introductionmentioning

confidence: 81%

“…Subsequently, the vegetable polysaccharide paste commercially known as BLEED (developed and manufactured exclusively by DMC Import and Export of Equipment LTDA) was added. At the end of the process, the suspension was freeze-dried to obtain scaffolds with 1.5 mm of thickness and 8 mm in diameter 23 .…”

Section: Methodsmentioning

confidence: 99%

Systemic and local inflammatory response after implantation of biomaterial in critical bone injuries

Brassolatti,

Castro,

Santos

et al. 2023

Acta Cir. Bras.

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“…Throughout the experimental period (30 days), the animals were housed in the vivarium and received food and water ad libitum. This period is appropriated for bone regeneration of critical defects as can be seen elsewhere (Brassolatti et al, 2021;Efeoglu et al, 2009;Pripatnanont, Nuntanaranont, Vongvatcharanon, & Limlertmongkol, 2007).…”

Section: Biological Analysesmentioning

confidence: 99%

Bone regeneration in critical size calvarial defects using synthetic hydroxyapatite and mineralized bovine tendon base materials: a micro-computed tomography analysis

Amaral,

Viana,

Viana

et al. 2023

Acta Sci Technol

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This investigation used materials based on synthetic hydroxyapatite and mineralized bovine tendon as a framework for bone regeneration and evaluated the osteoconductivity of these materials in the calvaria of Wistar rats in comparison to Bio-Oss/Geistlich. Micro-computed tomography (µ-CT) analyses were performed non-invasively using three-dimensional image reconstruction to evaluate new bone formation; in addition, conventional histological analysis was used. The µ-CT results showed that Bio-Oss resulted in higher volume, density and bone percentage than the other materials. Based on the three-dimensional reconstructed images, the lowest resorption rates were observed in the Bio-Oss group, and the materials remained in larger quantities inside the defect at thirty days. In the synthetic hydroxyapatite group, intense resorption of the material and slight bone formation on the defect margins were noted, yielding an irregular edge. The mineralized bovine tendon group showed discrete new bone formation, and the material was fully resorbed. The Bio-Oss and synthetic hydroxyapatite groups yielded similar amounts of blood vessels and osteoblastic cells, and these were higher than the amounts found in the mineralized bovine tendon group. Synthetic hydroxyapatite was present within the defect and exhibited osteoconductive properties that were similar to the commercial brand, Bio-Oss. Mineralized bovine tendon did not exhibit good osteoconductivity and is contraindicated for maintaining bone space. Moreover, µ-CT yielded lower specificity; that is, µ-CT was not able to distinguish bone tissue from Bio-Oss, although it exhibited high sensitivity. Based on these results, it appears that synthetic hydroxyapatite has great potential for use in filling bone defects, unlike mineralized bovine tendon

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“…Several types of biomaterials have been used for the generation of bioartificial tissues by tissue engineering [ 1 , 2 , 3 , 4 ]. In general, biomaterials used in tissue engineering should be biocompatible, biodegradable and non-immunogenic, and should support cell attachment, growth and function [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Fibrin and Marine-Derived Agaroses for the Generation of Human Bioartificial Tissues: An Ex Vivo and In Vivo Study

et al. 2023

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Development of an ideal biomaterial for clinical use is one of the main objectives of current research in tissue engineering. Marine-origin polysaccharides, in particular agaroses, have been widely explored as scaffolds for tissue engineering. We previously developed a biomaterial based on a combination of agarose with fibrin, that was successfully translated to clinical practice. However, in search of novel biomaterials with improved physical and biological properties, we have now generated new fibrin-agarose (FA) biomaterials using 5 different types of agaroses at 4 different concentrations. First, we evaluated the cytotoxic effects and the biomechanical properties of these biomaterials. Then, each bioartificial tissue was grafted in vivo and histological, histochemical and immunohistochemical analyses were performed after 30 days. Ex vivo evaluation showed high biocompatibility and differences in their biomechanical properties. In vivo, FA tissues were biocompatible at the systemic and local levels, and histological analyses showed that biointegration was associated to a pro-regenerative process with M2-type CD206-positive macrophages. These results confirm the biocompatibility of FA biomaterials and support their clinical use for the generation of human tissues by tissue engineering, with the possibility of selecting specific agarose types and concentrations for applications requiring precise biomechanical properties and in vivo reabsorption times.

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Comparison of two different biomaterials in the bone regeneration (15, 30 and 60 days) of critical defects in rats

Cited by 5 publications

References 50 publications

Systemic and local inflammatory response after implantation of biomaterial in critical bone injuries

Systemic and local inflammatory response after implantation of biomaterial in critical bone injuries

Bone regeneration in critical size calvarial defects using synthetic hydroxyapatite and mineralized bovine tendon base materials: a micro-computed tomography analysis

Fibrin and Marine-Derived Agaroses for the Generation of Human Bioartificial Tissues: An Ex Vivo and In Vivo Study

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