Comparison of two identification and susceptibility test kits for<i>Ureaplasma</i>spp and<i>Mycoplasma hominis</i>in amniotic fluid of patients at high risk for intra-amniotic infection

Kusanovic, Juan Pedro; Vargas, Paula; Ferrer, Fernando; Díaz, Francisco; Córdova, Víctor; Martinovic, Carolina; Valdés, Rafael; Rosas, Alejandra; Luna, Daniela; Silva, Pablo G.; Silva, Karla; Nilo, María Elena; Silva, María José; Espejo, Eduardo; Zambrano, María Andrea; García, Jhon; Parra-Lara, Luis Gabriel; Escobar, María Fernanda

doi:10.1080/14767058.2019.1572742

Cited by 12 publications

(10 citation statements)

References 53 publications

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“…In our study, the concordance rate between IES and IST 2 was determined as 100% for both samples 95.3% and 96.2%, respectively, and reported the highest result for Mycoplasma IES [13]. In a study by Kusanovic et al (2020), the sensitivity of Mycoplasma IES, MYCOFAST RevolutioN and Mycoplasma IST 2 kits was determined as 100%, 96 and 95% for U. urealyticum, respectively, and it is consistent with the results we obtained from this study [16].…”

Section: Antibiotic Susceptibility Of the U Urealyticum Isolates With Ies Kitsupporting

confidence: 91%

Determination of Ureaplasma Urealyticum and Antibiotic Susceptibility in Sexually Active Women With Different Methods

ÖZTÜRK¹,

Yıldız²

2022

Ankara Universitesi Eczacilik Fakultesi Dergisi

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Objective: In this study, the presence of Ureaplasma urealyticum (U.urealyticum) in vaginal and urine samples taken from sexually active women was investigated and antibiotic susceptibilities were determined with culturing bacteria and using kits.Material and Method: The vaginal and urine samples taken from 110 women who applied to Başkent University Ankara Gynecology and Obstetrics Clinic were included in this study. U. urealyticum was investigated by the culture method with using two different test kits [Mycoplasma IES (IES-Autobio, China) and Mycoplasma IST2 (IST2) Biomereux, France] and Mycoplasma Agar. Antibiotic susceptibilities of isolates were also determined.Result and Discussion: We compared the results of (IES) and (IST2) used for U. urealyticum detection in 220 clinical samples. U. urealyticum was found to be positive in 82 (74,5%) by classical culture method. U. urealyticum was detected at a rate of 46.8% with the IST 2 and 53.6% with the IES. As a result of the comparison of culture method and commercial kits,it was determined that the IES kit provides a fast and accurate identification in the detection of U.urealyticum,a fast and reliable result in the detection of antibiotic resistance.A significant difference was found in the detection rate of U. urealyticum of the IES compared to the other commercial kit.It was observed that the detection rate of U. urealyticum with the kit from vaginal were significantly higher than urine samples.

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Section: Antibiotic Susceptibility Of the U Urealyticum Isolates With Ies Kitsupporting

confidence: 91%

Determination of Ureaplasma Urealyticum and Antibiotic Susceptibility in Sexually Active Women With Different Methods

ÖZTÜRK¹,

Yıldız²

2022

Ankara Universitesi Eczacilik Fakultesi Dergisi

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“…These mycoplasmas can establish persistent infections (Yavlovich et al, 2004), alter host cell physiology, modify apoptotic pathways (Chernov et al, 2015), induce the production of inflammatory substances (Benedetti et al, 2020), and result in cellular DNA damage and cancers (Zella et al, 2018). In addition, serious consequences like chronic obstructive pulmonary disease (COPD) (Feng et al, 2021) and infertility may occur (Kusanovic et al, 2020).…”

Section: Human Infectionmentioning

confidence: 99%

Mycoplasmas as Host Pantropic and Specific Pathogens: Clinical Implications, Gene Transfer, Virulence Factors, and Future Perspectives

Dawood

Algharib

Zhao

et al. 2022

Front. Cell. Infect. Microbiol.

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Mycoplasmas as economically important and pantropic pathogens can cause similar clinical diseases in different hosts by eluding host defense and establishing their niches despite their limited metabolic capacities. Besides, enormous undiscovered virulence has a fundamental role in the pathogenesis of pathogenic mycoplasmas. On the other hand, they are host-specific pathogens with some highly pathogenic members that can colonize a vast number of habitats. Reshuffling mycoplasmas genetic information and evolving rapidly is a way to avoid their host’s immune system. However, currently, only a few control measures exist against some mycoplasmosis which are far from satisfaction. This review aimed to provide an updated insight into the state of mycoplasmas as pathogens by summarizing and analyzing the comprehensive progress, current challenge, and future perspectives of mycoplasmas. It covers clinical implications of mycoplasmas in humans and domestic and wild animals, virulence-related factors, the process of gene transfer and its crucial prospects, the current application and future perspectives of nanotechnology for diagnosing and curing mycoplasmosis, Mycoplasma vaccination, and protective immunity. Several questions remain unanswered and are recommended to pay close attention to. The findings would be helpful to develop new strategies for basic and applied research on mycoplasmas and facilitate the control of mycoplasmosis for humans and various species of animals.

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“…Bacteriological culture has been considered the gold standard for the identification of genital mycoplasmas, but problems can arise from their fastidious growth requirements and a lack of reliable media. Commercialised kits for both culture and NAAT diagnosis are less laborious and have greater sensitivity and specificity compared with earlier in-house approaches 81 82. Sample integrity is also important and greatly influenced by sample collection methods (eg, type of swab, transport medium), transportation (eg, cold chain maintenance) and storage (eg, duration and temperature at which kept in long-term storage).…”

Section: Discussionmentioning

confidence: 99%

Adverse pregnancy and birth outcomes associated withMycoplasma hominis, Ureaplasma urealyticumandUreaplasma parvum: a systematic review and meta-analysis

et al. 2022

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ObjectivesMycoplasma hominis, Ureaplasma urealyticum and Ureaplasma parvum (genital mycoplasmas) commonly colonise the urogenital tract in pregnant women. This systematic review aims to investigate their role in adverse pregnancy and birth outcomes, alone or in combination with bacterial vaginosis (BV).MethodsWe searched Embase, Medline and CINAHL databases from January 1971 to February 2021. Eligible studies tested for any of the three genital mycoplasmas during pregnancy and reported on the primary outcome, preterm birth (PTB) and/or secondary outcomes low birth weight (LBW), premature rupture of membranes (PROM), spontaneous abortion (SA) and/or perinatal or neonatal death (PND).Two reviewers independently screened titles and abstracts, read potentially eligible full texts and extracted data. Two reviewers independently assessed risks of bias using published checklists. Random effects meta-analysis was used to estimate summary ORs (with 95% CIs and prediction intervals). Multivariable and stratified analyses were synthesised descriptively.ResultsOf 57/1194 included studies, 39 were from high-income countries. In meta-analysis of unadjusted ORs, M. hominis was associated with PTB (OR 1.87, 95% CI 1.49 to 2.34), PROM, LBW and PND but not SA. U. urealyticum was associated with PTB (OR 1.84, 95% CI 1.34 to 2.55), PROM, LBW, SA and PND. U. parvum was associated with PTB (1.60, 95% CI 1.12 to 2.30), PROM and SA. Nine of 57 studies reported any multivariable analysis. In two studies, analyses stratified by BV status showed that M. hominis and U. parvum were more strongly associated with PTB in the presence than in the absence of BV. The most frequent source of bias was a failure to control for confounding.ConclusionsThe currently available literature does not allow conclusions about the role of mycoplasmas in adverse pregnancy and birth outcomes, alone or with coexisting BV. Future studies that consider genital mycoplasmas in the context of the vaginal microbiome are needed.PROSPERO registration numberCRD42016050962.

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Comparison of two identification and susceptibility test kits forUreaplasmaspp andMycoplasma hominisin amniotic fluid of patients at high risk for intra-amniotic infection

Cited by 12 publications

References 53 publications

Determination of Ureaplasma Urealyticum and Antibiotic Susceptibility in Sexually Active Women With Different Methods

Determination of Ureaplasma Urealyticum and Antibiotic Susceptibility in Sexually Active Women With Different Methods

Mycoplasmas as Host Pantropic and Specific Pathogens: Clinical Implications, Gene Transfer, Virulence Factors, and Future Perspectives

Adverse pregnancy and birth outcomes associated withMycoplasma hominis, Ureaplasma urealyticumandUreaplasma parvum: a systematic review and meta-analysis

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