Comparison of two enzyme-linked immunosorbent assay (EIA) kits with immunofluorescence and isolation in cell culture for detection of respiratory syncytial virus (RSV)

Subbarao, Kanta; Whitehurst, Norma J.; Waner, Joseph L.

doi:10.1016/0732-8893(87)90054-x

Cited by 8 publications

(6 citation statements)

References 20 publications

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“…The combination of shell vial assay and cell culture detected RSV in 41 of 451 (9%) specimens lacking RSV antigen by ELISA. This was not an unexpected finding, and it is consistent with other reports of 4 to 27% false-negative RSV ELISA results in comparison with those of cell culture (2-4, 10,11,14,18,21,22). Since RSV diagnosis is important for infection control practice and for considering ribavirin therapy, we request cell culture backup of ELISA-negative specimens.…”

Section: Discussionsupporting

confidence: 89%

Rapid diagnosis of respiratory viral infections by using a shell vial assay and monoclonal antibody pool

et al. 1992

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We compared the detection of seven respiratory viruses by using a commercially available monoclonal antibody pool in a 2-day shell vial assay with that by using standard cell culture with respiratory syncytial virus (RSV) enzyme-linked immunosorbent assay (ELISA)-negative nasal secretions from hospitalized children. We found 179 respiratory virus isolates by either method in 675 specimens. Overall, the shell vial assay detected 147 of 179 (79%v) of the positives after 2 days; cell culture detected 148 of 179 (80%o) after a mean incubation period of 7.6 days (range, 1 to 14 days). The sensitivity of the shell vial assay was 78% for RSV, 94% for influenza B virus, 83% for adenovirus, and 80% for parainfluenza viruses. The sensitivity of the cell culture was 70% for RSV, 79%o for influenza B virus, 90%o for adenovirus, and 89%, for parainfluenza viruses. The 2-day shell vial assay allowed the detection of respiratory viruses in a clinically relevant time frame and rapidly detected RSV in specimens lacking RSV antigen by ELISA.

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Section: Discussionsupporting

confidence: 89%

Rapid diagnosis of respiratory viral infections by using a shell vial assay and monoclonal antibody pool

et al. 1992

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“…The specimens were diluted and retested, and the findings were included in the analysis. Of the 27 diluted specimens, 19 were positive and 8 were negative by Directigen RSV. Color intensity readings (1+ to 4+) were observed for 15 of the 19 positive specimens; 4 additional specimens were positive by Directigen RSV, but intensity readings were not recorded.…”

Section: Resultsmentioning

confidence: 99%

“…Hemadsorption tests employing guinea pig erythrocytes were performed at 4 and 22°C on PRMK cultures every 2 to 4 days. Virus isolates were identified by indirect IF, using monoclonal antibodies applied to cells scraped from culture tubes (19,22).…”

Section: Methodsmentioning

confidence: 99%

“…Cells were stained with a mixture of two monoclonal antibodies prepared and characterized in this laboratory and fluorescein-conjugated anti-mouse immunoglobulin G and immunoglobulin M as previously described (19,21); the antibodies are specific for a dimer of the fusion protein and a 27,000-molecular-weight (27K) to 35 K RSV protein.…”

Section: Methodsmentioning

confidence: 99%

“…Isolation in cell culture, direct immunofluorescence (IF), and enzyme immunoassays are currently the most common methods used for the identification of RSV (3,4,12,13,15,19). These procedures require laboratory support to various degrees.…”

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confidence: 99%

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Comparison of directigen RSV with viral isolation and direct immunofluorescence for the identification of respiratory syncytial virus

et al. 1990

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An enzyme immunoassay membrane test (Directigen RSV) for the detection of respiratory syncytial virus in clinical specimens was compared prospectively with isolation in cell culture and direct immunofluorescence (IF). A total of 315 nasopharyngeal wash specimens from pediatric patients were examined. Directigen RSV was 86.1% sensitive and 91.3% specific for specimens positive by isolation in cell culture and/or IF, with 88.6% agreement. The false-positive rate was 16%; 2 of 20 specimens giving false-positive reactions by Directigen RSV were true-positives by blocking assay. Twenty-seven specimens (8.5%) whose results were initially uninterpretable by Directigen RSV due to filtration difficulties were diluted and upon retesting produced acceptable results. Sixty-three viral isolates and/or IF identifications of virus antigens representing seven virus groups other than respiratory syncytial virus were also found; cross-reactions between Directigen RSV and other viruses were not observed. Directigen RSV will be useful as an immediate procedure and in facilities lacking a comprehensive virology laboratory.

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Evaluation of direct immunofluorescence, dot-blot enzyme immunoassay, and shell-vial culture for detection of respiratory syncytial virus in patients with bronchiolitis

Reina

Ros

Valle

et al. 1995

Eur. J. Clin. Microbiol. Infect. Dis.

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Comparison of two enzyme-linked immunosorbent assay (EIA) kits with immunofluorescence and isolation in cell culture for detection of respiratory syncytial virus (RSV)

Cited by 8 publications

References 20 publications

Rapid diagnosis of respiratory viral infections by using a shell vial assay and monoclonal antibody pool

Rapid diagnosis of respiratory viral infections by using a shell vial assay and monoclonal antibody pool

Comparison of directigen RSV with viral isolation and direct immunofluorescence for the identification of respiratory syncytial virus

Evaluation of direct immunofluorescence, dot-blot enzyme immunoassay, and shell-vial culture for detection of respiratory syncytial virus in patients with bronchiolitis

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