Comparison of two simple microaerobic atmospheres for cultivation of thermophilic campylobacters.

Abstract

A comparison of a modification of the spirit burn method and one based on the Fortner principle was carried out on 3474 faecal samples from patients with diarrhoea. Of the 96 isolates of Campylobacter, 47 showed equal abundance of growth, regardless of the method used. By using the spirit burn method, however, growth of 33 isolates was significantly increased, and 18 out of those 33 isolates grew only in the spirit burn method atmosphere. The spirit burn method is more effective than the one based on the Fortner principle, it is easy to perform, quick, and cheap.

Changes in Campylobacter jejuni and Campylobacter coli Carriage Rates in the Zenica Canton of Bosnia and Herzegovina in the Pre- and Postwar Periods

“…The samples were collected from 340 children attending day care centers, 3,104 elementary school students, 221 high school students, and 1,540 adults. All samples were cultured on modified Preston medium (OXOID, Basingstoke, United Kingdom) and incubated in a microaerophilic atmosphere at 42°C for 48 h (3,5). C. jejuni and C. coli were identified using standard microbiological methods (6).…”

Some epidemiologic features of Campylobacter jejuni/coli infections in Bosnia and Herzegovina after the war

“…The number of patients examined in the laboratory was compared to the number of patients reported to the Cantonal Center for Public Health in Zenica as having diarrhea during January to December 1998 (Table 1). All stool samples were cultured on modified Preston medium (Oxoid, Basingstoke, UK), and incubated in a micro‐aerobic atmosphere at 42 °C for 48 h [4,5]. C. coli and C. jejuni were identified using standard microbiological methods [6].…”

Two Alternative Methods for Anaerobic Cultivation of Bacteroides Fragilis Atcc 25285 Compared to the Efficiency of Cultivation in a Gas Pack System

“…Some older methods of so-called physical and biological anaerobic cultivation are described in the literature. The biological method, which is called the Fortner principle, utilized the ability of rapidly growing facultative anaerobes to reduce the oxygen tension in a closed system, thus making possible the growth of oxygen-sensitive organisms [7,8] such as Bacteroides. The principle includes using an aerobic organism cultivated together with testing anaerobe into one nutrient medium.…”