Comparison of two models of inflammatory bowel disease in rats

Cătană, Cristina Sorina; Magdaş, Cristian; Tabaran, Flaviu; Craciun, Cristina; Deak, Georgiana; Magdaş, Virginia Ana; Cozma, Vasile; Gherman, Călin Mircea; Berindan‐Neagoe, Ioana; Dumitraşcu, Dan L.

doi:10.17219/acem/69134

Cited by 21 publications

(15 citation statements)

References 30 publications

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“…The literature data and our study showed that in terms of morphology and histopathology, the TNBS model of colon inflammation used in the present study is adequate for early stage of Crohn's disease (CD) in humans [18,28], data not published]. The rectal administration of trinitrobenzene sulfonic acid (TNBS) results in a strictly localized trans-wall inflammation causing local immune response with dense lymphocyte infiltration and secretion of proinflammatory cytokines in the whole wall of the large intestine [29,30]. The occurrence of typical for CD features in the large intestine of rats included in our experiment was confirmed by histological analysis of the pathologically altered sections of the large intestine (rectum) and the analysis of the concentration of proinflammatory cytokines performed on the same material.…”

Section: Discussionmentioning

confidence: 92%

Time-Dependent Indirect Antioxidative Effects of Oat Beta-Glucans on Peripheral Blood Parameters in the Animal Model of Colon Inflammation

et al. 2020

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Background: Oat beta-glucans are polysaccharides, belonging to soluble fiber fraction, that show a wide spectrum of biological activity. The aim of this study was to evaluate the time-dependent antioxidative effect of chemically pure oat beta-glucan fractions, characterized by different molar mass, which were fed to animals with early stage of 2,4,6-trinitrobenzene sulfonic acid (TNBS) - induced colitis. Methods: The study was conducted on 150 adult male Sprague Dawley rats assigned to two groups—healthy control (H) and colitis (C) with colon inflammation induced by per rectum administration of TNBS. The animals from both groups were divided into 3 nutritional subgroups, receiving for 3, 7 or 21 days AIN-93M feed without beta-glucan (βG−) or with 1% (w/w) low molar mass oat beta-glucan (βGl+) or 1% (w/w) high molar mass oat beta-glucan (βGh+). After 3, 7 and 21 days, the animals were euthanized, peripheral blood was collected from the heart for further analysis. Results: The results of analyses performed on blood samples showed small changes in lymphocytes count and red blood cell parameters such as the number of red blood cell, mean corpuscular hemoglobin concentration and mean corpuscular volume (RBC, MCHC, MCV respectively) as well as normalization of antioxidant potential accompanying moderate inflammatory state of colon mucosa and submucosa. Conclusion: Oat beta-glucans exert an indirect antioxidant effect in animals with TNBS-induced colitis, with greater effectiveness in removing systemic effects of colon inflammation found for low molar mass oat beta-glucan.

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Section: Discussionmentioning

confidence: 92%

Time-Dependent Indirect Antioxidative Effects of Oat Beta-Glucans on Peripheral Blood Parameters in the Animal Model of Colon Inflammation

et al. 2020

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“…The use of the TNBS-induced colitis model allowed assessing immunological parameters at various stages of development of inflammatory lesions in the colon of rats, which corresponded to CD phases characterized by different severity levels in humans. Colitis induced by rectal administration of an ethanolic solution of TNBS, which is well described in the literature [ 19 , 20 ], causes transmural changes with clinical symptoms and a histological presentation characteristic of CD in humans [ 21 , 22 ]. This colitis model is also suitable for studying cell signaling pathways [ 23 ] and for analyzing a wide spectrum of cytokines [ 21 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…Three days after TNBS administration, rats fed the feed without oat beta-glucan supplementation developed local acute inflammation, which is a characteristic of the active phase of CD [ 23 ]. Histological evaluation of the colon confirmed extensive transmural inflammation, including inflammation of the mucosa and submucosal layers, which are characteristics of CD [ 22 ]. Seven days after TNBS administration, these symptoms improved, indicating the onset of inflammatory remission.…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Activity of Oat Beta-Glucans in a Crohn’s Disease Model: Time- and Molar Mass-Dependent Effects

Żyła

Dziendzikowska

Kamola

et al. 2021

IJMS

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Background: The incidence of Crohn’s disease (CD) is increasing worldwide, and it has currently become a serious public health issue in society. The treatment of CD continues throughout a patient’s lifetime, and therefore, it is necessary to develop new, effective treatment methods, including dietotherapy. The present study aimed to determine the effects of consumption of oat beta-glucans with different molar mass on colon inflammation (colitis) in the early stages of 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced CD in an animal model. Methods: Sprague–Dawley rats (control and TNBS-induced CD) were divided into three dietary groups and fed for 3 days (reflecting acute inflammation) or 7 days (reflecting remission) with a feed containing 1% low (βGl) or high (βGh) molar mass oat beta-glucan or a feed without this polysaccharide. The level of colon inflammatory markers and the expression of cytokines and their receptor genes were measured by ELISA and RT-PCR methods, respectively. Results: Acute inflammation or remission (3 or 7 days after TNBS administration, respectively) stages of experimentally induced CD were characterized by an increase in the level of inflammatory markers (IL-1, IL-6, IL-10, IL-12, TNF-α, CRP, MPO, COX, and PGE2) and the disruption of some cytokine signaling pathways as well as macro- and microscopic changes of colon tissue. The consumption of oat beta-glucans reduced the level of inflammatory markers and recovered the signaling pathways and histological changes, with stronger effects of βGl after 7 days of colitis. Conclusions: Dietary oat beta-glucans can reduce colitis at the molecular and organ level and accelerate CD remission.

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“…In this study, we investigated the effect of oat beta-glucans on experimentally TNBS-induced colitis, which is a well-described intestinal inflammation model with predominantly Crohn’s disease-like features due to the transmural character of the inflammation. Intrarectal administration of TNBS dissolved in 50% ethanol results in the induction of colitis, which mimics human Crohn’s disease (CD) [12,13]. Ethanol disrupts the colon epithelial barrier, enabling the interaction of TNBS with colon’s tissue proteins, which cause transmural inflammation.…”

Section: Discussionmentioning

confidence: 99%

Beneficial Effects of Oat Beta-Glucan Dietary Supplementation in Colitis Depend on Its Molecular Weight

et al. 2019

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Background: Inflammatory bowel diseases are an important health problem. Therefore, the aim of the present study was to compare the impact of isolated oat beta-glucan fractions of low and high molecular weight, taken as dietary supplementation, on inflammatory markers in the colitis model. Methods: Two groups of Sprague–Dawley rats—control and with experimentally induced colitis—were subsequently divided into three subgroups and fed over 21 days feed supplemented with 1% of low (βGl) or high (βGh) molecular weight oat beta-glucan fraction or feed without supplementation. The level of colon inflammatory markers, cytokines, and their receptors’ genes expressions and immune cells numbers were measured by ELISA, RT-PCR, and by flow cytometry methods, respectively. Results: The results showed moderate inflammation affecting the colon mucosa and submucosa, with significant changes in the number of lymphocytes in the colon tissue, elevated cytokines and eicosanoid levels, as well as disruption of the main cytokine and chemokine cell signaling pathways in colitis rats. Beta-glucans supplementation caused a reverse in the percentage of lymphocytes with stronger effects of βGh and reduction of the levels of the inflammatory markers, and improvement of cytokine and chemokine signaling pathways with stronger effects of βGl supplementation. Conclusions: The results indicate the therapeutic effect of dietary oat beta-glucan supplementation in the colitis in evident relation to the molecular weight of polymer.

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Comparison of two models of inflammatory bowel disease in rats

Abstract: The experimental DSSor TNBS-induced bowel inflammation used in this study corresponds to human IBD and is reproducible with characteristics indicative of acute inflammation in the case of the protocols mentioned.

Cited by 21 publications

References 30 publications

Time-Dependent Indirect Antioxidative Effects of Oat Beta-Glucans on Peripheral Blood Parameters in the Animal Model of Colon Inflammation

Time-Dependent Indirect Antioxidative Effects of Oat Beta-Glucans on Peripheral Blood Parameters in the Animal Model of Colon Inflammation

Anti-Inflammatory Activity of Oat Beta-Glucans in a Crohn’s Disease Model: Time- and Molar Mass-Dependent Effects

Beneficial Effects of Oat Beta-Glucan Dietary Supplementation in Colitis Depend on Its Molecular Weight

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