Summary:Objectives:To establish an intraocular pressure curve protocol that is safe for corneal health and determining if they detect harmful elevations of intraocular pressure outside normal clinic hours. To determine inter-user variability and if repeated measurements affect intraocular pressures.Methods: Dogs with glaucoma were included in the first part of the study in which intraocular pressures were measured using three protocols. Protocol 1 used applanation tonometry every two hours over 24h. Protocols 2 and 3 used applanation or rebound tonometry, respectively, and measured intraocular pressures every three hours over 30h. Sixty additional intraocular pressure curves from dogs with glaucoma and 20 from healthy dogs were then analysed for inter-user variability.
Results:128 Intraocular pressure curves were determined in 30 dogs. Protocol 1 resulted in one ulcer in five pressure curve measurements, Protocol 2 in one ulcer in 62 pressure curves, and Protocol 3 in no ulcers in 61. Elevated intraocular pressures were detected on 61 occasions, of which 26 developed outside normal clinic hours. Sixty-one additional intraocular pressure curves revealed that repeated measurements had no effect on intraocular pressure. Assessors had a significant variability in righteye but not left-eye readings.Conclusions: Protocol 3, using rebound tonometry every three hours for 30hr is safe and identified elevated intraocular pressures outside normal clinic hours in 12/30 (40%) of patients that single intraocular pressure measurement during consultation hours would not have identified. Intraocular pressure curves may be recommended for clinical practice and glaucoma studies.