Comparison of two sampling techniques to assess quantity and distribution of<i>Malassezia</i>yeasts on the skin of Basset Hounds

Bensignor, Emmanuel; Jankowski, F.; Seewald, Wolfgang; Touati, François-Olivier; Deville, Manjula; Guillot, Jacques

doi:10.1046/j.1365-3164.2002.00308.x

Cited by 35 publications

(52 citation statements)

References 13 publications

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“…31 Various methods have been used to collect material for cytological and cultural examinations of Malassezia species. Cytological techniques include impression methods using glass slides, cotton swabs, skin scraping, and tape strip preparation, 1,23,32 whereas mycological cultures may be obtained from cotton swabs or directly with contact plates. 2 Some of these techniques may be less sensitive than others, but none has yet been accepted as a standard.…”

Section: Discussionmentioning

confidence: 99%

“…2 Some of these techniques may be less sensitive than others, but none has yet been accepted as a standard. 1,2,6,23,32 On the basis of the empirical evaluation of this study, cotton swabs were considered to be the most reliable technique because they may be used for both cytological examination and culture and are easy to use. It is well known that cytological examinations of the ear or skin of dogs detect a small number of commensal organisms.…”

Section: Discussionmentioning

confidence: 99%

“…16 The frequency and population size of M. pachydermatis vary markedly among different anatomical sites in dogs. 1,2,5,6,18,23 These parameters are probably affected by the presence of concomitant seborrheic, atopic, and allergic dermatitis in dogs with skin disorders or Crossbreeds 26 9 4 8 9 30 56 Boxer 3 1 4 4 Breton 3 3 3 Cocker Spaniel 1 1 Dalmatian 1 1 1 German Shepherd 2 3 1 6 10 12 Setter 2 1 1 3 Siberian Husky 3 3 3 Yorkshire 1 3 3 4 Total 33 15 6 9 24 54 87 otitis (or both). 2,3,5 The frequency and population size of M. pachydermatis also depend on the dog breed.…”

Section: Introductionmentioning

confidence: 99%

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Frequency, Body Distribution, and Population Size of Malassezia Species in Healthy Dogs and in Dogs with Localized Cutaneous Lesions

et al. 2005

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Abstract. Malassezia species are commensal organisms of human and animal skin that occasionally act as opportunistic pathogens. The lipid-dependent species are associated with human skin disorders, whereas the non-lipid-dependent species (Malassezia pachydermatis) is considered as an opportunistic secondary pathogen affecting the canine skin surface and ear canal. This study evaluated the relationship between Malassezia yeasts, their population size, and the occurrence of skin lesions from healthy and skin-diseased dogs. The efficiency of cytological examination and fungal culture for Malassezia detection was also evaluated. From March 2002 to July 2003, 33 healthy dogs and 54 dogs with pruritic localized skin diseases were examined; skin swabs (1218) were collected from 7 anatomical sites for culture and cytological examination. Malassezia prevalence according to anatomical site and the agreement between cytological results and fungal cultures were statistically analyzed. Differences in mean colony forming unit counts between positive healthy and diseased dogs were evaluated using the Bonferroni test for post hoc pair-wise comparisons. In healthy dogs, Malassezia yeasts were most frequently isolated in the perianal and perioral areas. The frequency of isolation and population size of Malassezia species were higher in dogs with localized dermatitis, especially in affected areas, indicating a role for Malassezia in the occurrence of skin lesions. Malassezia pachydermatis was the species most commonly cultured from the skin and external ear canal of healthy and diseased dogs; isolation of lipid-dependent yeasts from healthy dogs was less frequent. Using fungal culture as the gold standard, cytological examination showed good relative specificity (95%) but very low relative sensitivity (30%).

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Frequency, Body Distribution, and Population Size of Malassezia Species in Healthy Dogs and in Dogs with Localized Cutaneous Lesions

et al. 2005

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“…Lesions affecting the front feet, ear pinnae, ear pinna margins and dorsolumbar area were noted. After physical examination, the skin was sampled for the presence of Malassezia, by the acetate tape method applied to both axillae, both inguinal areas, and a skin lesion, if present (Bensignor et al, 2002;Machado et al, 2011).…”

Section: Methodsmentioning

confidence: 99%

Association between passive smoking and atopic dermatitis in dogs

Marignac

Desquilbet

et al. 2014

Food and Chemical Toxicology

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“…Sample collection and processing-For each dog, clear cellophane adhesive tape was used as described 6,7 to obtain a sample from the skin surface of the upper and lower eyelids of each eye for cytologic evaluation. Briefly, the upper and lower eyelids were held closed and a strip of adhesive tape was placed against the periocular skin of both eyelids simultaneously; gentle pressure was applied to the tape to ensure contact with the skin.…”

Section: Methodsmentioning

confidence: 99%

Malassezia spp on the periocular skin of dogs and their association with blepharitis, ocular discharge, and the application of ophthalmic medications

Newbold

Outerbridge²,

Kass

et al. 2014

javma

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Malassezia spp are lipophilic and often lipid-dependent yeasts that are common commensal organisms on the skin and ears of dogs but can also cause dermatologic and aural disease. 1 In veterinary medicine, one of the most clinically important species is Malassezia pachydermatis, which is an opportunistic pathogen commonly associated with canine dermatitis and otitis externa secondary to other diseases that result in abnormal sebaceous or ceruminous secretions. In dogs, the distribution of Malassezia spp is dependent on the location of skin sampled, disease status of the animal, and the method used for detection. Malassezia spp have been detected by cytologic evaluation in 40 of 99 (40%) healthy ears and 83 of 101 (82%) diseased ears, 2 and by culture of the organism from samples collected by the use of sterile cotton swabs moistened with sterile saline (0.9% NaCl)Malassezia spp on the periocular skin of dogs and their association with blepharitis, ocular discharge, and the application of ophthalmic medications Objective-To determine how frequently Malassezia spp were identified on the periocular skin of dogs and assess the respective associations between the presence of Malassezia spp on the periocular skin and blepharitis, ocular discharge, and the application of ophthalmic medications. Design-Prospective clinical study. Animals-167 eyelids of 84 dogs.Procedures-Samples obtained from the surface of the eyelid skin by use of adhesive tape were evaluated cytologically for the presence of Malassezia spp. Dogs were grouped on the basis of the presence of blepharitis, nature of ocular discharge, and whether ophthalmic medications were applied, and the proportion of samples with Malassezia spp was compared among the groups.Results-Malassezia spp were detected in 19 samples, of which 15 were obtained from eyes without blepharitis and 14 were obtained from eyes treated with topical ophthalmic medications. The proportion of samples with Malassezia spp was significantly higher for eyes with ocular discharge than for eyes without ocular discharge, especially if that discharge was mucoid or mucopurulent, and for eyes that were treated with aqueous-based medications only or a combination of oil-and aqueous-based medications than for eyes that were not treated. Conclusions and ClinicalRelevance-Malassezia organisms were detected on the periocular skin of 3 of 56 (5%) clinically normal dogs. Malassezia organisms were also frequently found on the periocular skin of dogs that had mucoid or mucopurulent ocular discharge or that were administered topical aqueous-based ophthalmic medications, and the periocular skin of these dogs should be cytologically evaluated for Malassezia organisms. (J Am Vet

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Comparison of two sampling techniques to assess quantity and distribution ofMalasseziayeasts on the skin of Basset Hounds

Cited by 35 publications

References 13 publications

Frequency, Body Distribution, and Population Size of Malassezia Species in Healthy Dogs and in Dogs with Localized Cutaneous Lesions

Frequency, Body Distribution, and Population Size of Malassezia Species in Healthy Dogs and in Dogs with Localized Cutaneous Lesions

Association between passive smoking and atopic dermatitis in dogs

Malassezia spp on the periocular skin of dogs and their association with blepharitis, ocular discharge, and the application of ophthalmic medications

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