Comparison of two different models of sepsis induced by cecal ligation and puncture in rats

Song, Linlin; Zou, Yanjie; Cao, Zhongping

doi:10.1016/j.jss.2018.03.058

Cited by 12 publications

(11 citation statements)

References 21 publications

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“…Song et al [7] demonstrated that multiple organ dysfunction and death occurred at 6-12 h after CLP modeling. The present study verified, through modified CLP and FIP methods, that pathological damage of the intestine, lung, and heart was present as early as 6 h after modeling, and was exacerbated with time.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of 2 Rat Models for Sepsis Developed by Improved Cecal Ligation/Puncture or Feces Intraperitoneal-Injection

Fang

Gong

et al. 2020

Med Sci Monit

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Background: The aim of this study was to evaluate the clinical characteristics of 2 rat models of sepsis for improved cecal ligation/puncture (CLP) and feces intraperitoneal-injection (FIP), including systemic inflammation, organ dysfunction, and blood coagulation. Material/Methods: Sixty-two male SD rats were randomly divided into 3 groups: a normal control group (NC, n=6), a CLP group (n=28), and a FIP group (n=28). Ten rats each in the CLP and FIP groups were observed for 72-h mortality rate. The remaining 18 rats in each group were divided into 3 subgroups (n=6) according to their post-operation period (6, 12, and 24 h). Abdominal arterial blood was collected to determine the lactic acid (Lac) concentration, prothrombin time (PT), active partial prothrombin time (APTT), plasmic interleukin-6 (IL-6) level, and cardiac troponin (cTnI) level. The intestines, lung, and heart were collected for pathological examination. Results: The 72-h mortality rates in the CLP and FIP groups were 60% and 100%, respectively. The Lac level in both groups was significantly elevated at 6, 12, and 24 h after modeling. Compared with the NC group, PT in the CLP and FIP groups was prolonged at 12 and 24 h, and APTT was significantly prolonged at 6 h. IL-6 levels in the CLP and FIP groups peaked at 6 h. The cTnI level in the FIP group was significantly higher at 12 h after modeling compared with the NC group. The intestines, lung, and heart were pathologically damaged at 6 h, and this damage worsened over time. Conclusions: Both modeling methods induced sepsis in rats and closely mimicked the clinical conditions, but FIP was easier to establish and was more suitable for standardization.

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Section: Discussionmentioning

confidence: 99%

“…The CLP model was accepted as a typical model of sepsis, as it closely replicates the pathology and physiology of human sepsis [5,6]. However, extensive studies have proved certain limitations of the CLP model, such as instability and difficulty in establishing the model [7,8]. CASP is a model modified from CLP.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of 2 Rat Models for Sepsis Developed by Improved Cecal Ligation/Puncture or Feces Intraperitoneal-Injection

Fang

Gong

et al. 2020

Med Sci Monit

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“…O modelo CLP é considerado o padrão-ouro para estudos de sepse, mimetizando o curso clínico da sepse intra-abdominal (STARR et al, 2014;SONG;ZOU;CAO, 2018).…”

Section: Discussionunclassified

“…Contudo, ainda há alguns problemas difíceis de serem resolvidos e que afetam o prognóstico dos camundongos, como volume de fezes que vaza para a cavidade abdominal, e o fato de os focos de infecção serem facilmente envolvidos pelo omento e pelo tecido circundante (MAIER et al, 2004;POLI-DE-FIGUEIREDO et al, 2008;SONG;ZOU;CAO, 2018).…”

Section: Discussionunclassified

“…Além disso, uma diferença metodológica entre os dois experimentos foi a utilização do isoflurano a 2% em um deles para infusão da de marcadores semelhantes ao SOFA, e muitos dos artigos mais citados sobre sepse baseados em estudos de modelos animais não realizaram avaliação da disfunção orgânica, o que dificulta a comparabilidade dos estudos(LIBERT et al, 2019). No presente estudo, as concentrações dos marcadores de disfunção orgânica foram avaliadas 12 horas após a CLP, pois a disfunção orgânica ocorre geralmente de 6 a 12 horas após a indução da sepse, sendo causada, principalmente, pela resposta inflamatória sistêmica (ANGUS; VANRHODES et al, 2017;SONG;ZOU;CAO, 2018). Estudos prévios mostram que seria possível detectar alterações 12 horas após a indução da sepse com este modelo experimental Li et al (2018).…”

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Efeito da riboflavina na disfunção orgânica e na mortalidade por sepse grave e choque séptico: estudo experimental

Vale¹

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The effects of scopolamine on the survival time and microcirculation of septic shock rats

Song

Chu

Cao

2020

European Journal of Pharmaceutical Sciences

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Comparison of two different models of sepsis induced by cecal ligation and puncture in rats

Cited by 12 publications

References 21 publications

Evaluation of 2 Rat Models for Sepsis Developed by Improved Cecal Ligation/Puncture or Feces Intraperitoneal-Injection

Evaluation of 2 Rat Models for Sepsis Developed by Improved Cecal Ligation/Puncture or Feces Intraperitoneal-Injection

Efeito da riboflavina na disfunção orgânica e na mortalidade por sepse grave e choque séptico: estudo experimental

The effects of scopolamine on the survival time and microcirculation of septic shock rats

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