Comparison of two multiplex arrays in the diagnostics of allergy

Bojcukova, Jana; Vlas, Tomas; Forstenlechner, Peter; Panzner, Petr

doi:10.1186/s13601-019-0270-y

Cited by 43 publications

(32 citation statements)

References 10 publications

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“…Correlations beyond 30 kUA/L between ALEX and ImmunoCAP require individual assessment of each allergen. For inhalant allergens, additional comparison studies have found significant correlations between ALEX and ISAC within three cohorts, 13,33,34 supporting the potential utility of these conversion methods for other allergens.…”

Section: Discussionmentioning

confidence: 84%

Modeling the conversion between specific IgE test platforms for nut allergens in children and adolescents

Hoang

Çelik

Lupinek

et al. 2020

Allergy

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Background Multiplex tests allow for measurement of allergen‐specific IgE responses to multiple extracts and molecular allergens and have several advantages for large cohort studies. Due to significant methodological differences, test systems are difficult to integrate in meta‐analyses/systematic reviews since there is a lack of datasets with direct comparison. We aimed to create models for statistical integration of allergen‐specific IgE to peanut/tree nut allergens from three IgE test platforms. Methods Plasma from Canadian and Austrian children/adolescents with peanut/tree nut sensitization and a cohort of sensitized, high‐risk, pre‐school asthmatics (total n = 166) were measured with three R&D multiplex IgE test platforms: Allergy Explorer version 1 (ALEX) (Macro Array Dx), MeDALL‐chip (Mechanisms of Development of Allergy) (Thermo Fisher), and EUROLINE (EUROIMMUN). Skin prick test (n = 51) and ImmunoCAP (Thermo Fisher) (n = 62) results for extracts were available in a subset. Regression models (Multivariate Adaptive Regression Splines, local polynomial regression) were applied if >30% of samples were positive to the allergen. Intra‐test correlations between PR‐10 and nsLTP allergens were assessed. Results Using two regression methods, we demonstrated the ability to model allergen‐specific relationships with acceptable measures of fit (r2 = 94%‐56%) for peanut and tree nut sIgE testing at the extract and molecular‐level, in order from highest to lowest: Ara h 2, Ara h 6, Jug r 1, Ana o 3, Ara h 1, Jug r 2, and Cor a 9. Conclusion Our models support the notion that quantitative conversion is possible between sIgE multiplex platforms for extracts and molecular allergens and may provide options to aggregate data for future meta‐analysis.

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Section: Discussionmentioning

confidence: 84%

Modeling the conversion between specific IgE test platforms for nut allergens in children and adolescents

Hoang

Çelik

Lupinek

et al. 2020

Allergy

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“…In brief, ALEX is a multiplex array containing 282 reagents (157 extractive allergens and 125 molecular components). The different allergens and components were coupled onto polystyrene nano-beads, and then, the allergen beads were deposited on a nitrocellulose membrane, as published elsewhere [26]. Total IgE levels were expressed in international units per unit volume (IU/mL), and sIgE levels were expressed in kU A /L.…”

Section: Methodsmentioning

confidence: 99%

Mite Molecular Profile in the Th2-Polarized Moderate-to-Severe Persistent Asthma Endotype Subjected to High Allergen Exposure

González‐Pérez

Poza‐Guedes

Pineda

et al. 2020

Int Arch Allergy Immunol

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Background: The association among the IgE responses to prevailing groups of house dust mite (HDM) allergens in the concurrent asthma phenotypes has not been determined. Objective: The aim of the present study lays on a component-resolved diagnosis (CRD) model to investigate the mite molecular signature in subjects with type-2 inflammation asthma. Methods: We selected patients showing a clinically relevant sensitization to HDMs with moderate-to-severe persistent asthma. Skin prick test (SPT) with standardized mite extracts, a broad customized CRD serum sIgE panel including 9 Dermatophagoides pteronyssinus allergens and the related protein allergenic characterization, was investigated in all serum samples. Results: Ninety out of 93 (96.77%) patients with a positive SPT to HDM showed a concordant sIgE (≥0.35 kUA/L) to the crude extract of D. pteronyssinus. Major allergens (Der p 2, Der p 23, and Der p 1) were present in >70% of all subjects, with mid-tier allergens (Der p 5, Der p 7, and Der p 21) reaching up to 51% in the present cohort. A complex pleomorphic repertoire of HDM molecules recognized by IgE was depicted, including 38 distinct profiles. Conclusions and Clinical Relevance: The proposed CRD panel approach, containing the most prevalent HDM allergens, appeared to be sufficient to obtain a precise D. pteronyssinus molecular diagnosis in asthmatics with a climate-dependent high-mite allergen exposure and coexisting sensitization. A dominant role of both major and mid-tier allergens has been confirmed in moderate and severe persistent asthmatics with the preponderant Th2-high endotype.

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“…The published data indicate that the results of the various in vitro tests are not comparable or interchangeable [23,[31][32][33]. Although there may be some degree of correlation between tests, the results are not interchangeable through conversion factors due to the lack of units referred to a common standard [23,[33][34][35][36][37].…”

Section: Considerations Before Switching To a Different In Vitro Assamentioning

confidence: 99%

VALIDA project: Validation of allergy in vitro diagnostics assays (Tools and recommendations for the assessment of in vitro tests in the diagnosis of allergy)

Casas

Esteban

González‐Muñoz

et al. 2020

Advances in Laboratory Medicine / Avances en Medicina De Laboratorio

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In vitro allergen-specific immunoglobulin E (IgE) detection and quantification tests are routinely performed in clinical laboratories to diagnose patients with a suspected allergy. Numerous commercial assays are available to test for allergies, but the results can vary widely, thereby influencing both diagnosis and treatment. Given the challenges posed by differences in the various assays for in vitro determination of specific IgE, a group of experts has compiled in a document a series of recommendations on the implications that the use of a certain in vitro technique may have and the impact on the management of the allergic patient that the differences between the various techniques represent. The reading and analysis of this consensus document will help to understand the implications of the change of in vitro diagnostic method in the management of the patient with allergy, in the quality of life and in the socioeconomic costs associated with the disease.

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Comparison of two multiplex arrays in the diagnostics of allergy

Cited by 43 publications

References 10 publications

Modeling the conversion between specific IgE test platforms for nut allergens in children and adolescents

Modeling the conversion between specific IgE test platforms for nut allergens in children and adolescents

Mite Molecular Profile in the Th2-Polarized Moderate-to-Severe Persistent Asthma Endotype Subjected to High Allergen Exposure

VALIDA project: Validation of allergy in vitro diagnostics assays (Tools and recommendations for the assessment of in vitro tests in the diagnosis of allergy)

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