Comparison of two media for the isolation of thermophilic Campylobacters from waste waters of different quality

Jacob, Jens; Stelzer, W.

doi:10.1016/s0232-4393(11)80361-6

Cited by 4 publications

(2 citation statements)

References 23 publications

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“…In addition, in agreement with data reported by most investigators, we also observed the presence of a large number of viable but not cultivable Campylobacter cells (Rollins & Colwell 1986, Carter et al 1987, Höller 1988, Humphrey 1989, Pokorny 1990, Jacob & Stelzer 1992, Höller & Schomakers-Revaka 1994.…”

Section: Resultssupporting

confidence: 92%

Diversity of Campylobacter Isolates from Three Activated Sludge Systems

Lauria-Filgueiras

Hofer

1998

Mem. Inst. Oswaldo Cruz

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Section: Resultssupporting

confidence: 92%

Diversity of Campylobacter Isolates from Three Activated Sludge Systems

Lauria-Filgueiras

Hofer

1998

Mem. Inst. Oswaldo Cruz

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“…is assumed to originate from animal faeces, agricultural leaks or wastewater contamination. The organism can indeed be recovered from untreated wastewater [ 9 , 15 , 16 , 17 , 18 ]. However, the detection methods are rather insensitive and elaborate.…”

Section: Introductionmentioning

confidence: 99%

Method Comparison for Enhanced Recovery, Isolation and Qualitative Detection of C. jejuni and C. coli from Wastewater Effluent Samples

Ugarte‐Ruiz

Florez‐Cuadrado

Wassenaar

et al. 2015

IJERPH

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Seeking a sensitive protocol, culture-dependent methods were compared to detect thermophilic Campylobacter species in untreated urban effluents. We evaluated various combinations of selective media, with and without an enrichment steps, as well as an extra filtration step. Culture-independent real-time quantitative PCR was also included and all detected isolates underwent antimicrobial susceptibility testing. All tested water samples contained Campylobacter DNA, but only 64% were positive after culture. Although enrichment using Preston broth resulted in better recovery of potentially stressed Campylobacter than Bolton or Campyfood broth (CFB), there was no significant increase in efficiency compared to direct plating. The type of selective agar media used, on the other hand, had a significant effect, with CASA plates performing better than mCCDA or CFA ones. Inclusion of an enrichment step increased the ratio of C. coli vs. C. jejuni being isolated. Resistances against all antimicrobials tested were observed in C. coli, but fewer instances of resistance were found in C. jejuni isolates. Whether this difference was the result of selection during the enrichment step could not be determined. The presence of Campylobacter in urban effluents can be considered as a valuable proxy for Campylobacter populations present in urban environments.

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Detection and occurrence of waterborne bacterial and viral pathogens

Black¹,

Finch²

1993

Water Environment Research

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Comparison of two media for the isolation of thermophilic Campylobacters from waste waters of different quality

Cited by 4 publications

References 23 publications

Diversity of Campylobacter Isolates from Three Activated Sludge Systems

Diversity of Campylobacter Isolates from Three Activated Sludge Systems

Method Comparison for Enhanced Recovery, Isolation and Qualitative Detection of C. jejuni and C. coli from Wastewater Effluent Samples

Detection and occurrence of waterborne bacterial and viral pathogens

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