Comparison of two different cryopreservation protocols for freezing goat semen

Küçük, Niyazi; Aksoy, Melih; Uçan, Uğur; Ahmad, Ejaz; Naseer, Zahid; Ceylan, Ahmet; Serin, İlker

doi:10.1016/j.cryobiol.2014.04.009

Cited by 32 publications

(22 citation statements)

References 33 publications

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“…Sperm DNA integrity was analyzed with comet assay procedure as reported previously (Küçük et al, 2014). Briefly, the slides were covered with 1% normal-melting-point agarose in phosphate buffered saline (PBS).…”

Section: Assessment Of Sperm Dna Integritymentioning

confidence: 99%

Effect of environmental heat stress on Kıvırcık ram sperm parameters

Küçük

Aksoy

2020

J Hellenic Vet Med Soc

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The relationship between environmental conditions and reproduction has been in sight of research. Therefore, there has been a growing interest in effect of climate change, global warming and subsequently environmental heat stress on gametes in last decades. Thus, the present study was designed to investigate the effect of environmental heat stress on Kıvırcık ram sperm parameters. In this study, semen samples (n=72) were collected from six mature rams during a 12-month study period. The temperature and relative humidity of animal box were controlled daily. The mean monthly temperature humidity index (THI) values were calculated to determine heat stress and control periods. According to the mean monthly THI values, the 36 of 72 ejaculates, which were collected between May and October, were classified as heat stress period samples. The others (n=36), which were collected between November and April, were considered as control period samples. The heat stress period sperm samples had lower sperm concentration, motility, viability, membrane integrity and higher abnormal sperm rate compared to control period sperm samples (P< 0.05). Semen volume, sperm DNA integrity and the ability of sperm to undergo acrosome reaction were detected similar between the heat stress and control periods. In conclusion, environmental heat stress was found deleterious for some sperm parameters in Kıvırcık rams.

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Section: Assessment Of Sperm Dna Integritymentioning

confidence: 99%

Effect of environmental heat stress on Kıvırcık ram sperm parameters

Küçük

Aksoy

2020

J Hellenic Vet Med Soc

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“…Em geral, os espermatozoides de pequenos ruminantes são mais sensíveis à criopreservação em comparação com a espécie bovina (1) . Além dessas características intrínsecas observadas nas diferentes espécies durante o processo de criopreservação, o choque térmico é normalmente associado ao estresse oxidativo, devido à geração de espécies reativas ao oxigênio (ROS), pelos espermatozoides mortos e oxigênio atmosférico.…”

Section: Introductionunclassified

Ácido Ascórbico No Diluidor Para Criopreservação De Sêmen Caprino

et al. 2017

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ResumoObjetivou-se determinar o efeito e o melhor nível de inclusão de ácido ascórbico em meio diluidor TRIS-Gema para criopreservação de sêmen caprino. Foram utilizados cinco ejaculados de três bodes da raça Anglo Nubiana. Cada ejaculado foi dividido em quatro alíquotas de 200 µL, compondo quatro tratamentos: um sem adição de ácido ascórbico (controle) e os demais com 0,0528; 0,1056 e 0,1584mg/mL de ácido ascórbico no diluidor TRIS-Gema. Avaliaram-se motilidade espermática progressiva e vigor espermático pós-diluição, pós-resfriamento e pós-descongelamento; integridade de membrana (HOST); integridade acrossomal e teste de termorresistência lento (TTR). Os dados foram submetidos à Análise de Regressão a 5% de probabilidade. Não houve diferença para motilidade progressiva e para vigor espermático, repectivamente, na pós-diluição; pós-resfriamento e pós-descongelamento, assim como para os testes complementares HOST e após 120 minutos do TTR, para motilidade e vigor, no pós-descongelamento (P>0,05). Houve comportamento quadrático com a inclusão do ácido ascórbico para integridade acrossomal (61,58%), com nível ótimo de 0,1006mg/mL de ácido ascórbico (P<0,05). A inclusão de 0,1006mg/mL de ácido ascórbico no diluidor TRIS-Gema melhorou a integridade acrossomal durante o processo de criopreservação, podendo ser uma alternativa na composição dos diluidores seminais caprinos. Palavras-chave: antioxidantes; espermatozoide; estresse oxidativo; TRIS-gema. AbstractThe objective of this study was to determine the optimal inclusion level of ascorbic acid in TRISyolk extenders for cryopreservation of goat semen. We used five ejaculates of three Anglo Nubian goats. Semen from each animal was divided into four 200μL aliquots, composing four treatments: no

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“…[32,33] However, it was observed that some researchers preferred directly spreading the agar-cell solution on the frosted microscope slide or gelbond. [34,35] The aim of using normal melting agar, frosted microscope slide or gelbond as a first layer might be to prevent possible loss of the cell-agar mixture during the lysis and electrophoresis procedures.…”

Section: Single Cell Gel Electrophoresismentioning

confidence: 99%

“…Several lysis solutions including variety of chemicals have been used for this process at different time intervals in various species. [33,[36][37][38][39] The process of electrophoresis: In this process, after the incubation of revealed sperm DNA in neutral or alkaline electrophoresis solution, the revealed sperm DNA is exposed to the electrophoresis in neutral or alkaline conditions. Different electrophoresis solutions, variety of electric powers and application times were used in the electrophoresis process in previous studies.…”

Section: The Lysis Processmentioning

confidence: 99%

“…COMET assay analysis programs or different computer-assisted image analysis programs were used for the assessment of the comet images in previous studies. [33,38,40,41] The COMET assay specific analysis programs might be the most objective method to be used for the assessment of comet images. Because it is possible to obtain many different parameters from these programs such as tail DNA (%), tail length (µm) and Olive tail moment which show the degree of the DNA damage in individual cells automatically.…”

Section: The Lysis Processmentioning

confidence: 99%

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Sperm DNA and detection of DNA fragmentations in sperm

Küçük¹

2018

Turkish Journal of Urology

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The questionable effectiveness of routine sperm parameters in determining male factor infertility problems and increasing the success rates of assisted reproductive techniques have led to the investigation of more detailed sperm parameters that could affect the male fertility and reproduction. Thus, the effects of different sperm parameters such as sperm DNA integrity was started to be investigated thanks to the previously described methods such as single cell gel electrophoresis (COMET) assay, sperm chromatin structure assay (SCSA), acridine orange test (AOT), terminal deoxynucleotidyl transferase-mediated deoxyuridine (TdT) triphosphate (dUTP) nick end labeling (TUNEL) assay and sperm chromatin dispersion (SCD) test. However, studying on sperm DNA might be very complex because the sperm DNA differs from the somatic cell DNA with its unique structure. Also, the sperm DNA undergoes many changes during spermatogenesis and it is condensed by being packaged tightly with different types and numbers of protamines in different species. Despite all these difficulties, these methods provide important information about the reasons and consequences of DNA damages in sperm and the effects of these damages on reproduction.

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Comparison of two different cryopreservation protocols for freezing goat semen

Cited by 32 publications

References 33 publications

Effect of environmental heat stress on Kıvırcık ram sperm parameters

Effect of environmental heat stress on Kıvırcık ram sperm parameters

Ácido Ascórbico No Diluidor Para Criopreservação De Sêmen Caprino

Sperm DNA and detection of DNA fragmentations in sperm

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