Comparison of Two Swine <i>Mycoplasma Hyopneumoniae</i> Enzyme-Linked Immunosorbent Assays for Detection of Antibodies from Vaccinated Pigs and Field Serum Samples

Ameri-Mahabadi, Mehrdad; Zhou, En‐Min; Hsu, Walter H.

doi:10.1177/104063870501700112

Cited by 13 publications

(10 citation statements)

References 9 publications

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“…All blood by sampling day were centrifuged at 600 g for 15 min at 4 °C and the serum was analyzed for MHYO and PCV2 antibodies using respectively a monoclonal blocking ELISA Oxoid™ Mycoplasma hyopneumoniae (Oxoid LTD, UK) and an indirect ELISA Ingezim Circo IgG 1.1 PCV.K1 (Ingenasa, Spain), following manufacturers’ instructions. The Oxoid™ ELISA detects antibodies against the 74kD outer external protein of MHYO and is considered more sensitive than and at least as specific as other tests [35]. The Oxoid™ ELISA is less useful to measure antibody levels (it measures percentage of inhibition), but in our study working with conventional pigs that may have unspecific serum reactions for other Mycoplasma species [36], the Oxoid™ ELISA kit was selected.…”

Section: Methodsmentioning

confidence: 99%

Influence of spray dried porcine plasma in starter diets associated with a conventional vaccination program on wean to finish performance

et al. 2016

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BackgroundConventional vaccination programs using a single injection of a combined vaccine against porcine circovirus type 2 (PCV2) and Mycoplasma hyopneumoniae (MHYO) can promote a strong immune response that reduces feed intake for 24 to 48 h post injection. Often such vaccines are given around the time of weaning during a critical stress period in which feed intake is already compromised. Spray dried porcine plasma (SDPP) is a protein source used in starter diets that increases post-weaning feed intake of pigs. The objectives of this study were to determine the effects of a conventional vaccination program along with feeding SDPP in a starter diet on antibody development and wean to finish performance of pigs.ResultsPigs fed the starter diet with SDPP had improved body weight, average daily weight gain and average daily feed intake during the initial 14 d after weaning along with improved feed efficiency during the initial 7 d after weaning and these responses were independent of vaccination. Vaccination at 3 d after weaning had no significant effect on performance during the initial 14 d after weaning. Cumulative mortality was reduced for pigs fed the starter diet with SDPP, while vaccinated pigs had reduced mortality from d 48 to 145. Both vaccinated pigs and those fed the starter diet with SDPP had heavier carcass weight. One pig per pen was challenged with PCV2 at d 63. A higher percentage of vaccinated pigs were sero-positive for antibodies against PCV2 and MHYO at d 35, 63 and 78. Antibody values against PCV2 were higher for vaccinated pigs at d 35 and 63, but lower at d 146. Percentage of positive samples for PCV2 genome in serum was reduced for vaccinated pigs at d 117 and 146. Antibody values against MHYO were increased for vaccinated pigs at d 35, 63 and 78.ConclusionsVaccination supported a long term antibody response against PCV2 and a moderate but weaker antibody response against MHYO for early finishing pigs challenged with PCV2. Using SDPP in the starter diet along with vaccination supported the best long-term beneficial effects on survival to market and carcass weight.

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Section: Methodsmentioning

confidence: 99%

Influence of spray dried porcine plasma in starter diets associated with a conventional vaccination program on wean to finish performance

et al. 2016

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“…A blocking ELISA (IDEI, Mycoplasma hyopneumoniae EIA kit, Oxoid) and two indirect ELISA tests (HerdCheck, IDEXX and Tween 20-ELISA) are the most frequently used serological tests to detect antibodies to M. hyopneumoniae. Comparative studies have reported differing specificities and sensitivities between these kits (Pijoan, 1994;Strait et al, 2004;Ameri-Mahabadi et al, 2005;Erlandson, 2005). When discrepancies in serological test results are identified, a Western blot immunoassay (WBI) targeting different M. hyopneumoniae antigens can be used as a confirmatory test (Ameri et al, 2006).…”

Section: Serological Detection Of M Hyopneumoniae Infectionmentioning

confidence: 99%

Current perspectives on the diagnosis and epidemiology of Mycoplasma hyopneumoniae infection

Sibila

Pieters

Molitor

et al. 2009

The Veterinary Journal

157

127

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Mycoplasma hyopneumoniae is the principal aetiological agent of enzootic pneumonia (EP), a chronic respiratory disease that affects mainly finishing pigs. Although major efforts to control M. hyopneumoniae infection and its detrimental effects have been made, significant economic losses in pig production worldwide due to EP continue. M. hyopneumoniae is typically introduced into pig herds by the purchase of subclinically infected animals or, less frequently, through airborne transmission over short distances. Once in the herd, M. hyopneumoniae may be transmitted by direct contact from infected sows to their offspring or between pen mates. The 'gold standard' technique used to diagnose M. hyopneumoniae infection, bacteriological culture, is laborious and is seldom used routinely. Enzyme-linked immunosorbent assay and polymerase chain reaction detection methods, in addition to post-mortem inspection in the form of abattoir surveillance or field necropsy, are the techniques most frequently used to investigate the potential involvement of M. hyopneumoniae in porcine respiratory disease. Such techniques have been used to monitor the incidence of M. hyopneumoniae infection in herds both clinically and subclinically affected by EP, in vaccinated and non-vaccinated herds and under different production and management conditions. Differences in the clinical course of EP at farm level and in the efficacy of M. hyopneumoniae vaccination suggest that the transmission and virulence characteristics of different field isolates of M. hyopneumoniae may vary. This paper reviews the current state of knowledge of the epidemiology of M. hyopneumoniae infection including its transmission, infection and seroconversion dynamics and also compares the various epidemiological tools used to monitor EP.

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“…Kappa coefficients for agreement obtained in the current study were higher than those described in previous studies 5 ; however, the kappa coefficients were similar to those described in previous studies comparing the blocking ELISA with another indirect ELISA. 1 This information is important with regards to interpretation of results in field cases. Knowledge of the optimal time for testing, according to the test being used, helps the practitioner to determine the best test according to the stage of infection.…”

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confidence: 99%

“…5 In another independent study, the Dako ELISA assay was also compared to another M. hyopneumoniae indirect ELISA b showing that the Dako ELISA was more successful in identifying positive samples. 1 It has been shown that sensitivity of these serological tests is influenced by the infection stage of the host, suggesting that these assays are less sensitive early in infection. 5 However, information on the performance of these serological tests in repeatedly measured challenged pigs has not been extensively reported in the literature.…”

mentioning

confidence: 99%

Longitudinal assessment of two Mycoplasma hyopneumoniae enzyme-linked immunosorbent assays in challenged and contact-exposed pigs

et al. 2012

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Abstract. The objective of the current study was to compare the sensitivity of 2 Mycoplasma hyopneumoniae enzymelinked immunosorbent assays (ELISAs) in experimentally challenged and contact-exposed pigs in a long-term longitudinal assessment. On day 0 of the assessment, twelve 2-month-old M. hyopneumoniae-negative pigs were inoculated with M. hyopneumoniae strain 232 (group A). Twelve negative pigs were placed alongside the inoculated pigs, allowing direct contact exposure (group B). A third group of 12 pigs was allocated into 2 independent pens; no direct contact was allowed (group C). A longitudinal serologic profile was performed; samples were collected on days 0, 28, 35, 42, 49, 63, 91, 119, 154, and 170 of the study. Serum samples were tested using a blocking ELISA and an indirect ELISA. Results of the study demonstrated higher sensitivity of the blocking ELISA during early infection (clinical signs). Both ELISAs were 100% sensitive in challenged and naturally infected groups at several testing points during late infection (63, 91, 119, 154, and 170 days of the study) and showed a long antibody detection period. Both tests worked equally well during the chronic phase of infection but the blocking ELISA was more sensitive during acute stages of infection.

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Comparison of Two Swine Mycoplasma Hyopneumoniae Enzyme-Linked Immunosorbent Assays for Detection of Antibodies from Vaccinated Pigs and Field Serum Samples

Cited by 13 publications

References 9 publications

Influence of spray dried porcine plasma in starter diets associated with a conventional vaccination program on wean to finish performance

Influence of spray dried porcine plasma in starter diets associated with a conventional vaccination program on wean to finish performance

Current perspectives on the diagnosis and epidemiology of Mycoplasma hyopneumoniae infection

Longitudinal assessment of two Mycoplasma hyopneumoniae enzyme-linked immunosorbent assays in challenged and contact-exposed pigs

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