Comparison of two methods of encapsulation of an oligonucleotide into poly(d,l-lactic acid) particles

Delié, Florence; Berton, Myriam; Allémann, Éric; Gurny, Robert

doi:10.1016/s0378-5173(00)00627-x

Cited by 47 publications

(25 citation statements)

References 16 publications

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“…The entrapment efficiencies of CEL in the particles were found to be in the range 88 -99%. These values are significantly higher than the values typically reported for other encapsulation methods such as emulsion methods [59,60]. Although differences in EE were observed, there did not seem to be any correlation between the EE and the processing conditions.…”

Section: System Yield and Entrapment Efficiencycontrasting

confidence: 63%

Release profile and characteristics of electrosprayed particles for oral delivery of a practically insoluble drug

Bohr

Kristensen²,

Dyas³

et al. 2012

J. R. Soc. Interface.

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acid) (PLGA) microspheres containing celecoxib were prepared via electrospraying, and the influence of three processing parameters namely flow rate, solute concentration and drug loading, on the physico-chemical properties of the particles and the drug-release profile was studied. Microspheres with diameters between 2 and 8 mm were produced and a near-monodisperse size distribution was achieved ( polydispersivity indices of 6-12%). Further, the inner structure of the particles showed that the internal porosity of the particles increased with increasing solvent concentration. X-ray powder diffraction (XRPD) analysis indicated that the drug was amorphous and remained stable after eight months of storage. Drug release was studied in USP 2 (United States Pharmacopeia Dissolution Apparatus 2) dissolution chambers, and differences in release profiles were observed depending on the parametric values. Changes in release rate were found to be directly related to the influence of the studied parameters on particle size and porosity. The results indicate that electrospraying is an attractive technique for producing drug-loaded microspheres that can be tailored towards an intended drug-delivery application. Compared with the more conventional spray-drying process, it provides better control of particle characteristics and less aggregation during particle formation. In particular, this study demonstrated its suitability for preparing capsules in which the drug is molecularly dispersed and released in a sustained manner to facilitate improved bioavailability.

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Section: System Yield and Entrapment Efficiencycontrasting

confidence: 63%

Release profile and characteristics of electrosprayed particles for oral delivery of a practically insoluble drug

Bohr

Kristensen²,

Dyas³

et al. 2012

J. R. Soc. Interface.

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show abstract

“…The microencapsulation of a hydrophilic molecule by this technique is a complex process involving several factors. High ODN loadings are often obtained by using cationic compounds that complex ODNs and contribute to improve emulsion stability, although their toxicity remains a crucial problem [20,27]. In our previous studies, microspheres containing a phosphorothioate ODN, prepared by two different PLGA types, exhibited a higher ODN encapsulation efficiency when the inherent viscosity of the polymer was increased [28].…”

Section: Discussionmentioning

confidence: 96%

Enhanced intracellular uptake and inhibition of NF‐κB activation by decoy oligonucleotide released from PLGA microspheres

Rosa

Maiuri

Ungaro

et al. 2005

The Journal of Gene Medicine

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“…The primary emulsion is then poured into a large volume of liquid paraffin. The double emulsion technique has fairly good encapsulation efficiency for hydrophilic compounds; however, particle size is usually larger than that of single emulsion technique (5). Once the particles are formed, a purification step is needed.…”

Section: Introductionmentioning

confidence: 99%

Development of pH-sensitive Insulin Nanoparticles using Eudragit L100-55 and Chitosan with Different Molecular Weights

et al. 2010

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Abstract. Insulin is a polypeptide hormone and usually administered for treatment of diabetic patients subcutaneously. The aim of this study was to investigate the efficiency of enteric nanoparticles for oral delivery of insulin. Nanoparticles were formed by complex coacervation method using chitosan of various molecular weights. Nanoparticles were characterized by drug loading efficiency determination, particle size analysis, Scanning Electron Microscopy (SEM), Zeta potential and CD spectroscopy (Circular Dichrosim). The in vitro release studies were performed at pH 1.2 and 7.4. The drug loaded nanoparticles showed 3.38% of entrapment, loading efficiency of 30.56% and mean particle size of 199 nm. SEM studies showed that the nanoparticles are non-spherical. Zeta potential increased with increasing molecular weight of chitosan. The CD spectroscopy profiles indicated that the nano-encapsulation process did not significantly disrupt the internal structure of insulin; additionally, pH-sensitivity of nanoparticles was preserved and the insulin release was pH-dependent. These results suggest that the complex coacervation process using chitosan and Eudragit L100-55 polymers may provide a useful approach for entrapment of hydrophilic polypeptides without affecting their conformation.

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Comparison of two methods of encapsulation of an oligonucleotide into poly(d,l-lactic acid) particles

Cited by 47 publications

References 16 publications

Release profile and characteristics of electrosprayed particles for oral delivery of a practically insoluble drug

Release profile and characteristics of electrosprayed particles for oral delivery of a practically insoluble drug

Enhanced intracellular uptake and inhibition of NF‐κB activation by decoy oligonucleotide released from PLGA microspheres

Development of pH-sensitive Insulin Nanoparticles using Eudragit L100-55 and Chitosan with Different Molecular Weights

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