Comparison of Two Different Sampling Methods for Subgingival Plaque: Subgingival Paper Points or Mouthrinse Sample?

Abstract

There is no significant difference between MT4 and MSP for detection frequency of key pathogen A. actinomycetemcomitans. Key pathogens P. gingivalis, T. forsythia, T. denticola, P. micra, C. rectus, and E. nodatum show statistically higher detection frequencies with MT4.

Influence of periodontal treatment on subgingival and salivary microbiotas

“…To test the effect of periodontal treatment on salivary abundance of these periopathogens, we performed correlation analysis between salivary and subgingival levels of P. gingivalis , T. Forsythia , T. denticola , P. intermedia , P. micra , and F. alocis . Significant correlation on salivary and subgingival levels of P. gingivalis ( r = 0.60) and P. intermedia ( r = 0.78) was recorded at baseline, which agrees with previous cross‐sectional analysis, employing various contemporary methods for bacterial identification . Further, data demonstrated that salivary levels of periopathogens reflected subgingival alterations as an increase in correlation was noted from baseline to week 2 after periodontal treatment for all six periopathogens (Table ).…”

“…Primarily because of the ease and inexpensive nature of saliva sampling and analysis, saliva has been suggested as a substitute to local microbial sampling for monitoring of the oral microbiota . Differences in salivary bacterial profiles have been reported in patients with periodontitis compared to orally healthy controls, and several cross‐sectional studies have shown correlation between subgingival and salivary levels of specific periopathogens in periodontitis patients . However, red complex bacteria, that is, P. gingivalis, T. denticola , and T. forsythia , are also part of the tongue resident microbiota in orally healthy individuals, which is why salivary identification of periopathogens may not only reflect subgingival presence.…”

“…Furthermore, several cross‐sectional analyses have shown a positive correlation between subgingival and salivary levels of putative periopathogens such as P. gingivalis , T. forsythia , and T . denticola . It is therefore possible that salivary presence of periodontal pathogens may reflect dispersal of these bacteria from the subgingival microbiota into saliva.…”

Salivary microbiota and inflammation‐related proteins in patients with psoriasis

“…Notably, the majority of these bacterial species, including the proposed periopathogen P. gingivalis, were identified with higher relative abundance in patients with periodontitis. Salivary levels of P. gingivalis have been reported to associate with periodontitis (Damgaard et al, ), and previous studies have shown a positive correlation of salivary and subgingival levels of P. gingivalis (Belstrøm et al, ; Nickles, Scharf, Röllke, Dannewitz, & Eickholz, ) suggesting that the salivary microbiota reflects local bacterial alterations associated with periodontitis.…”

Doxycycline encapsulated in β-cyclodextrin for periodontitis: a clinical trial

“…2 Doxycycline (DOX) acts on Gram-positive and Gram-negative bacteria and on specific periodontal pathogens. 3 Its main antibiotic mechanism of action is the inhibition of protein synthesis. 4 It presents anti-inflammatory properties through direct inhibition of the activity of matrix metalloproteinases, which participate in periodontal tissue destruction 5 and are considered to be the most potent anti-collagenase Declaration of Interests: The authors certify that they have no commercial or associative interest that represents a conflict of interest in connection with the manuscript.…”