Comparison of two homogeneous assays with a precipitation method and an ultracentrifugation method for the measurement of HDL-cholesterol

Arranz-Peña, M.L.; Tasende-Mata, Juan; Martín-Gil, F.J.

doi:10.1093/clinchem/44.12.2499

Cited by 31 publications

(10 citation statements)

References 21 publications

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“…TAG and cholesterol were assessed by the glucose oxidase-peroxidase (GOD-PAP) method ( 29 ) and the cholesterol oxidase-peroxidase (CHOD-PAP) method ( 30 ) , respectively. HDL-C and LDL-C were assessed by enzymic methods ( 31 , 32 ) , and apo A-I by immunoassay ( 33 ) . Thiobarbituric acid-reactive substances (TBARS) were quantified as described by Rueda-Jasso et al ( 34 ) using the malondialdehyde kit (Jiancheng Biotech Co., Nanjing, China).…”

Section: Methodsmentioning

confidence: 99%

Hypolipidaemic effects of fenofibrate and fasting in the herbivorous grass carp (Ctenopharyngodon idella) fed a high-fat diet

Clouet

Degrace

et al. 2008

Br J Nutr

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We investigated whether the hypolipidaemic effect of fenofibrate and fasting observed in most omnivorous mammals may also apply to herbivorous fish. Grass carp (Ctenopharyngodon idella) fed a high-fat (8 %) diet exhibited a marked increase in blood lipids and body fat after 6 weeks. They were then treated with fenofibrate (100 mg/kg body weight) in the same high-fat diet for 2 weeks, followed by fasting for 1 week. Plasma lipid concentration, body fat amount, fatty acid composition, plasma thiobarbituric acid-reactive substances and some parameters related to hepatic fatty acid oxidation were measured, and liver samples were stained for histological examination. Fenofibrate treatment decreased TAG and cholesterol concentrations in plasma, total lipids of the whole body and liver, and EPA and DHA contents in tissues. Further, a mobilisation of mesenteric fat concomitant with an increase in hepatic peroxisomal fatty acid oxidation and lipid peroxidation was observed. Compared with fenofibrate treatment, fasting decreased body weight and plasma TAG, but not plasma cholesterol. It also reduced the fat content of the whole body and increased the EPA and DHA contents in the liver and other tissues. Fatty acid oxidation was stimulated by fasting in mitochondria, but not in peroxisomes. These data suggest that fenofibrate and fasting regulate the lipid metabolism in grass carp through different metabolic pathways. The grass carp is moderately responsive to a fibrate derivative in comparison with the well-known excess responsiveness of the rat model, and so it could be used for the study of lipid abnormalities as a herbivorous model. Fibrate derivatives are widely used as normolipidaemic or hypolipidaemic drugs for the treatment of hypertriacylglycerolaemic and/or hypercholesterolaemic patients (1 -3) . As a lipid-lowering agent, fenofibrate has been shown to lower the concentration of plasma TAG and cholesterol (4,5) . Some mechanisms of clinical effects of fenofibrate have been identified, including interference with fatty acid (FA) synthesis, stimulation of hepatic FA oxidation, increase in lipoprotein lipolysis, inhibition of cholesterol biosynthesis, induction of hepatic uptake of cholesterol from plasma and increased elimination of cholesterol into bile as biliary acids (6) . Fenofibrate is also known to activate the transcriptional factor PPARa, which binds to the peroxisome proliferator response element in the regulatory region of target genes (7,8) . By this way, PPARa is known to induce enzymes involved in the regulation of lipid metabolism, particularly those related to mitochondrial and peroxisomal FA oxidation (9 -12) . It has also been suggested that fenofibrate regulates body weight (BW) and energy homeostasis by increasing FA oxidation (13) .Similarly to fibrate derivatives, fasting also activates PPARa and thereby regulates mitochondrial and peroxisomal FA oxidation activities (14,15) . The regulation of these pathways affects the lipid metabolism and was effectively demonstrated to modulate blood lipi...

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Section: Methodsmentioning

confidence: 99%

Hypolipidaemic effects of fenofibrate and fasting in the herbivorous grass carp (Ctenopharyngodon idella) fed a high-fat diet

Clouet

Degrace

et al. 2008

Br J Nutr

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“…Then cholesterol released by cholesterol esterase from either of the selected lipoproteins and treated with cholesterol oxidase produced H 2 O 2 . The amount of H 2 O 2 that is proportional to the amount of cholesterol was measured colorimetrically in the presence of 4-aminoantipyrine and N,N,-bis (4-sulfobutyl)-m-toluidine (Gordon et al 1977;Bachorik & Ross, 1995;Nakamura et al 1997;Arranz-Pena et al 1998). Thiobarbituric acid-reactive substances were estimated as described by Rueda-Jasso et al (2004) using a malondialdehyde kit (Jiancheng Biotech Co., Nanjing, China).…”

Section: Plasma Analysismentioning

confidence: 99%

Biochemical hepatic alterations and body lipid composition in the herbivorous grass carp (Ctenopharyngodon idella) fed high-fat diets

et al. 2006

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High-fat diets may have favourable effects on growth of some carnivorous fish because of the protein-sparing effect of lipids, but high-fat diets also exert some negative impacts on flesh quality. The goal of the study was therefore to determine the effects of fat-enriched diets in juvenile grass carp (Ctenopharyngodon idella) as a typical herbivorous fish on growth and possible lipid metabolism alterations. Three isonitrogenous diets containing 2, 6 or 10 % of a mixture of lard, maize oil and fish oil (1:1:1, by weight) were applied to fish for 8 weeks in a recirculation system. Data show that feeding diets with increasing lipid levels resulted in lowered feed intake, decreased growth and feed efficiency, and increased mesenteric fat tissue weight. Concomitantly, alteration of lipoprotein synthesis and greater level of lipid peroxidation were apparent in blood. In liver, muscle and mesenteric fat tissue, the percentages of a-linolenic acid and DHA were significantly increased or tended to increase with higher dietary lipid levels. Biochemical activity measurements performed on liver showed that, with the increase in dietary lipid level, there was a decrease in both mitochondrial and peroxisomal fatty acid oxidation capacities, which might contribute, at least in part, to the specific accumulation of a-linolenic acid and DHA into cells more active in membrane building. On the whole, grass carp have difficulty in energetically utilising excess dietary fat, especially when enriched in n-3 PUFA that are susceptible to peroxidation.

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“…Then cholesterol was released by cholesterol esterase and H 2 O 2 was produced by cholesterol oxidase. The level of H 2 O 2 , proportional to the level of cholesterol, was measured by colorimetric method in the presence of 4-aminoantipyrine and N,N,-bis (4-sulfobutyl) -m-toluidine ( Nakamura, 1997 ; Arranz-Peña et al., 1998 ).…”

Section: Methodsmentioning

confidence: 99%

Effects of dietary nicotinic acid supplementation on meat quality, carcass characteristics, lipid metabolism, and tibia parameters of Wulong geese

et al. 2021

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The purpose of this study was to evaluate the effects of nicotinic acid ( NA ) supplementation on the meat quality, carcass characteristics, lipid metabolism, and tibia parameters in Wulong geese. A total of 360 twenty-nine-day-old Wulong geese were randomly divided into 6 treatments, and each treatment included 6 pens with 10 birds per pen. Birds were fed a basal diet supplemented with 0, 20, 40, 60, 80, or 100 mg/kg NA for 12 wk. Dietary NA supplementation linearly decreased L* value and increased pH and water-holding capacity in the breast muscle ( P < 0.05). Increasing NA levels linearly and quadratically decreased shear force of breast muscle ( P < 0.001). Dietary NA supplementation linearly reduced the thickness of subcutaneous fat plus the skin and percentage of abdominal fat, and enhanced the width of intermuscular fat band ( P < 0.001). Dietary NA addition linearly and quadratically increased intramuscular fat ( IMF ) content ( P ≤ 0.001). Increasing NA levels decreased serum total cholesterol and low-density lipoprotein cholesterol levels and increased serum lipase activity and hepatic mRNA expression of lipoprotein lipase in a linear manner ( P < 0.05). There were linear and quadratic effects in serum triglycerides and high-density lipoprotein cholesterol ( HDL-C ) levels and malate dehydrogenase activity with the NA addition ( P < 0.05). Feeding the NA-supplemented-diets linearly increased tibia length, circumference, fat-free dry weight, and ash content ( P < 0.001). There were linear and quadratic increases in Ca and P contents with the NA supplementation ( P < 0.05). According to the quadratic regression analyses fitted to shear force, IMF content, serum triglycerides and HDL-C levels, and tibial Ca and P contents, the optimal dietary NA supplementation was 80 to 90 mg/kg. In conclusion, NA addition enhanced meat quality and IMF content, regulated lipid metabolism, and increased tibia quality of Wulong geese. The dosage of 80 mg/kg NA in Wulong geese aged 5 to 16 wk was recommended.

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Comparison of two homogeneous assays with a precipitation method and an ultracentrifugation method for the measurement of HDL-cholesterol

Cited by 31 publications

References 21 publications

Hypolipidaemic effects of fenofibrate and fasting in the herbivorous grass carp (Ctenopharyngodon idella) fed a high-fat diet

Hypolipidaemic effects of fenofibrate and fasting in the herbivorous grass carp (Ctenopharyngodon idella) fed a high-fat diet

Biochemical hepatic alterations and body lipid composition in the herbivorous grass carp (Ctenopharyngodon idella) fed high-fat diets

Effects of dietary nicotinic acid supplementation on meat quality, carcass characteristics, lipid metabolism, and tibia parameters of Wulong geese

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